Positive association between DNA strand breaks in peripheral blood mononuclear cells and polyunsaturated fatty acids in red blood cells from women.

2.50
Hdl Handle:
http://hdl.handle.net/2336/19193
Title:
Positive association between DNA strand breaks in peripheral blood mononuclear cells and polyunsaturated fatty acids in red blood cells from women.
Authors:
Thorlaksdottir, Audur Y; Jonsson, Jon J; Tryggvadottir, Laufey; Skuladottir, Gudrun V; Petursdottir, Anna L; Ogmundsdottir, Helga M; Eyfjord, Jorunn E; Hardardottir, Ingibjorg
Citation:
Nutr Cancer. 2007, 59(1):21-8
Issue Date:
1-Sep-2007
Abstract:
Lipid peroxidation of polyunsaturated fatty acids (PUFA) generates reactive products that may cause DNA damage. To examine the possible relationship between DNA damage in peripheral blood mononuclear cells (PBMC) and the concentration of PUFA in red blood cells (RBC), endogenous DNA strand breaks, formamidopyrimidine DNA glycosylase (FPG) sites, and hydrogen peroxide (H2O2) sensitive sites were evaluated by the comet assay in blood samples from 98 Icelandic women. Fatty acid composition of RBC was analyzed by gas chromatography. Endogenous DNA strand breaks in PBMC correlated positively with the concentration of total PUFA, total n-3 PUFA, docosahexaenoic acid, linoleic acid, oleic acid, and palmitic acid in RBC. However, there was no association between FPG sites or H(2)O(2) sensitive sites in DNA in PBMC and the concentration of total PUFA or total saturated fatty acid in RBC. As there was no association between oxidative DNA damage or sensitivity of DNA to oxidative stress and the concentration of PUFA in RBC, the positive association between endogenous DNA strand breaks in PBMC and the concentration of total PUFA in RBC is probably not related to oxidative stress.
Description:
To access publisher full text version of this article. Please click on the hyperlink in Additional Links field
Additional Links:
http://www.informaworld.com/10.1080/01635580701365092

Full metadata record

DC FieldValue Language
dc.contributor.authorThorlaksdottir, Audur Y-
dc.contributor.authorJonsson, Jon J-
dc.contributor.authorTryggvadottir, Laufey-
dc.contributor.authorSkuladottir, Gudrun V-
dc.contributor.authorPetursdottir, Anna L-
dc.contributor.authorOgmundsdottir, Helga M-
dc.contributor.authorEyfjord, Jorunn E-
dc.contributor.authorHardardottir, Ingibjorg-
dc.date.accessioned2008-02-26T10:52:50Z-
dc.date.available2008-02-26T10:52:50Z-
dc.date.issued2007-09-01-
dc.date.submitted2008-02-26-
dc.identifier.citationNutr Cancer. 2007, 59(1):21-8en
dc.identifier.issn0163-5581-
dc.identifier.pmid17927498-
dc.identifier.doi10.1080/01635580701365092-
dc.identifier.urihttp://hdl.handle.net/2336/19193-
dc.descriptionTo access publisher full text version of this article. Please click on the hyperlink in Additional Links fielden
dc.description.abstractLipid peroxidation of polyunsaturated fatty acids (PUFA) generates reactive products that may cause DNA damage. To examine the possible relationship between DNA damage in peripheral blood mononuclear cells (PBMC) and the concentration of PUFA in red blood cells (RBC), endogenous DNA strand breaks, formamidopyrimidine DNA glycosylase (FPG) sites, and hydrogen peroxide (H2O2) sensitive sites were evaluated by the comet assay in blood samples from 98 Icelandic women. Fatty acid composition of RBC was analyzed by gas chromatography. Endogenous DNA strand breaks in PBMC correlated positively with the concentration of total PUFA, total n-3 PUFA, docosahexaenoic acid, linoleic acid, oleic acid, and palmitic acid in RBC. However, there was no association between FPG sites or H(2)O(2) sensitive sites in DNA in PBMC and the concentration of total PUFA or total saturated fatty acid in RBC. As there was no association between oxidative DNA damage or sensitivity of DNA to oxidative stress and the concentration of PUFA in RBC, the positive association between endogenous DNA strand breaks in PBMC and the concentration of total PUFA in RBC is probably not related to oxidative stress.en
dc.language.isoenen
dc.publisherLawrence Erlbaum Associatesen
dc.relation.urlhttp://www.informaworld.com/10.1080/01635580701365092en
dc.subject.meshAntioxidantsen
dc.subject.meshChromatography, Gasen
dc.subject.meshComet Assayen
dc.subject.meshDNA Breaksen
dc.subject.meshDNA Damageen
dc.subject.meshErythrocytesen
dc.subject.meshFatty Acids, Unsaturateden
dc.subject.meshFemaleen
dc.subject.meshHumansen
dc.subject.meshLeukocytes, Mononuclearen
dc.subject.meshLipid Peroxidationen
dc.subject.meshMiddle Ageden
dc.subject.meshOxidative Stressen
dc.subject.meshSmokingen
dc.titlePositive association between DNA strand breaks in peripheral blood mononuclear cells and polyunsaturated fatty acids in red blood cells from women.en
dc.typeArticleen
dc.identifier.eissn1532-7914-
dc.contributor.departmentDepartment of Biochemistry and Molecular Biology, Faculty of Medicine, University of Iceland, Reykjavik, Iceland.en
dc.identifier.journalNutrition and canceren

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