A genome-wide study of allelic imbalance in human testicular germ cell tumors using microsatellite markers

2.50
Hdl Handle:
http://hdl.handle.net/2336/15853
Title:
A genome-wide study of allelic imbalance in human testicular germ cell tumors using microsatellite markers
Authors:
Bergthorsson, Jon Thor; Agnarsson, Bjarni Agnar; Gudbjartsson, Tomas; Magnusson, Kjartan; Thoroddsen, Asgeir; Palsson, Birgir; Bjornsson, Johannes; Stefansson, Kari; Gulcher, Jeffrey; Einarsson, Gudmundur Vikar; Amundadottir, Laufey Thora; Barkardottir, Rosa Bjork
Citation:
Cancer Genet. Cytogenet. 2006, 164(1):1-9
Issue Date:
1-Jan-2006
Abstract:
Testicular germ cell tumors (TGCT) arise by multistep carcinogenesis pathways involving selective losses and gains of chromosome material. To locate cancer genes underlying this selection, we performed a genome-wide study of allelic imbalance (AI) in 32 tumors, using 710 microsatellite markers. The highest prevalence of AI was found at 12p, in line with previous studies finding consistent gain of the region in TGCTs. High frequency of AI was also observed at chromosome arms 4p, 9q, 10p, 11q, 11p, 13q, 16q, 18p, and 22q. Within 39 candidate regions identified by mapping of smallest regions of overlap (SROs), the highest frequency of AI was at 12p11.21 approximately p11.22 (62%), 12p12.1 approximately p13.1 (53%), 12p13.1 approximately p13.2 (53%), 11q14.1 approximately q14.2 (53%), 11p13 approximately p14.3 (47%), 9q21.13 approximately q21.32 (47%), and 4p15.1 approximately p15.2 (44%). Two genes known to be involved in cancer reside in these regions, ETV6 at 12p13.2 (TEL oncogene) and WT1 at 11p13. We also found a significant association (P = 0.02) between AI at 10q21.1 approximately q22.2 and higher clinical stage. This study contributes to the ongoing search for genes involved in transformation of germ cells and provides a useful reference point to previous studies using cytogenetic techniques to map chromosome changes in TGCTs.
Description:
To access publisher full text version of this article. Please click on the hyperlink in Additional Links field
Additional Links:
http://www.sciencedirect.com/science/article/B6T53-4HTV1CX-3/2/0dbe007455072f6ffab29c974cb9e4e1

Full metadata record

DC FieldValue Language
dc.contributor.authorBergthorsson, Jon Thor-
dc.contributor.authorAgnarsson, Bjarni Agnar-
dc.contributor.authorGudbjartsson, Tomas-
dc.contributor.authorMagnusson, Kjartan-
dc.contributor.authorThoroddsen, Asgeir-
dc.contributor.authorPalsson, Birgir-
dc.contributor.authorBjornsson, Johannes-
dc.contributor.authorStefansson, Kari-
dc.contributor.authorGulcher, Jeffrey-
dc.contributor.authorEinarsson, Gudmundur Vikar-
dc.contributor.authorAmundadottir, Laufey Thora-
dc.contributor.authorBarkardottir, Rosa Bjork-
dc.date.accessioned2008-01-09T09:17:23Z-
dc.date.available2008-01-09T09:17:23Z-
dc.date.issued2006-01-01-
dc.date.submitted2007-01-09-
dc.identifier.citationCancer Genet. Cytogenet. 2006, 164(1):1-9en
dc.identifier.issn0165-4608-
dc.identifier.pmid16364756-
dc.identifier.doi10.1016/j.cancergencyto.2005.06.015-
dc.identifier.urihttp://hdl.handle.net/2336/15853-
dc.descriptionTo access publisher full text version of this article. Please click on the hyperlink in Additional Links fielden
dc.description.abstractTesticular germ cell tumors (TGCT) arise by multistep carcinogenesis pathways involving selective losses and gains of chromosome material. To locate cancer genes underlying this selection, we performed a genome-wide study of allelic imbalance (AI) in 32 tumors, using 710 microsatellite markers. The highest prevalence of AI was found at 12p, in line with previous studies finding consistent gain of the region in TGCTs. High frequency of AI was also observed at chromosome arms 4p, 9q, 10p, 11q, 11p, 13q, 16q, 18p, and 22q. Within 39 candidate regions identified by mapping of smallest regions of overlap (SROs), the highest frequency of AI was at 12p11.21 approximately p11.22 (62%), 12p12.1 approximately p13.1 (53%), 12p13.1 approximately p13.2 (53%), 11q14.1 approximately q14.2 (53%), 11p13 approximately p14.3 (47%), 9q21.13 approximately q21.32 (47%), and 4p15.1 approximately p15.2 (44%). Two genes known to be involved in cancer reside in these regions, ETV6 at 12p13.2 (TEL oncogene) and WT1 at 11p13. We also found a significant association (P = 0.02) between AI at 10q21.1 approximately q22.2 and higher clinical stage. This study contributes to the ongoing search for genes involved in transformation of germ cells and provides a useful reference point to previous studies using cytogenetic techniques to map chromosome changes in TGCTs.en
dc.language.isoenen
dc.publisherElsevier/North-Hollanden
dc.relation.urlhttp://www.sciencedirect.com/science/article/B6T53-4HTV1CX-3/2/0dbe007455072f6ffab29c974cb9e4e1en
dc.subject.meshAdolescenten
dc.subject.meshAdulten
dc.subject.meshAllelic Imbalanceen
dc.subject.meshChild, Preschoolen
dc.subject.meshGenes, Wilms Tumoren
dc.subject.meshGenomeen
dc.subject.meshHumansen
dc.subject.meshMaleen
dc.subject.meshMicrosatellite Repeatsen
dc.subject.meshNeoplasm Stagingen
dc.subject.meshNeoplasms, Germ Cell and Embryonalen
dc.subject.meshNucleic Acid Hybridizationen
dc.subject.meshProto-Oncogene Proteins c-etsen
dc.subject.meshRepressor Proteinsen
dc.subject.meshTesticular Neoplasmsen
dc.titleA genome-wide study of allelic imbalance in human testicular germ cell tumors using microsatellite markersen
dc.typeArticleen
dc.identifier.eissn1873-4456-
dc.identifier.journalCancer genetics and cytogeneticsen

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