Differentiation potential of a basal epithelial cell line established from human bronchial explant.

2.50
Hdl Handle:
http://hdl.handle.net/2336/18658
Title:
Differentiation potential of a basal epithelial cell line established from human bronchial explant.
Authors:
Halldorsson, Skarphedinn; Asgrimsson, Valthor; Axelsson, Ivar; Gudmundsson, Gudmundur Hrafn; Steinarsdottir, Margret; Baldursson, Olafur; Gudjonsson, Thorarinn
Citation:
In Vitro Cell. Dev. Biol. Anim. 2007, 43(8-9):283-9
Issue Date:
1-Oct-2007
Abstract:
Due to the cellular complexity of the airway epithelium, it is important to carefully define bronchial cell lines that capture the phenotypic traits of a particular cell type. We describe the characterization of a human bronchial epithelial cell line, VA10. It was established by transfection of primary bronchial epithelial cells with retroviral constructs containing the E6 and E7 oncogenes from HPV16. The cell line has been cultured for over 2 yr, a total of 60 passages. Although prolonged culture resulted in increased chromosomal instability, no major phenotypic drift in marker expression was observed. The cells expressed cytokeratins 5, 13, 14, and 17 suggesting a basal-like phenotype. This was further supported by the expression of alpha6beta4 integrins and the basal cell-associated transcription factor p63. The VA10 cell line generated high transepithelial electrical resistance in suspended and air-liquid interface culture, indicating functionally active tight junction (TJ) complexes. Immunocytochemistry showed the typical reticular structures of occludin and TJ-associated F-actin. VA10 produced pseudostratified layer in air-liquid interface culture with expression of p63 restricted to the basal layer. Furthermore, VA10 produced round colonies when cultured in laminin-rich reconstituted basement membrane, and immunostaining of claudin-1 and the basolateral marker beta4 integrin revealed colonies that generated polarization as expected in vivo. These data indicate that VA10 epithelia have the potential to model the bronchial epithelium in vivo and may be useful to study epithelial regeneration and repair and the effect of chemicals and potential drug candidates on TJ molecules in airway epithelia.
Description:
To access publisher full text version of this article. Please click on the hyperlink in Additional Links field
Additional Links:
http://www.springerlink.com/content/1417253101507217

Full metadata record

DC FieldValue Language
dc.contributor.authorHalldorsson, Skarphedinn-
dc.contributor.authorAsgrimsson, Valthor-
dc.contributor.authorAxelsson, Ivar-
dc.contributor.authorGudmundsson, Gudmundur Hrafn-
dc.contributor.authorSteinarsdottir, Margret-
dc.contributor.authorBaldursson, Olafur-
dc.contributor.authorGudjonsson, Thorarinn-
dc.date.accessioned2008-02-19T15:59:59Z-
dc.date.available2008-02-19T15:59:59Z-
dc.date.issued2007-10-01-
dc.date.submitted2008-02-19-
dc.identifier.citationIn Vitro Cell. Dev. Biol. Anim. 2007, 43(8-9):283-9en
dc.identifier.issn1071-2690-
dc.identifier.pmid17876679-
dc.identifier.doi10.1007/s11626-007-9050-4-
dc.identifier.urihttp://hdl.handle.net/2336/18658-
dc.descriptionTo access publisher full text version of this article. Please click on the hyperlink in Additional Links fielden
dc.description.abstractDue to the cellular complexity of the airway epithelium, it is important to carefully define bronchial cell lines that capture the phenotypic traits of a particular cell type. We describe the characterization of a human bronchial epithelial cell line, VA10. It was established by transfection of primary bronchial epithelial cells with retroviral constructs containing the E6 and E7 oncogenes from HPV16. The cell line has been cultured for over 2 yr, a total of 60 passages. Although prolonged culture resulted in increased chromosomal instability, no major phenotypic drift in marker expression was observed. The cells expressed cytokeratins 5, 13, 14, and 17 suggesting a basal-like phenotype. This was further supported by the expression of alpha6beta4 integrins and the basal cell-associated transcription factor p63. The VA10 cell line generated high transepithelial electrical resistance in suspended and air-liquid interface culture, indicating functionally active tight junction (TJ) complexes. Immunocytochemistry showed the typical reticular structures of occludin and TJ-associated F-actin. VA10 produced pseudostratified layer in air-liquid interface culture with expression of p63 restricted to the basal layer. Furthermore, VA10 produced round colonies when cultured in laminin-rich reconstituted basement membrane, and immunostaining of claudin-1 and the basolateral marker beta4 integrin revealed colonies that generated polarization as expected in vivo. These data indicate that VA10 epithelia have the potential to model the bronchial epithelium in vivo and may be useful to study epithelial regeneration and repair and the effect of chemicals and potential drug candidates on TJ molecules in airway epithelia.en
dc.language.isoenen
dc.publisherSociety for In Vitro Biologyen
dc.relation.urlhttp://www.springerlink.com/content/1417253101507217en
dc.subject.meshPubMed - in processen
dc.titleDifferentiation potential of a basal epithelial cell line established from human bronchial explant.en
dc.typeArticleen
dc.contributor.departmentBiology Institute, University of Iceland, Reykjavik, Iceland.en
dc.identifier.journalIn vitro cellular & developmental biology. Animalen

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