Characterization of the antibody response to pneumococcal glycoconjugates and the effect of heat-labile enterotoxin on IGg subclasses after intranasal immunization.

2.50
Hdl Handle:
http://hdl.handle.net/2336/33613
Title:
Characterization of the antibody response to pneumococcal glycoconjugates and the effect of heat-labile enterotoxin on IGg subclasses after intranasal immunization.
Authors:
Jakobsen, H; Adarna, BC; Schulz, D; Rappuoli, R; Jonsdottir, I
Citation:
J. Infect. Dis. 2001, 183(10):1494-500
Issue Date:
15-May-2001
Abstract:
The antibody response to pneumococcal glycoconjugate (Pnc) was characterized by analyzing pneumococcal polysaccharide (PPS)-- and protein carrier-specific IgG subclass profiles and their relationship. Mice were immunized intranasally (inl) or subcutaneously (sc) with Pnc with mutants of Escherichia coli heat-labile enterotoxin, LT-R72 and LT-K63, as mucosal adjuvants. Subcutaneous immunization with Pnc alone induced predominantly IgG1, whereas native PPS administered sc induced very low IgG titers that were exclusively of the IgG3 subclass. Compared with sc immunization with Pnc alone, inl immunization with Pnc and LT mutants induced significantly higher systemic IgG2a, IgG3, and IgA antibodies to both PPS and the carrier, whereas the IgG1 titers were comparable. There also was a significant correlation between PPS- and protein carrier--specific antibody responses for all IgG subclasses. This demonstrates that LT mutants can be used to both enhance and modulate the antibody response to the PS moiety of glycoconjugate vaccines.
Description:
To access publisher full text version of this article. Please click on the hyperlink in Additional Links field
Additional Links:
http://www.journals.uchicago.edu/doi/abs/10.1086/320202

Full metadata record

DC FieldValue Language
dc.contributor.authorJakobsen, H-
dc.contributor.authorAdarna, BC-
dc.contributor.authorSchulz, D-
dc.contributor.authorRappuoli, R-
dc.contributor.authorJonsdottir, I-
dc.date.accessioned2008-07-29T15:26:54Z-
dc.date.available2008-07-29T15:26:54Z-
dc.date.issued2001-05-15-
dc.date.submitted2008-07-29-
dc.identifier.citationJ. Infect. Dis. 2001, 183(10):1494-500en
dc.identifier.issn0022-1899-
dc.identifier.pmid11319685-
dc.identifier.doi10.1086/320202-
dc.identifier.urihttp://hdl.handle.net/2336/33613-
dc.descriptionTo access publisher full text version of this article. Please click on the hyperlink in Additional Links fielden
dc.description.abstractThe antibody response to pneumococcal glycoconjugate (Pnc) was characterized by analyzing pneumococcal polysaccharide (PPS)-- and protein carrier-specific IgG subclass profiles and their relationship. Mice were immunized intranasally (inl) or subcutaneously (sc) with Pnc with mutants of Escherichia coli heat-labile enterotoxin, LT-R72 and LT-K63, as mucosal adjuvants. Subcutaneous immunization with Pnc alone induced predominantly IgG1, whereas native PPS administered sc induced very low IgG titers that were exclusively of the IgG3 subclass. Compared with sc immunization with Pnc alone, inl immunization with Pnc and LT mutants induced significantly higher systemic IgG2a, IgG3, and IgA antibodies to both PPS and the carrier, whereas the IgG1 titers were comparable. There also was a significant correlation between PPS- and protein carrier--specific antibody responses for all IgG subclasses. This demonstrates that LT mutants can be used to both enhance and modulate the antibody response to the PS moiety of glycoconjugate vaccines.en
dc.language.isoenen
dc.publisherUniversity of Chicago Pressen
dc.relation.urlhttp://www.journals.uchicago.edu/doi/abs/10.1086/320202en
dc.subject.meshAdjuvants, Immunologicen
dc.subject.meshAdministration, Intranasalen
dc.subject.meshAnimalsen
dc.subject.meshAntibodies, Bacterialen
dc.subject.meshBacterial Toxinsen
dc.subject.meshEnterotoxinsen
dc.subject.meshEscherichia coli Proteinsen
dc.subject.meshFemaleen
dc.subject.meshGlycoconjugatesen
dc.subject.meshImmunoglobulin Gen
dc.subject.meshMiceen
dc.subject.meshMutationen
dc.subject.meshPneumococcal Vaccinesen
dc.subject.meshPolysaccharides, Bacterialen
dc.subject.meshStreptococcus pneumoniaeen
dc.subject.meshTetanus Toxoiden
dc.titleCharacterization of the antibody response to pneumococcal glycoconjugates and the effect of heat-labile enterotoxin on IGg subclasses after intranasal immunization.en
dc.typeArticleen
dc.contributor.departmentDepartment of Immunology, Landspitali-University Hospital, Reykjavik, Iceland.en
dc.identifier.journalJournal of infectious diseasesen
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