Intracellular Fas ligand in normal and malignant breast epithelium does not induce apoptosis in Fas-sensitive cells.

2.50
Hdl Handle:
http://hdl.handle.net/2336/34252
Title:
Intracellular Fas ligand in normal and malignant breast epithelium does not induce apoptosis in Fas-sensitive cells.
Authors:
Ragnarsson, G B; Mikaelsdottir, E K; Vidarsson, H; Jonasson, J G; Olafsdottir, K; Kristjansdottir, K; Kjartansson, J; Ogmundsdóttir, H M; Rafnar, T
Citation:
Br. J. Cancer. 2000, 83(12):1715-21
Issue Date:
1-Dec-2000
Abstract:
Fas ligand (FasL) is expressed on some cancers and may play a role in the immune evasion of the tumour. We used immuno-histochemistry to study the expression of Fas and FasL in tissue samples from breast cancer patients, as well as normal breast tissue. Our results show that Fas and FasL are co-expressed both in normal tissue and in breast tumours. Fas and FasL mRNA were expressed in fresh normal and malignant breast tissue, as well as cultured breast epithelium and breast cancer cell lines. Flow cytometry analysis of live cells failed to detect FasL on the surface of normal or malignant breast cells; however, both stained positive for FasL after permeabilization. Fas was detected on the surface of normal breast cells and T47D and MCF-10A cell lines but only intracellularly in other breast cell lines tested. Neither normal breast epithelium nor breast cell lines induced Fas-dependent apoptosis in Jurkat cells. Finally, 20 tumour samples were stained for apoptosis. Few apoptotic cells were detected and there was no increase in apoptotic cells on the borders between tumour cells and lymphocytes. We conclude that FasL is expressed intracellularly in both normal and malignant breast epithelium and unlikely to be important for the immune evasion of breast tumours.
Description:
To access publisher full text version of this article. Please click on the hyperlink in Additional Links field
Additional Links:
http://dx.doi.org/10.1054/bjoc.2000.1506

Full metadata record

DC FieldValue Language
dc.contributor.authorRagnarsson, G B-
dc.contributor.authorMikaelsdottir, E K-
dc.contributor.authorVidarsson, H-
dc.contributor.authorJonasson, J G-
dc.contributor.authorOlafsdottir, K-
dc.contributor.authorKristjansdottir, K-
dc.contributor.authorKjartansson, J-
dc.contributor.authorOgmundsdóttir, H M-
dc.contributor.authorRafnar, T-
dc.date.accessioned2008-08-05T13:19:23Z-
dc.date.available2008-08-05T13:19:23Z-
dc.date.issued2000-12-01-
dc.date.submitted2008-08-05-
dc.identifier.citationBr. J. Cancer. 2000, 83(12):1715-21en
dc.identifier.issn0007-0920-
dc.identifier.pmid11104571-
dc.identifier.doi10.1054/bjoc.2000.1506-
dc.identifier.urihttp://hdl.handle.net/2336/34252-
dc.descriptionTo access publisher full text version of this article. Please click on the hyperlink in Additional Links fielden
dc.description.abstractFas ligand (FasL) is expressed on some cancers and may play a role in the immune evasion of the tumour. We used immuno-histochemistry to study the expression of Fas and FasL in tissue samples from breast cancer patients, as well as normal breast tissue. Our results show that Fas and FasL are co-expressed both in normal tissue and in breast tumours. Fas and FasL mRNA were expressed in fresh normal and malignant breast tissue, as well as cultured breast epithelium and breast cancer cell lines. Flow cytometry analysis of live cells failed to detect FasL on the surface of normal or malignant breast cells; however, both stained positive for FasL after permeabilization. Fas was detected on the surface of normal breast cells and T47D and MCF-10A cell lines but only intracellularly in other breast cell lines tested. Neither normal breast epithelium nor breast cell lines induced Fas-dependent apoptosis in Jurkat cells. Finally, 20 tumour samples were stained for apoptosis. Few apoptotic cells were detected and there was no increase in apoptotic cells on the borders between tumour cells and lymphocytes. We conclude that FasL is expressed intracellularly in both normal and malignant breast epithelium and unlikely to be important for the immune evasion of breast tumours.en
dc.language.isoenen
dc.publisherNature Publishing Group on behalf of Cancer Research UKen
dc.relation.urlhttp://dx.doi.org/10.1054/bjoc.2000.1506en
dc.subject.meshAnimalsen
dc.subject.meshAntigens, CD95en
dc.subject.meshApoptosisen
dc.subject.meshBlotting, Westernen
dc.subject.meshBreasten
dc.subject.meshBreast Neoplasmsen
dc.subject.meshCells, Cultureden
dc.subject.meshEpitheliumen
dc.subject.meshFas Ligand Proteinen
dc.subject.meshFemaleen
dc.subject.meshFlow Cytometryen
dc.subject.meshGene Expression Regulationen
dc.subject.meshHumansen
dc.subject.meshImmunohistochemistryen
dc.subject.meshJurkat Cellsen
dc.subject.meshMembrane Glycoproteinsen
dc.subject.meshRNAen
dc.subject.meshReverse Transcriptase Polymerase Chain Reactionen
dc.subject.meshTumor Cells, Cultureden
dc.titleIntracellular Fas ligand in normal and malignant breast epithelium does not induce apoptosis in Fas-sensitive cells.en
dc.typeArticleen
dc.contributor.departmentMolecular and Cell Biology Research Laboratory, Icelandic Cancer Society, P.O. Box 5420, Reykjavík, IS-125.en
dc.identifier.journalBritish journal of canceren

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