Diversity of gammadelta T cells in patients with Behcet's disease is indicative of polyclonal activation

2.50
Hdl Handle:
http://hdl.handle.net/2336/10607
Title:
Diversity of gammadelta T cells in patients with Behcet's disease is indicative of polyclonal activation
Authors:
Freysdottir, J; Hussain, L; Farmer, I; Lau, S-H; Fortune, F
Citation:
Oral Dis 2006, 12(3):271-7
Issue Date:
1-May-2006
Abstract:
OBJECTIVE: Behcet's disease (BD) is a multisystemic disease, with vasculitic lesions in the oral and genital mucosa, eyes, joints, skin and brain. We have previously found that gammadelta T cells are increased in peripheral blood of BD patients. The aim of this study was to investigate the extent of gammadelta T cells in oral biopsies from BD patients with special emphasis on the restriction of Vgamma and Vdelta usage. PATIENTS AND METHODS: Expression of Vgamma and Vdelta chains on peripheral blood gammadelta T cells from 31 BD patients and 19 healthy controls was analysed by flow cytometry and the expression of Vgamma and Vdelta chains in nine ulcerated and eight non-ulcerated oral mucosa from BD patients and non-ulcerated oral mucosa from three healthy controls was analysed by immunohistochemistry. RESULTS: Vgamma9 and Vdelta2 were the predominant chains expressed in peripheral blood of BD patients, although other Vgamma and Vdelta chains were also expressed. The presence of gammadelta T cells was only observed in the ulcerated oral mucosa but not in the non-ulcerated mucosa from the BD patients, and not in the non-ulcerated mucosa from the healthy controls. These gammadelta T cells showed no preferential expression of any of the Vgamma or Vdelta chains. CONCLUSION: These data suggest a polyclonal rather than oligoclonal activation of the gammadelta T cells. This may indicate that during repeated inflammation of the oral mucosa, the gammadelta T cells are responding to a wide variety of antigenic stimuli with consequent expansion of gammadelta T cells expressing various Vgamma and Vdelta chains and that different antigenic stimuli or responses may be responsible for the clinical heterogeneity of the disease.
Description:
To access publisher full text version of this article. Please click on the hyperlink in Additional Links field
Additional Links:
http://www.blackwell-synergy.com/doi/abs/10.1111/j.1601-0825.2005.01185.x

Full metadata record

DC FieldValue Language
dc.contributor.authorFreysdottir, J-
dc.contributor.authorHussain, L-
dc.contributor.authorFarmer, I-
dc.contributor.authorLau, S-H-
dc.contributor.authorFortune, F-
dc.date.accessioned2007-03-13T08:41:45Z-
dc.date.available2007-03-13T08:41:45Z-
dc.date.issued2006-05-01-
dc.date.submitted2007-03-13-
dc.identifier.citationOral Dis 2006, 12(3):271-7en
dc.identifier.issn1354-523X-
dc.identifier.pmid16700735-
dc.identifier.doi10.1111/j.1601-0825.2005.01185.x-
dc.identifier.urihttp://hdl.handle.net/2336/10607-
dc.descriptionTo access publisher full text version of this article. Please click on the hyperlink in Additional Links fielden
dc.description.abstractOBJECTIVE: Behcet's disease (BD) is a multisystemic disease, with vasculitic lesions in the oral and genital mucosa, eyes, joints, skin and brain. We have previously found that gammadelta T cells are increased in peripheral blood of BD patients. The aim of this study was to investigate the extent of gammadelta T cells in oral biopsies from BD patients with special emphasis on the restriction of Vgamma and Vdelta usage. PATIENTS AND METHODS: Expression of Vgamma and Vdelta chains on peripheral blood gammadelta T cells from 31 BD patients and 19 healthy controls was analysed by flow cytometry and the expression of Vgamma and Vdelta chains in nine ulcerated and eight non-ulcerated oral mucosa from BD patients and non-ulcerated oral mucosa from three healthy controls was analysed by immunohistochemistry. RESULTS: Vgamma9 and Vdelta2 were the predominant chains expressed in peripheral blood of BD patients, although other Vgamma and Vdelta chains were also expressed. The presence of gammadelta T cells was only observed in the ulcerated oral mucosa but not in the non-ulcerated mucosa from the BD patients, and not in the non-ulcerated mucosa from the healthy controls. These gammadelta T cells showed no preferential expression of any of the Vgamma or Vdelta chains. CONCLUSION: These data suggest a polyclonal rather than oligoclonal activation of the gammadelta T cells. This may indicate that during repeated inflammation of the oral mucosa, the gammadelta T cells are responding to a wide variety of antigenic stimuli with consequent expansion of gammadelta T cells expressing various Vgamma and Vdelta chains and that different antigenic stimuli or responses may be responsible for the clinical heterogeneity of the disease.en
dc.language.isoenen
dc.relation.urlhttp://www.blackwell-synergy.com/doi/abs/10.1111/j.1601-0825.2005.01185.xen
dc.subject.meshAntigenic Variationen
dc.subject.meshAntigens, CD3en
dc.subject.meshBehcet Syndromeen
dc.subject.meshCase-Control Studiesen
dc.subject.meshHumansen
dc.subject.meshImmunohistochemistryen
dc.subject.meshLymphocyte Activationen
dc.subject.meshMouth Mucosaen
dc.subject.meshOral Ulceren
dc.subject.meshReceptors, Antigen, T-Cell, gamma-deltaen
dc.subject.meshT-Lymphocytesen
dc.titleDiversity of gammadelta T cells in patients with Behcet's disease is indicative of polyclonal activationen
dc.typeArticleen
dc.identifier.journalOral diseasesen
dc.identifier.journalOral diseasesen
dc.format.digYES-

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