Pneumococcal pneumonia and bacteremia model in mice for the analysis of protective antibodies

2.50
Hdl Handle:
http://hdl.handle.net/2336/34475
Title:
Pneumococcal pneumonia and bacteremia model in mice for the analysis of protective antibodies
Authors:
Saeland, E; Vidarsson, G; Jonsdottir, I
Citation:
Microb. Pathog. 2000, 29(2):81-91
Issue Date:
1-Aug-2000
Abstract:
Pneumococci cause infection by colonizing the nasopharynx and invading the mucosal surfaces. Infection models in mice, where the natural route of infection is mimicked, may be useful to study antibody mediated protection against pneumococcal pneumonia and bacteremia. We have established a pneumococcal pneumonia and bacteremia model in mice and investigated the protective capacity of human antibodies. Intranasal challenge with serotypes 1, 3, 6A and 8 caused lung infection and bacteremia which was lethal. Serotype 6B caused low, but detectable, infection and other serotypes tested were not virulent. Passive immunization with a human IgG preparation i.p. protected mice in a dose dependent manner against bacteremia caused by the virulent serotypes (except serotype 3) and partially or completely cleared pneumococci from the lungs of mice infected with serotypes 1, 6A and 8. Adsorption of antibodies with homologous capsular polysaccharides eliminated protection against disease but adsorption with cell wall polysaccharides (CWPS) did not. Furthermore, a good correlation was observed between protection of sera in vivo and opsonic activity in vitro. The results indicate that the model may be useful to analyse the levels, isotypes, specificity and other characteristics of human antibodies which protect against pneumococcal infection and to evaluate the protective potential of pneumococcal vaccine candidates.
Description:
To access publisher full text version of this article. Please click on the hyperlink in Additional Links field
Additional Links:
http://www.sciencedirect.com/science/article/B6WN6-45C0RH1-11/2/565707e9e4dc849776f93f54fc6aade6

Full metadata record

DC FieldValue Language
dc.contributor.authorSaeland, E-
dc.contributor.authorVidarsson, G-
dc.contributor.authorJonsdottir, I-
dc.date.accessioned2008-08-06T09:40:45Z-
dc.date.available2008-08-06T09:40:45Z-
dc.date.issued2000-08-01-
dc.date.submitted2008-08-06-
dc.identifier.citationMicrob. Pathog. 2000, 29(2):81-91en
dc.identifier.issn0882-4010-
dc.identifier.pmid10906263-
dc.identifier.doi10.1006/mpat.2000.0363-
dc.identifier.urihttp://hdl.handle.net/2336/34475-
dc.descriptionTo access publisher full text version of this article. Please click on the hyperlink in Additional Links fielden
dc.description.abstractPneumococci cause infection by colonizing the nasopharynx and invading the mucosal surfaces. Infection models in mice, where the natural route of infection is mimicked, may be useful to study antibody mediated protection against pneumococcal pneumonia and bacteremia. We have established a pneumococcal pneumonia and bacteremia model in mice and investigated the protective capacity of human antibodies. Intranasal challenge with serotypes 1, 3, 6A and 8 caused lung infection and bacteremia which was lethal. Serotype 6B caused low, but detectable, infection and other serotypes tested were not virulent. Passive immunization with a human IgG preparation i.p. protected mice in a dose dependent manner against bacteremia caused by the virulent serotypes (except serotype 3) and partially or completely cleared pneumococci from the lungs of mice infected with serotypes 1, 6A and 8. Adsorption of antibodies with homologous capsular polysaccharides eliminated protection against disease but adsorption with cell wall polysaccharides (CWPS) did not. Furthermore, a good correlation was observed between protection of sera in vivo and opsonic activity in vitro. The results indicate that the model may be useful to analyse the levels, isotypes, specificity and other characteristics of human antibodies which protect against pneumococcal infection and to evaluate the protective potential of pneumococcal vaccine candidates.en
dc.language.isoenen
dc.publisherAcademic Pressen
dc.relation.urlhttp://www.sciencedirect.com/science/article/B6WN6-45C0RH1-11/2/565707e9e4dc849776f93f54fc6aade6en
dc.subject.meshAnimalsen
dc.subject.meshAntibodies, Bacterialen
dc.subject.meshBacteremiaen
dc.subject.meshColony Count, Microbialen
dc.subject.meshDisease Models, Animalen
dc.subject.meshFemaleen
dc.subject.meshHumansen
dc.subject.meshImmunization, Passiveen
dc.subject.meshMaleen
dc.subject.meshMiceen
dc.subject.meshPneumonia, Pneumococcalen
dc.subject.meshStreptococcus pneumoniaeen
dc.subject.meshVirulenceen
dc.titlePneumococcal pneumonia and bacteremia model in mice for the analysis of protective antibodiesen
dc.typeArticleen
dc.contributor.departmentNational University Hospital, Department of Immunology, Reykjavik, Icelanden
dc.identifier.journalMicrobial pathogenesisen

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