Cartilage oligomeric matrix protein increases in serum after the start of growth hormone treatment in prepubertal children

2.50
Hdl Handle:
http://hdl.handle.net/2336/3420
Title:
Cartilage oligomeric matrix protein increases in serum after the start of growth hormone treatment in prepubertal children
Authors:
Bjarnason, R; Andersson, B; Kim, H S; Olsson, B; Swolin-Eide, D; Wickelgren, R; Kriström, B; Carlsson, B; Albertsson-Wikland, K; Carlsson, L M S
Citation:
J. Clin. Endocrinol. Metab. 2004, 89(10):5156-60
Issue Date:
2004
Abstract:
Both GH and IGF-I stimulate bone growth, but the molecular mechanisms mediating their effects on the growth plate are not fully understood. We measured gene expression by microarray analysis in primary cultured human chondrocytes treated with either GH or IGF-I. One of the genes found to be up-regulated by both GH and IGF-I was that encoding cartilage oligomeric matrix protein (COMP). This protein is predominantly found in the extracellular matrix of cartilage. Mutations in the COMP gene have been associated with syndromes of short stature. To verify that COMP is regulated by GH in vivo, we measured COMP levels in serum in short children treated with GH. The study included 113 short prepubertal children (14 girls and 99 boys) with a mean (+/- sd) age of 8.84 +/- 2.76 yr, height sd score of -2.74 +/- 0.67, and IGF-I sd score of -1.21 +/- 1.07 at the start of GH administration. Serum levels of COMP were 1.58 +/- 0.28, 1.83 +/- 0.28 (P < 0.0001), 1.91 +/- 0.28 (P < 0.0001), 1.78 +/- 0.28 (P < 0.001), and 1.70 +/- 0.24 (P < 0.05) microg/ml at baseline and after 1 wk and 1, 3, and 12 months, respectively.In conclusion, we have demonstrated that COMP expression is up-regulated by both GH and IGF-I in primary cultured human chondrocytes. Furthermore, serum levels of COMP increase after the start of GH treatment in short children.
Description:
To access publisher full text version of this article. Please click on the hyperlink in Additional Links field
Additional Links:
http://jcem.endojournals.org/cgi/content/full/89/10/5156

Full metadata record

DC FieldValue Language
dc.contributor.authorBjarnason, R-
dc.contributor.authorAndersson, B-
dc.contributor.authorKim, H S-
dc.contributor.authorOlsson, B-
dc.contributor.authorSwolin-Eide, D-
dc.contributor.authorWickelgren, R-
dc.contributor.authorKriström, B-
dc.contributor.authorCarlsson, B-
dc.contributor.authorAlbertsson-Wikland, K-
dc.contributor.authorCarlsson, L M S-
dc.date.accessioned2006-07-12T11:20:53Z-
dc.date.available2006-07-12T11:20:53Z-
dc.date.issued2004-
dc.identifier.citationJ. Clin. Endocrinol. Metab. 2004, 89(10):5156-60en
dc.identifier.issn0021-972X-
dc.identifier.pmid15472220-
dc.identifier.doi10.1210/jc.2004-0587-
dc.identifier.otherPED12en
dc.identifier.urihttp://hdl.handle.net/2336/3420-
dc.descriptionTo access publisher full text version of this article. Please click on the hyperlink in Additional Links fielden
dc.description.abstractBoth GH and IGF-I stimulate bone growth, but the molecular mechanisms mediating their effects on the growth plate are not fully understood. We measured gene expression by microarray analysis in primary cultured human chondrocytes treated with either GH or IGF-I. One of the genes found to be up-regulated by both GH and IGF-I was that encoding cartilage oligomeric matrix protein (COMP). This protein is predominantly found in the extracellular matrix of cartilage. Mutations in the COMP gene have been associated with syndromes of short stature. To verify that COMP is regulated by GH in vivo, we measured COMP levels in serum in short children treated with GH. The study included 113 short prepubertal children (14 girls and 99 boys) with a mean (+/- sd) age of 8.84 +/- 2.76 yr, height sd score of -2.74 +/- 0.67, and IGF-I sd score of -1.21 +/- 1.07 at the start of GH administration. Serum levels of COMP were 1.58 +/- 0.28, 1.83 +/- 0.28 (P < 0.0001), 1.91 +/- 0.28 (P < 0.0001), 1.78 +/- 0.28 (P < 0.001), and 1.70 +/- 0.24 (P < 0.05) microg/ml at baseline and after 1 wk and 1, 3, and 12 months, respectively.In conclusion, we have demonstrated that COMP expression is up-regulated by both GH and IGF-I in primary cultured human chondrocytes. Furthermore, serum levels of COMP increase after the start of GH treatment in short children.en
dc.language.isoenen
dc.publisherEndocrine Societyen
dc.relation.urlhttp://jcem.endojournals.org/cgi/content/full/89/10/5156en
dc.subjectBody Heighten
dc.subjectCells, Cultureden
dc.subjectChilden
dc.subjectChild, Preschoolen
dc.subjectChondrocytesen
dc.subjectExtracellular Matrix Proteinsen
dc.subjectFemaleen
dc.subjectGene Expressionen
dc.subjectGlycoproteinsen
dc.subjectGrowth Disordersen
dc.subjectHuman Growth Hormoneen
dc.subjectHumansen
dc.subjectInfanten
dc.subjectInsulin-Like Growth Factor Ien
dc.subjectMaleen
dc.subjectOligonucleotide Array Sequence Analysisen
dc.subjectResearch Support, Non-U.S. Gov'ten
dc.titleCartilage oligomeric matrix protein increases in serum after the start of growth hormone treatment in prepubertal childrenen
dc.typeArticleen
dc.identifier.journalJournal of clinical endocrinology and metabolismen
dc.format.digYES-

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