Flt3/Flk-2 ligand in combination with thrombopoietin decreases apoptosis in megakaryocyte development

2.50
Hdl Handle:
http://hdl.handle.net/2336/15736
Title:
Flt3/Flk-2 ligand in combination with thrombopoietin decreases apoptosis in megakaryocyte development
Authors:
Sigurjonsson, Olafur Eysteinn; Gudmundsson, Kristbjorn Orri; Haraldsdottir, Vilhelmina; Rafnar, Thorunn; Agnarsson, Bjarni A; Gudmundsson, Sveinn
Citation:
Stem Cells Dev. 2004, 13(2):183-91
Issue Date:
1-Apr-2004
Abstract:
The growth factors thrombopoietin (TPO) and Flt3/Flk-2-ligand (FL), either independently or in combination, modulate megakaryocyte development. Our results show that bone marrow CD34+ cells cultured with TPO and FL differentiate at a slower rate into CD41+ cells and are delayed in apoptosis at the later stages of the cultures compared to cells cultured with TPO alone. Our data also show that FL in synergy with TPO may inhibit apoptosis in megakaryocyte development by up-regulating bcl-2 and inducing conformational changes of p53, in MK progenitors. FL in combination with TPO slows down maturation and consequently delays apoptosis of MK progenitor cells.
Description:
To access publisher full text version of this article. Please click on the hyperlink in Additional Links field
Additional Links:
http://www.liebertonline.com/doi/abs/10.1089/154732804323046783

Full metadata record

DC FieldValue Language
dc.contributor.authorSigurjonsson, Olafur Eysteinn-
dc.contributor.authorGudmundsson, Kristbjorn Orri-
dc.contributor.authorHaraldsdottir, Vilhelmina-
dc.contributor.authorRafnar, Thorunn-
dc.contributor.authorAgnarsson, Bjarni A-
dc.contributor.authorGudmundsson, Sveinn-
dc.date.accessioned2008-01-07T11:25:51Z-
dc.date.available2008-01-07T11:25:51Z-
dc.date.issued2004-04-01-
dc.date.submitted2007-01-07-
dc.identifier.citationStem Cells Dev. 2004, 13(2):183-91en
dc.identifier.issn1547-3287-
dc.identifier.pmid15186734-
dc.identifier.doi10.1089/154732804323046783-
dc.identifier.urihttp://hdl.handle.net/2336/15736-
dc.descriptionTo access publisher full text version of this article. Please click on the hyperlink in Additional Links fielden
dc.description.abstractThe growth factors thrombopoietin (TPO) and Flt3/Flk-2-ligand (FL), either independently or in combination, modulate megakaryocyte development. Our results show that bone marrow CD34+ cells cultured with TPO and FL differentiate at a slower rate into CD41+ cells and are delayed in apoptosis at the later stages of the cultures compared to cells cultured with TPO alone. Our data also show that FL in synergy with TPO may inhibit apoptosis in megakaryocyte development by up-regulating bcl-2 and inducing conformational changes of p53, in MK progenitors. FL in combination with TPO slows down maturation and consequently delays apoptosis of MK progenitor cells.en
dc.language.isoenen
dc.publisherMary Ann Liebert, Inc.en
dc.relation.urlhttp://www.liebertonline.com/doi/abs/10.1089/154732804323046783en
dc.subject.meshAntigens, CD95en
dc.subject.meshApoptosisen
dc.subject.meshCell Differentiationen
dc.subject.meshDrug Synergismen
dc.subject.meshHumansen
dc.subject.meshMegakaryocytesen
dc.subject.meshMembrane Proteinsen
dc.subject.meshPlatelet Membrane Glycoprotein IIben
dc.subject.meshProto-Oncogene Proteins c-bcl-2en
dc.subject.meshThrombopoietinen
dc.subject.meshTumor Suppressor Protein p53en
dc.titleFlt3/Flk-2 ligand in combination with thrombopoietin decreases apoptosis in megakaryocyte developmenten
dc.typeArticleen
dc.identifier.journalStem cells and developmenten

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