Early life T cell responses to pneumococcal conjugates increase with age and determine the polysaccharide-specific antibody response and protective efficacy

2.50
Hdl Handle:
http://hdl.handle.net/2336/2809
Title:
Early life T cell responses to pneumococcal conjugates increase with age and determine the polysaccharide-specific antibody response and protective efficacy
Authors:
Jakobsen, Håvard; Hannesdottir, Solveig; Bjarnarson, Stefania P; Schulz, Dominique; Trannoy, Emanuelle; Siegrist, Claire-Anne; Jonsdottir, Ingileif
Citation:
Eur. J. Immunol. 2006, 36(2):287-95
Issue Date:
2006
Abstract:
Immunization with a tetanus-protein (TT) pneumococcal polysaccharide (PPS) conjugate vaccine (Pnc1-TT) induces protective immunity against lethal pneumococcal infections in neonatal and infant mice, but anti-PPS IgG response and protective efficacy is lower than in adult mice. Here, we show that reduced antibody (Ab) response and protection against infections is directly related to impaired T cell response to the carrier. Whereas spleen cells from adult mice immunized with Pnc1-TT responded with proliferation and IFN-gamma secretion to in vitro stimulation with TT, spleen cells from neonatal and infant mice did not. However, significant, but age dependent, Th2-cytokine responses were observed in mice immunized with Pnc1-TT. Impaired IFN-gamma production upon TT-stimulation in vitro was also reflected in reduced IFN-gamma/IL-5 ratio. The IL--5 response correlated with IgG anti-PPS titers, and the lack of PPS Ab in the majority of neonatal mice was clearly associated with absence of carrier-specific IL-5 production. These results show that immunization with Pnc1-TT induces carrier-specific T cell responses that increase with age and determine the levels of PPS-specific Ab elicited. Whereas a weak and Th2-biased response was observed in neonatal mice, infant mice showed a mixed Th1-Th2 response as observed in adults.
Description:
To access publisher full text version of this article. Please click on the hyperlink in Additional Links field
Additional Links:
http://dx.doi.org/10.1002/eji.200535102

Full metadata record

DC FieldValue Language
dc.contributor.authorJakobsen, Håvard-
dc.contributor.authorHannesdottir, Solveig-
dc.contributor.authorBjarnarson, Stefania P-
dc.contributor.authorSchulz, Dominique-
dc.contributor.authorTrannoy, Emanuelle-
dc.contributor.authorSiegrist, Claire-Anne-
dc.contributor.authorJonsdottir, Ingileif-
dc.date.accessioned2006-05-17T16:10:24Z-
dc.date.available2006-05-17T16:10:24Z-
dc.date.issued2006-
dc.identifier.citationEur. J. Immunol. 2006, 36(2):287-95en
dc.identifier.issn0014-2980-
dc.identifier.pmid16385627-
dc.identifier.doi10.1002/eji.200535102-
dc.identifier.otherAAI12en
dc.identifier.urihttp://hdl.handle.net/2336/2809-
dc.descriptionTo access publisher full text version of this article. Please click on the hyperlink in Additional Links fielden
dc.description.abstractImmunization with a tetanus-protein (TT) pneumococcal polysaccharide (PPS) conjugate vaccine (Pnc1-TT) induces protective immunity against lethal pneumococcal infections in neonatal and infant mice, but anti-PPS IgG response and protective efficacy is lower than in adult mice. Here, we show that reduced antibody (Ab) response and protection against infections is directly related to impaired T cell response to the carrier. Whereas spleen cells from adult mice immunized with Pnc1-TT responded with proliferation and IFN-gamma secretion to in vitro stimulation with TT, spleen cells from neonatal and infant mice did not. However, significant, but age dependent, Th2-cytokine responses were observed in mice immunized with Pnc1-TT. Impaired IFN-gamma production upon TT-stimulation in vitro was also reflected in reduced IFN-gamma/IL-5 ratio. The IL--5 response correlated with IgG anti-PPS titers, and the lack of PPS Ab in the majority of neonatal mice was clearly associated with absence of carrier-specific IL-5 production. These results show that immunization with Pnc1-TT induces carrier-specific T cell responses that increase with age and determine the levels of PPS-specific Ab elicited. Whereas a weak and Th2-biased response was observed in neonatal mice, infant mice showed a mixed Th1-Th2 response as observed in adults.en
dc.language.isoenen
dc.publisherWileyen
dc.relation.urlhttp://dx.doi.org/10.1002/eji.200535102en
dc.subjectAdjuvants, Immunologicen
dc.subjectAgingen
dc.subjectAnimalsen
dc.subjectAnimals, Newbornen
dc.subjectAntibodies, Bacterialen
dc.subjectAntibody Formationen
dc.subjectImmunologic Memoryen
dc.subjectLymphocyte Activatioen
dc.subjectMiceen
dc.subjectPneumococcal Vaccinesen
dc.subjectPolysaccharides, Bacterialen
dc.subjectTetanus Toxoiden
dc.subjectTh1 Cellsen
dc.subjectTh2 Cellsen
dc.subjectVaccines, Conjugateen
dc.titleEarly life T cell responses to pneumococcal conjugates increase with age and determine the polysaccharide-specific antibody response and protective efficacyen
dc.typeArticleen
dc.identifier.journalEuropean journal of immunologyen
dc.format.digYES-

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