Increased expression of interleukin-13 but not interleukin-4 in CD4+ cells from patients with the hyper-IgE syndrome.

2.50
Hdl Handle:
http://hdl.handle.net/2336/30574
Title:
Increased expression of interleukin-13 but not interleukin-4 in CD4+ cells from patients with the hyper-IgE syndrome.
Authors:
Gudmundsson, K O; Sigurjonsson, O E; Gudmundsson, S; Goldblatt, D; Weemaes, C M R; Haraldsson, A
Citation:
Clin. Exp. Immunol. 2002, 128(3):532-7
Issue Date:
1-Jun-2002
Abstract:
Hyper IgE syndrome (HIES) is a rare immunodeficiency disorder characterized mainly by high levels of polyclonal IgE in serum and recurrent staphylococcal abscesses of the skin and lungs. The raised IgE levels have led researchers to study the synthesis of cytokines that regulate switching of immunoglobulin production towards IgE such as interleukin-4 (IL-4), IL-12 and interferon-gamma (IFN)-gamma. However, the role of IL-13 in the disease pathogenesis has not been investigated extensively. In this study, we investigated intracellular expression of IL-4 and IL-13 in mononuclear cells and CD4+ cells isolated from patients with HIES and healthy controls. Cells were stained intracellularly with antibodies directed against IL-4 and IL-13 and analysed by flow cytometry before and after activation with PMA and calcium ionophore. The mean proportion of resting or activated IL-4 and IL-13 expressing mononuclear cells were comparable in the two groups as well as the proportion of IL-4 expressing CD4+ cells. In contrast, the mean proportion of IL-13 expressing CD4+ cells was increased significantly in patients with HIES in both the resting and the activated state compared to healthy controls. We conclude that increased expression of IL-13 in CD4+ cells from patients with HIES could account, at least partly, for raised IgE levels in those individuals.
Description:
To access publisher full text version of this article. Please click on the hyperlink in Additional Links field
Additional Links:
http://www.blackwell-synergy.com/doi/abs/10.1046/j.1365-2249.2002.01870.x; http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=1906252

Full metadata record

DC FieldValue Language
dc.contributor.authorGudmundsson, K O-
dc.contributor.authorSigurjonsson, O E-
dc.contributor.authorGudmundsson, S-
dc.contributor.authorGoldblatt, D-
dc.contributor.authorWeemaes, C M R-
dc.contributor.authorHaraldsson, A-
dc.date.accessioned2008-06-27T11:26:46Z-
dc.date.available2008-06-27T11:26:46Z-
dc.date.issued2002-06-01-
dc.date.submitted2008-06-27-
dc.identifier.citationClin. Exp. Immunol. 2002, 128(3):532-7en
dc.identifier.issn0009-9104-
dc.identifier.pmid12067309-
dc.identifier.doi10.1046/j.1365-2249.2002.01870.x.-
dc.identifier.urihttp://hdl.handle.net/2336/30574-
dc.descriptionTo access publisher full text version of this article. Please click on the hyperlink in Additional Links fielden
dc.description.abstractHyper IgE syndrome (HIES) is a rare immunodeficiency disorder characterized mainly by high levels of polyclonal IgE in serum and recurrent staphylococcal abscesses of the skin and lungs. The raised IgE levels have led researchers to study the synthesis of cytokines that regulate switching of immunoglobulin production towards IgE such as interleukin-4 (IL-4), IL-12 and interferon-gamma (IFN)-gamma. However, the role of IL-13 in the disease pathogenesis has not been investigated extensively. In this study, we investigated intracellular expression of IL-4 and IL-13 in mononuclear cells and CD4+ cells isolated from patients with HIES and healthy controls. Cells were stained intracellularly with antibodies directed against IL-4 and IL-13 and analysed by flow cytometry before and after activation with PMA and calcium ionophore. The mean proportion of resting or activated IL-4 and IL-13 expressing mononuclear cells were comparable in the two groups as well as the proportion of IL-4 expressing CD4+ cells. In contrast, the mean proportion of IL-13 expressing CD4+ cells was increased significantly in patients with HIES in both the resting and the activated state compared to healthy controls. We conclude that increased expression of IL-13 in CD4+ cells from patients with HIES could account, at least partly, for raised IgE levels in those individuals.en
dc.language.isoenen
dc.publisherBlackwell Scientific Publicationsen
dc.relation.urlhttp://www.blackwell-synergy.com/doi/abs/10.1046/j.1365-2249.2002.01870.xen
dc.relation.urlhttp://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=1906252en
dc.subject.meshCD4-Positive T-Lymphocytesen
dc.subject.meshCells, Cultureden
dc.subject.meshFemaleen
dc.subject.meshHumansen
dc.subject.meshInterleukin-13en
dc.subject.meshInterleukin-4en
dc.subject.meshJob's Syndromeen
dc.subject.meshLeukocytes, Mononuclearen
dc.subject.meshMaleen
dc.titleIncreased expression of interleukin-13 but not interleukin-4 in CD4+ cells from patients with the hyper-IgE syndrome.en
dc.typeArticleen
dc.contributor.departmentThe Blood Bank, Landspitali-University Hospital, Reykjavik, Iceland. kristbj@landspitali.isen
dc.identifier.journalClinical and experimental immunologyen

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