Interstitial deletions including chromosome 3 common eliminated region 1 (C3CER1) prevail in human solid tumors from 10 different tissues

2.50
Hdl Handle:
http://hdl.handle.net/2336/13592
Title:
Interstitial deletions including chromosome 3 common eliminated region 1 (C3CER1) prevail in human solid tumors from 10 different tissues
Authors:
Petursdottir, Thorgunnur Eyfjord; Thorsteinsdottir, Unnur; Jonasson, Jon Gunnlaugur; Moller, Pall Helgi; Huiping, Chen; Bjornsson, Johannes; Egilsson, Valgardur; Imreh, Stefan; Ingvarsson, Sigurdur
Citation:
Genes Chromosomes Cancer 2004, 41(3):232-42
Issue Date:
1-Nov-2004
Abstract:
A human chromosomal segment regularly lost during tumor formation of microcell hybrids in SCID mice has been mapped to 3p21.3. This segment, called chromosome 3 common eliminated region 1 (C3CER1, also referred to as CER1), may harbor multiple tumor-suppressor genes. Because it was found that similar regions were eliminated in an inter- and intraspecies system and in two tumor types (mouse fibrosarcoma and human renal cell carcinoma), we hypothesized that the importance of C3CER1 would transgress tissue specificity, that is, it could occur in tumors derived from multiple tissues. To evaluate the loss of C3CER1 in various human tumor types, we conducted loss of heterozygosity (LOH) analysis of 576 human solid tumors from 10 different tissues and compared the frequency of deletion in the C3CER1 area to that in two other regions on 3p: the FHIT/FRA3B region, at 3p14.2, and the VHL region, at 3p25.3. Deletions were detected in the C3CER1 region in 83% of informative tumors. Half (47%) the LOH-positive tumors showed LOH at all informative markers, indicating a large deletion. The other half (53%) had a discontinuous LOH pattern, suggesting interstitial deletions or breakpoints. The proportion of tumors with C3CER1 deletions was high in all tumor types investigated, ranging from 70% to 94%, except for the soft-tissue sarcomas (40%). In the VHL and FHIT regions, deletions were observed in 73% and 43%, respectively, of the tumors. Of the three 3p regions analyzed, the highest deletion frequency was observed in the C3CER1 region. Furthermore, we demonstrated that the interstitial deletions including C3CER1 prevail over 3p14.2-pter losses in solid tumors.
Description:
To access publisher full text version of this article. Please click on the hyperlink in Additional Links field
Additional Links:
http://dx.doi.org/10.1002/gcc.20072

Full metadata record

DC FieldValue Language
dc.contributor.authorPetursdottir, Thorgunnur Eyfjord-
dc.contributor.authorThorsteinsdottir, Unnur-
dc.contributor.authorJonasson, Jon Gunnlaugur-
dc.contributor.authorMoller, Pall Helgi-
dc.contributor.authorHuiping, Chen-
dc.contributor.authorBjornsson, Johannes-
dc.contributor.authorEgilsson, Valgardur-
dc.contributor.authorImreh, Stefan-
dc.contributor.authorIngvarsson, Sigurdur-
dc.date.accessioned2007-09-11T14:35:58Z-
dc.date.available2007-09-11T14:35:58Z-
dc.date.issued2004-11-01-
dc.date.submitted2007-09-11-
dc.identifier.citationGenes Chromosomes Cancer 2004, 41(3):232-42en
dc.identifier.issn1045-2257-
dc.identifier.pmid15334546-
dc.identifier.doi10.1002/gcc.20072-
dc.identifier.otherPTT12-
dc.identifier.urihttp://hdl.handle.net/2336/13592-
dc.descriptionTo access publisher full text version of this article. Please click on the hyperlink in Additional Links fielden
dc.description.abstractA human chromosomal segment regularly lost during tumor formation of microcell hybrids in SCID mice has been mapped to 3p21.3. This segment, called chromosome 3 common eliminated region 1 (C3CER1, also referred to as CER1), may harbor multiple tumor-suppressor genes. Because it was found that similar regions were eliminated in an inter- and intraspecies system and in two tumor types (mouse fibrosarcoma and human renal cell carcinoma), we hypothesized that the importance of C3CER1 would transgress tissue specificity, that is, it could occur in tumors derived from multiple tissues. To evaluate the loss of C3CER1 in various human tumor types, we conducted loss of heterozygosity (LOH) analysis of 576 human solid tumors from 10 different tissues and compared the frequency of deletion in the C3CER1 area to that in two other regions on 3p: the FHIT/FRA3B region, at 3p14.2, and the VHL region, at 3p25.3. Deletions were detected in the C3CER1 region in 83% of informative tumors. Half (47%) the LOH-positive tumors showed LOH at all informative markers, indicating a large deletion. The other half (53%) had a discontinuous LOH pattern, suggesting interstitial deletions or breakpoints. The proportion of tumors with C3CER1 deletions was high in all tumor types investigated, ranging from 70% to 94%, except for the soft-tissue sarcomas (40%). In the VHL and FHIT regions, deletions were observed in 73% and 43%, respectively, of the tumors. Of the three 3p regions analyzed, the highest deletion frequency was observed in the C3CER1 region. Furthermore, we demonstrated that the interstitial deletions including C3CER1 prevail over 3p14.2-pter losses in solid tumors.en
dc.language.isoenen
dc.publisherWiley-Lissen
dc.relation.urlhttp://dx.doi.org/10.1002/gcc.20072en
dc.subject.meshAcid Anhydride Hydrolasesen
dc.subject.meshCell Line, Tumoren
dc.subject.meshChromosomes, Human, Pair 3en
dc.subject.meshGene Deletionen
dc.subject.meshLoss of Heterozygosityen
dc.subject.meshModels, Geneticen
dc.subject.meshNeoplasm Proteinsen
dc.subject.meshNeoplasmsen
dc.subject.meshTumor Markers, Biologicalen
dc.subject.meshTumor Suppressor Proteinsen
dc.subject.meshUbiquitin-Protein Ligasesen
dc.subject.meshVon Hippel-Lindau Tumor Suppressor Proteinen
dc.titleInterstitial deletions including chromosome 3 common eliminated region 1 (C3CER1) prevail in human solid tumors from 10 different tissuesen
dc.typeArticleen
dc.format.digYES-
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