Comparison of Campylobacter jejuni isolates from human, food, veterinary and environmental sources in Iceland using PFGE, MLST and fla-SVR sequencing.

2.50
Hdl Handle:
http://hdl.handle.net/2336/227417
Title:
Comparison of Campylobacter jejuni isolates from human, food, veterinary and environmental sources in Iceland using PFGE, MLST and fla-SVR sequencing.
Authors:
Magnússon, S H; Guðmundsdóttir, S; Reynisson, E; Rúnarsson, A R; Harðardóttir, H; Gunnarson, E; Georgsson, F; Reiersen, J; Marteinsson, V Th
Citation:
J. Appl. Microbiol. 2011, 111(4):971-81
Issue Date:
Oct-2011
Abstract:
AIMS: Campylobacter jejuni isolates from various sources in Iceland were genotyped with the aim of assessing the genetic diversity, population structure, source distribution and campylobacter transmission routes to humans. METHODS AND RESULTS: A collection of 584 Campylobacter isolates were collected from clinical cases, food, animals and environment in Iceland in 1999-2002, during a period of national Campylobacter epidemic in Iceland. All isolates were characterized by pulse field gel electrophoresis (PFGE), and selected subset of 52 isolates representing the diversity of the identified PFGE types was further genotyped using multilocus sequence typing (MLST) and fla-SVR sequencing to gain better insight into the population structure. CONCLUSIONS: The results show a substantial diversity within the Icelandic Campylobacter population. Majority of the human Campylobacter infections originated from domestic chicken and cattle isolates. MLST showed the isolates to be distributed among previously reported and common sequence type complexes in the MLST database. SIGNIFICANCE AND IMPACT OF THE STUDY: The genotyping of Campylobacter from various sources has not previously been reported from Iceland, and the results of the study gave a valuable insight into the population structure of Camp. jejuni in Iceland, source distribution and transmission routes to humans. The geographical isolation of Iceland in the north Atlantic provides new information on Campylobacter population dynamics on a global scale.
Description:
To access publisher full text version of this article. Please click on the hyperlink in Additional Links field.
Additional Links:
http://dx.doi.org/10.1111/j.1365-2672.2011.05100.x
Rights:
Archived with thanks to Journal of applied microbiology

Full metadata record

DC FieldValue Language
dc.contributor.authorMagnússon, S Hen_GB
dc.contributor.authorGuðmundsdóttir, Sen_GB
dc.contributor.authorReynisson, Een_GB
dc.contributor.authorRúnarsson, A Ren_GB
dc.contributor.authorHarðardóttir, Hen_GB
dc.contributor.authorGunnarson, Een_GB
dc.contributor.authorGeorgsson, Fen_GB
dc.contributor.authorReiersen, Jen_GB
dc.contributor.authorMarteinsson, V Then_GB
dc.date.accessioned2012-06-04T11:39:25Z-
dc.date.available2012-06-04T11:39:25Z-
dc.date.issued2011-10-
dc.date.submitted2012-06-04-
dc.identifier.citationJ. Appl. Microbiol. 2011, 111(4):971-81en_GB
dc.identifier.issn1365-2672-
dc.identifier.pmid21740489-
dc.identifier.doi10.1111/j.1365-2672.2011.05100.x-
dc.identifier.urihttp://hdl.handle.net/2336/227417-
dc.descriptionTo access publisher full text version of this article. Please click on the hyperlink in Additional Links field.en_GB
dc.description.abstractAIMS: Campylobacter jejuni isolates from various sources in Iceland were genotyped with the aim of assessing the genetic diversity, population structure, source distribution and campylobacter transmission routes to humans. METHODS AND RESULTS: A collection of 584 Campylobacter isolates were collected from clinical cases, food, animals and environment in Iceland in 1999-2002, during a period of national Campylobacter epidemic in Iceland. All isolates were characterized by pulse field gel electrophoresis (PFGE), and selected subset of 52 isolates representing the diversity of the identified PFGE types was further genotyped using multilocus sequence typing (MLST) and fla-SVR sequencing to gain better insight into the population structure. CONCLUSIONS: The results show a substantial diversity within the Icelandic Campylobacter population. Majority of the human Campylobacter infections originated from domestic chicken and cattle isolates. MLST showed the isolates to be distributed among previously reported and common sequence type complexes in the MLST database. SIGNIFICANCE AND IMPACT OF THE STUDY: The genotyping of Campylobacter from various sources has not previously been reported from Iceland, and the results of the study gave a valuable insight into the population structure of Camp. jejuni in Iceland, source distribution and transmission routes to humans. The geographical isolation of Iceland in the north Atlantic provides new information on Campylobacter population dynamics on a global scale.en_GB
dc.description.sponsorshipIcelandic National Research Councilen_GB
dc.language.isoenen
dc.publisherBlackwell Scienceen_GB
dc.relation.urlhttp://dx.doi.org/10.1111/j.1365-2672.2011.05100.xen_GB
dc.rightsArchived with thanks to Journal of applied microbiologyen_GB
dc.subject.meshAnimalsen_GB
dc.subject.meshBacterial Typing Techniquesen_GB
dc.subject.meshCampylobacter Infectionsen_GB
dc.subject.meshCampylobacter jejunien_GB
dc.subject.meshCattleen_GB
dc.subject.meshChickensen_GB
dc.subject.meshElectrophoresis, Gel, Pulsed-Fielden_GB
dc.subject.meshFood Contaminationen_GB
dc.subject.meshFood Microbiologyen_GB
dc.subject.meshGastroenteritisen_GB
dc.subject.meshGenetic Variationen_GB
dc.subject.meshGenetics, Populationen_GB
dc.subject.meshGenotypeen_GB
dc.subject.meshHumansen_GB
dc.subject.meshIcelanden_GB
dc.subject.meshMeaten_GB
dc.subject.meshMultilocus Sequence Typingen_GB
dc.titleComparison of Campylobacter jejuni isolates from human, food, veterinary and environmental sources in Iceland using PFGE, MLST and fla-SVR sequencing.en
dc.typeArticleen
dc.contributor.departmentMatís ohf. Icelandic Food and Biotech R&D, Reykjavík, Iceland.en_GB
dc.identifier.journalJournal of applied microbiologyen_GB
All Items in Hirsla are protected by copyright, with all rights reserved, unless otherwise indicated.