Peripheral blood T cell responses to keratin peptides that share sequences with streptococcal M proteins are largely restricted to skin-homing CD8(+) T cells.

2.50
Hdl Handle:
http://hdl.handle.net/2336/13593
Title:
Peripheral blood T cell responses to keratin peptides that share sequences with streptococcal M proteins are largely restricted to skin-homing CD8(+) T cells.
Authors:
Johnston, A; Gudjonsson, J E; Sigmundsdottir, H; Love, T J; Valdimarsson, H
Citation:
Clin. Exp. Immunol. 2004, 138(1):83-93
Issue Date:
1-Oct-2004
Abstract:
The association of psoriasis with Streptococcus pyogenes throat infections suggests a potential antigenic target for the T cells that are known to infiltrate psoriatic skin. Streptococcal M protein share an extensive sequence homology with the human epidermal keratins. Keratin 17 (K17), while being mostly absent from uninvolved skin, is up-regulated in psoriatic lesions. Consequentially, M-protein-primed T cells may recognize up-regulated keratin epitopes via molecular mimicry. Using in vitro lymphocyte culture and cytokine flow cytometry we demonstrate that HLA-Cw*0602(+) psoriasis patients had significant CD8(+) T cell interferon (IFN)-gamma responses to peptides from the K17 and M6 protein selected on the basis of sequence homology and predicted HLA-Cw*0602 binding. These responses were about 10 times more frequent in the skin-homing cutaneous lymphocyte-associated antigen-expressing (CLA(+)) subset of CD8(+) T cells. CD4(+) T cells showed only borderline responses. CLA(+) CD8(+) T cells from Cw6(+) non-psoriatic individuals responded to some M6 peptides but rarely to K17 peptides. Cw6(-) psoriasis patients showed a response that was intermediate between Cw6(+) patients and controls. These findings indicate that psoriatic individuals have CD8(+) T cells that recognize keratin self-antigens and that epitopes shared by streptococcal M proteins and human keratins may be targets for the CD8(+) T cells that infiltrate psoriatic skin lesions.
Description:
To access Publisher full text version of this article. Please click on the hyperlink in Additional Links field
Additional Links:
http://www.blackwell-synergy.com/doi/abs/10.1111/j.1365-2249.2004.00600.x

Full metadata record

DC FieldValue Language
dc.contributor.authorJohnston, A-
dc.contributor.authorGudjonsson, J E-
dc.contributor.authorSigmundsdottir, H-
dc.contributor.authorLove, T J-
dc.contributor.authorValdimarsson, H-
dc.date.accessioned2007-09-11T14:55:15Z-
dc.date.available2007-09-11T14:55:15Z-
dc.date.issued2004-10-01-
dc.identifier.citationClin. Exp. Immunol. 2004, 138(1):83-93en
dc.identifier.issn0009-9104-
dc.identifier.pmid15373909-
dc.identifier.doi10.1111/j.1365-2249.2004.00600.x-
dc.identifier.otherAAI12-
dc.identifier.urihttp://hdl.handle.net/2336/13593-
dc.descriptionTo access Publisher full text version of this article. Please click on the hyperlink in Additional Links fielden
dc.description.abstractThe association of psoriasis with Streptococcus pyogenes throat infections suggests a potential antigenic target for the T cells that are known to infiltrate psoriatic skin. Streptococcal M protein share an extensive sequence homology with the human epidermal keratins. Keratin 17 (K17), while being mostly absent from uninvolved skin, is up-regulated in psoriatic lesions. Consequentially, M-protein-primed T cells may recognize up-regulated keratin epitopes via molecular mimicry. Using in vitro lymphocyte culture and cytokine flow cytometry we demonstrate that HLA-Cw*0602(+) psoriasis patients had significant CD8(+) T cell interferon (IFN)-gamma responses to peptides from the K17 and M6 protein selected on the basis of sequence homology and predicted HLA-Cw*0602 binding. These responses were about 10 times more frequent in the skin-homing cutaneous lymphocyte-associated antigen-expressing (CLA(+)) subset of CD8(+) T cells. CD4(+) T cells showed only borderline responses. CLA(+) CD8(+) T cells from Cw6(+) non-psoriatic individuals responded to some M6 peptides but rarely to K17 peptides. Cw6(-) psoriasis patients showed a response that was intermediate between Cw6(+) patients and controls. These findings indicate that psoriatic individuals have CD8(+) T cells that recognize keratin self-antigens and that epitopes shared by streptococcal M proteins and human keratins may be targets for the CD8(+) T cells that infiltrate psoriatic skin lesions.en
dc.language.isoenen
dc.publisherBlackwell Scientific Publicationsen
dc.relation.urlhttp://www.blackwell-synergy.com/doi/abs/10.1111/j.1365-2249.2004.00600.xen
dc.subject.meshAntigens, Bacterialen
dc.subject.meshBacterial Outer Membrane Proteinsen
dc.subject.meshKeratinsen
dc.subject.meshStreptococcus pyogenesen
dc.subject.meshSequence Homology, Amino Aciden
dc.subject.meshPsoriasisen
dc.titlePeripheral blood T cell responses to keratin peptides that share sequences with streptococcal M proteins are largely restricted to skin-homing CD8(+) T cells.en
dc.typeArticleen
dc.identifier.journalClinical and experimental immunologyen
dc.format.digYES-

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