Impact of cryopreservation on serum concentration of matrix metalloproteinases (MMP)-7, TIMP-1, vascular growth factors (VEGF) and VEGF-R2 in Biobank samples.

2.50
Hdl Handle:
http://hdl.handle.net/2336/227411
Title:
Impact of cryopreservation on serum concentration of matrix metalloproteinases (MMP)-7, TIMP-1, vascular growth factors (VEGF) and VEGF-R2 in Biobank samples.
Authors:
Kisand, Kalle; Kerna, Irina; Kumm, Jaanika; Jonsson, Helgi; Tamm, Agu
Citation:
Clin. Chem. Lab. Med. 2011, 49(2):229-35
Issue Date:
Feb-2011
Abstract:
BACKGROUND: Blood biomarkers are subject to pre-analytical variability. In many cases, the stability of important new tissue biomarkers during freeze cycles and storage has not been studied sufficiently. METHODS: To test the stability of matrix metalloproteinases-7 (MMP-7) and their tissue inhibitors (TIMP-1), vascular growth factors (VEGF) and VEGF-receptor, serum samples were frozen and then thawed up to six times. The impact of storage temperature was investigated using an accelerated stability testing protocol. Stability at -20°C and -75°C was calculated using the Arrhenius equation. RESULTS: The average concentration of TIMP-1 was stable, even after six freeze/thaw cycles. One thawing did not change the concentration of MMP-7 and VEGF-receptor. However, repeated freeze/thaw cycles increased the measured values significantly. Decreases in VEGF concentrations were dramatic, even after the first freeze/thaw cycle. According to the Arrhenius calculation, MMP-7 showed excellent stability, at least 5 years at -20°C and several 100 years at -75°C. The VEGF-receptor maintains 90% of its initial concentration at -20°C over 3 months, and decades at -75°C. TIMP-1 and VEGF showed poor stability with cryopreservation, even at -75°C. CONCLUSIONS: The stability of MMP-7, TIMP-1, VEGF or VEGF-receptor in biobanking is highly variable, and this should be taken into account in the interpretation of results. A temperature -20°C is unsuitable for prolonged storage of the biomarkers investigated, and repeated thawing of sera is not recommended. VEGF is especially unstable and should be quantitated using serum that has never been frozen.
Description:
To access publisher full text version of this article. Please click on the hyperlink in Additional Links field.
Additional Links:
http://dx.doi.org/10.1515/CCLM.2011.049
Rights:
Archived with thanks to Clinical chemistry and laboratory medicine : CCLM / FESCC

Full metadata record

DC FieldValue Language
dc.contributor.authorKisand, Kalleen_GB
dc.contributor.authorKerna, Irinaen_GB
dc.contributor.authorKumm, Jaanikaen_GB
dc.contributor.authorJonsson, Helgien_GB
dc.contributor.authorTamm, Aguen_GB
dc.date.accessioned2012-06-04T10:01:11Z-
dc.date.available2012-06-04T10:01:11Z-
dc.date.issued2011-02-
dc.date.submitted2012-06-04-
dc.identifier.citationClin. Chem. Lab. Med. 2011, 49(2):229-35en_GB
dc.identifier.issn1434-6621-
dc.identifier.pmid21118050-
dc.identifier.doi10.1515/CCLM.2011.049-
dc.identifier.urihttp://hdl.handle.net/2336/227411-
dc.descriptionTo access publisher full text version of this article. Please click on the hyperlink in Additional Links field.en_GB
dc.description.abstractBACKGROUND: Blood biomarkers are subject to pre-analytical variability. In many cases, the stability of important new tissue biomarkers during freeze cycles and storage has not been studied sufficiently. METHODS: To test the stability of matrix metalloproteinases-7 (MMP-7) and their tissue inhibitors (TIMP-1), vascular growth factors (VEGF) and VEGF-receptor, serum samples were frozen and then thawed up to six times. The impact of storage temperature was investigated using an accelerated stability testing protocol. Stability at -20°C and -75°C was calculated using the Arrhenius equation. RESULTS: The average concentration of TIMP-1 was stable, even after six freeze/thaw cycles. One thawing did not change the concentration of MMP-7 and VEGF-receptor. However, repeated freeze/thaw cycles increased the measured values significantly. Decreases in VEGF concentrations were dramatic, even after the first freeze/thaw cycle. According to the Arrhenius calculation, MMP-7 showed excellent stability, at least 5 years at -20°C and several 100 years at -75°C. The VEGF-receptor maintains 90% of its initial concentration at -20°C over 3 months, and decades at -75°C. TIMP-1 and VEGF showed poor stability with cryopreservation, even at -75°C. CONCLUSIONS: The stability of MMP-7, TIMP-1, VEGF or VEGF-receptor in biobanking is highly variable, and this should be taken into account in the interpretation of results. A temperature -20°C is unsuitable for prolonged storage of the biomarkers investigated, and repeated thawing of sera is not recommended. VEGF is especially unstable and should be quantitated using serum that has never been frozen.en_GB
dc.description.sponsorshipEstonian Science Foundation EMP48en_GB
dc.language.isoenen
dc.publisherWalter De Gruyteren_GB
dc.relation.urlhttp://dx.doi.org/10.1515/CCLM.2011.049en_GB
dc.rightsArchived with thanks to Clinical chemistry and laboratory medicine : CCLM / FESCCen_GB
dc.subject.meshArtifactsen_GB
dc.subject.meshBiological Markersen_GB
dc.subject.meshBiological Specimen Banksen_GB
dc.subject.meshCryopreservationen_GB
dc.subject.meshEnzyme-Linked Immunosorbent Assayen_GB
dc.subject.meshFreezingen_GB
dc.subject.meshHumansen_GB
dc.subject.meshMatrix Metalloproteinase 7en_GB
dc.subject.meshTime Factorsen_GB
dc.subject.meshTissue Inhibitor of Metalloproteinase-1en_GB
dc.subject.meshVascular Endothelial Growth Factor Aen_GB
dc.subject.meshVascular Endothelial Growth Factor Receptor-2en_GB
dc.titleImpact of cryopreservation on serum concentration of matrix metalloproteinases (MMP)-7, TIMP-1, vascular growth factors (VEGF) and VEGF-R2 in Biobank samples.en
dc.typeArticleen
dc.contributor.departmentImmunology Group, Institute of General and Molecular Pathology, University of Tartu, Tartu, Estonia. kalle.kisand@ut.eeen_GB
dc.identifier.journalClinical chemistry and laboratory medicine : CCLM / FESCCen_GB

Related articles on PubMed

All Items in Hirsla are protected by copyright, with all rights reserved, unless otherwise indicated.