Effect of hypoxia and TP53 mutation status and cytogenetics of normal and malignant mammary epithelium

2.50
Hdl Handle:
http://hdl.handle.net/2336/10646
Title:
Effect of hypoxia and TP53 mutation status and cytogenetics of normal and malignant mammary epithelium
Authors:
Vidarsson, Hilmar; Steinarsdóttir, Margrét; Jónasson, Jón Gunnlaugur; Júlíusdóttir, Hildur; Hauksdóttir, Halla; Hilmarsdóttir, Hólmfrídur; Halldórsdóttir, Kristín; Ogmundsdóttir, Helga M
Citation:
Cancer Genet. Cytogenet. 2006, 165(2):144-50
Issue Date:
1-Mar-2006
Abstract:
It has been proposed that hypoxia favors the growth of tumor cells over normal cells, particularly tumor cells carrying TP53 mutations. Cytogenetic studies of breast cancer have shown that highly complex karyotypes seen in direct harvest preparations are rarely detected after short-term culture. In this study, 34 paired samples of breast carcinomas and grossly nontumorous tissue from the same breast were cultured at 20 and 5% (12 samples) or 20 and 0% oxygen (22 samples). Both carcinoma samples and nontumorous tissue survived at 0% oxygen. Recovery for 24 hours at 20% produced good yields for cytogenetic analysis. Lower oxygen levels did not specifically stimulate growth of tumor cells. Samples with TP53 mutations showed a consistently increased growth under anaerobic hypoxic conditions. Culture at 5% oxygen did not generally reveal more karyotypic abnormalities than found at 20%. In the samples cultured at 0 and 20%, karyotypic abnormalities were detected only in anaerobic hypoxic culture in two cases. Of the only four samples where more complex karyotypes were detected in the low-oxygen culture, two were TP53 mutated. Hypoxic treatment followed by recovery at 20% oxygen may thus increase the yield of complex karyotypes from a subset of breast carcinomas, particularly those with mutated TP53.
Description:
To access publisher full text version of this article. Please click on the hyperlink in Additional Links field
Additional Links:
http://www.sciencedirect.com/science/article/B6T53-4JF38TD-9/2/a2071ed7a1703613a837f50fa32d6794

Full metadata record

DC FieldValue Language
dc.contributor.authorVidarsson, Hilmar-
dc.contributor.authorSteinarsdóttir, Margrét-
dc.contributor.authorJónasson, Jón Gunnlaugur-
dc.contributor.authorJúlíusdóttir, Hildur-
dc.contributor.authorHauksdóttir, Halla-
dc.contributor.authorHilmarsdóttir, Hólmfrídur-
dc.contributor.authorHalldórsdóttir, Kristín-
dc.contributor.authorOgmundsdóttir, Helga M-
dc.date.accessioned2007-03-14T09:38:56Z-
dc.date.available2007-03-14T09:38:56Z-
dc.date.issued2006-03-01-
dc.date.submitted2007-03-14-
dc.identifier.citationCancer Genet. Cytogenet. 2006, 165(2):144-50en
dc.identifier.issn0165-4608-
dc.identifier.pmid16527608-
dc.identifier.doi10.1016/j.cancergencyto.2005.07.024-
dc.identifier.otherPTT12-
dc.identifier.urihttp://hdl.handle.net/2336/10646-
dc.descriptionTo access publisher full text version of this article. Please click on the hyperlink in Additional Links fielden
dc.description.abstractIt has been proposed that hypoxia favors the growth of tumor cells over normal cells, particularly tumor cells carrying TP53 mutations. Cytogenetic studies of breast cancer have shown that highly complex karyotypes seen in direct harvest preparations are rarely detected after short-term culture. In this study, 34 paired samples of breast carcinomas and grossly nontumorous tissue from the same breast were cultured at 20 and 5% (12 samples) or 20 and 0% oxygen (22 samples). Both carcinoma samples and nontumorous tissue survived at 0% oxygen. Recovery for 24 hours at 20% produced good yields for cytogenetic analysis. Lower oxygen levels did not specifically stimulate growth of tumor cells. Samples with TP53 mutations showed a consistently increased growth under anaerobic hypoxic conditions. Culture at 5% oxygen did not generally reveal more karyotypic abnormalities than found at 20%. In the samples cultured at 0 and 20%, karyotypic abnormalities were detected only in anaerobic hypoxic culture in two cases. Of the only four samples where more complex karyotypes were detected in the low-oxygen culture, two were TP53 mutated. Hypoxic treatment followed by recovery at 20% oxygen may thus increase the yield of complex karyotypes from a subset of breast carcinomas, particularly those with mutated TP53.en
dc.language.isoenen
dc.publisherElsevier Scienceen
dc.relation.urlhttp://www.sciencedirect.com/science/article/B6T53-4JF38TD-9/2/a2071ed7a1703613a837f50fa32d6794en
dc.subject.meshBreasten
dc.subject.meshBreast Neoplasmsen
dc.subject.meshCell Divisionen
dc.subject.meshCell Hypoxiaen
dc.subject.meshImmunohistochemistryen
dc.subject.meshKaryotypingen
dc.subject.meshMutationen
dc.subject.meshTumor Suppressor Protein p53en
dc.titleEffect of hypoxia and TP53 mutation status and cytogenetics of normal and malignant mammary epitheliumen
dc.typeArticleen
dc.identifier.journalCancer genetics and cytogeneticsen
dc.format.digYES-

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