P53 mutations analysis in benign and malignant breast lesions: using needle rinses from fine-needle aspirations

2.50
Hdl Handle:
http://hdl.handle.net/2336/34873
Title:
P53 mutations analysis in benign and malignant breast lesions: using needle rinses from fine-needle aspirations
Authors:
Gudlaugsdottir, S; Sigurdardottir, V; Snorradottir, M; Jonasson, J G; Ogmundsdottir, H; Eyfjord, J E
Citation:
Diagn. Cytopathol. 2000, 22(5):268-74
Issue Date:
1-May-2000
Abstract:
The fine-needle aspiration (FNA) technique is a widely used method for diagnostic assessment of breast diseases. In the current study we investigated the feasibility of sampling material for genetic studies from the same FNA samples as would be used for breast cytology. After making smears for cytological examination, the needle was rinsed into phosphate-buffered saline (PBS) solution. The material gained was sufficient for a polymerase chain reaction (PCR)-based study. As the FNA samples reflect a broad range of breast diseases, it is possible to study genetic changes at various stages of the neoplastic process. We looked for mutations in the p53 tumor suppressor gene in 198 FNA needle rinses, 42 from carcinomas and 156 from cytologically benign lesions. In the malignant samples, 22% carried mutations in the p53 gene. We also looked for p53 mutations in matching tissue sections from tumors and found the FNA needle rinses to represent the tumor well. In addition, three mutations in cytologically benign lesions were found, but none of these 3 patients were diagnosed with malignant tumors in the time frame of the study. The clinical significance of p53 mutations in benign breast tissue remains to be determined.
Description:
To access publisher full text version of this article. Please click on the hyperlink in Additional Links field
Additional Links:
http://dx.doi.org/10.1002/(SICI)1097-0339(200005)22:5<268::AID-DC2>3.0.CO;2-X

Full metadata record

DC FieldValue Language
dc.contributor.authorGudlaugsdottir, S-
dc.contributor.authorSigurdardottir, V-
dc.contributor.authorSnorradottir, M-
dc.contributor.authorJonasson, J G-
dc.contributor.authorOgmundsdottir, H-
dc.contributor.authorEyfjord, J E-
dc.date.accessioned2008-08-08T13:19:44Z-
dc.date.available2008-08-08T13:19:44Z-
dc.date.issued2000-05-01-
dc.date.submitted2008-08-08-
dc.identifier.citationDiagn. Cytopathol. 2000, 22(5):268-74en
dc.identifier.issn8755-1039-
dc.identifier.pmid10790231-
dc.identifier.doi10.1002/(SICI)1097-0339(200005)22:5<268::AID-DC2>3.0.CO;2-X-
dc.identifier.urihttp://hdl.handle.net/2336/34873-
dc.descriptionTo access publisher full text version of this article. Please click on the hyperlink in Additional Links fielden
dc.description.abstractThe fine-needle aspiration (FNA) technique is a widely used method for diagnostic assessment of breast diseases. In the current study we investigated the feasibility of sampling material for genetic studies from the same FNA samples as would be used for breast cytology. After making smears for cytological examination, the needle was rinsed into phosphate-buffered saline (PBS) solution. The material gained was sufficient for a polymerase chain reaction (PCR)-based study. As the FNA samples reflect a broad range of breast diseases, it is possible to study genetic changes at various stages of the neoplastic process. We looked for mutations in the p53 tumor suppressor gene in 198 FNA needle rinses, 42 from carcinomas and 156 from cytologically benign lesions. In the malignant samples, 22% carried mutations in the p53 gene. We also looked for p53 mutations in matching tissue sections from tumors and found the FNA needle rinses to represent the tumor well. In addition, three mutations in cytologically benign lesions were found, but none of these 3 patients were diagnosed with malignant tumors in the time frame of the study. The clinical significance of p53 mutations in benign breast tissue remains to be determined.en
dc.language.isoenen
dc.publisherWiley-Lissen
dc.relation.urlhttp://dx.doi.org/10.1002/(SICI)1097-0339(200005)22:5<268::AID-DC2>3.0.CO;2-Xen
dc.subject.meshBiopsy, Needleen
dc.subject.meshBreast Neoplasmsen
dc.subject.meshDNA Mutational Analysisen
dc.subject.meshFemaleen
dc.subject.meshGenes, p53en
dc.subject.meshHumansen
dc.subject.meshMutationen
dc.titleP53 mutations analysis in benign and malignant breast lesions: using needle rinses from fine-needle aspirationsen
dc.typeArticleen
dc.contributor.departmentMolecular and Cell Biology Research Laboratory, Icelandic Cancer Society, Reykjavik, Iceland.en
dc.identifier.journalDiagnostic cytopathologyen
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