Neonatal immune response and serum bactericidal activity induced by a meningococcal conjugate vaccine is enhanced by LT-K63 and CpG2006

2.50
Hdl Handle:
http://hdl.handle.net/2336/35152
Title:
Neonatal immune response and serum bactericidal activity induced by a meningococcal conjugate vaccine is enhanced by LT-K63 and CpG2006
Authors:
Brynjolfsson, Siggeir F; Bjarnarson, Stefania P; Mori, Elena; Del Giudice, Giuseppe; Jonsdottir, Ingileif
Citation:
Vaccine 2008, 26(35):4557-62
Issue Date:
18-Aug-2008
Abstract:
Neonates have a poorly developed immune system. Therefore it is important to develop vaccination strategies that induce protective immunity and immunological memory against pathogens early in life. The immunogenicity of a meningococcal serogroup C polysaccharide conjugate (MenC-CRM(197)) was assessed in neonatal mice, and effects of LT-K63 and CpG2006 and immunisation routes were compared. Neonatal mice were primed subcutaneously (s.c.) or intranasally (i.n.) with MenC-CRM(197) with or without LT-K63 or CpG2006 and re-immunised 16 and 30 days later by the same route and formulation. Antibody levels were measured and generation of immunological memory assessed by affinity maturation and kinetics of the Ab response. Serum bactericidal activity (SBA) was measured to evaluate protective efficacy. The second and third dose of MenC-CRM(197) mixed with either LT-K63 or CpG2006 induced a rapid increase in MenC-specific IgG antibodies, to levels higher than elicited by MenC-CRM(197) alone (P<0.01) and in unimmunised mice (P<0.001), indicating efficient generation of memory by priming through both s.c. and i.n. routes. SBA was detected after three s.c. immunisations with MenC-CRM(197) s.c. alone. However, only two doses of MenC-CRM(197)+LT-K63 or MenC-CRM(197)+CpG2006 were needed to induce SBA levels>16. LT-K63 and CpG2006 enhanced neonatal antibody responses, affinity maturation, immunological memory to the conjugate MenC-CRM(197) and protective immunity. These results encourage the development of neonatal vaccination strategies to induce protective immunity and immunological memory against meningococcal disease.
Description:
To access publisher full text version of this article. Please click on the hyperlink in Additional Links field
Additional Links:
http://www.sciencedirect.com/science/article/B6TD4-4SSG6XF-4/2/f3273a92b2ce42c18bb01fb496f5a8b3

Full metadata record

DC FieldValue Language
dc.contributor.authorBrynjolfsson, Siggeir F-
dc.contributor.authorBjarnarson, Stefania P-
dc.contributor.authorMori, Elena-
dc.contributor.authorDel Giudice, Giuseppe-
dc.contributor.authorJonsdottir, Ingileif-
dc.date.accessioned2008-08-11T16:56:46Z-
dc.date.available2008-08-11T16:56:46Z-
dc.date.issued2008-08-18-
dc.date.submitted2008-09-11-
dc.identifier.citationVaccine 2008, 26(35):4557-62en
dc.identifier.issn0264-410X-
dc.identifier.pmid18597905-
dc.identifier.doi10.1016/j.vaccine.2008.05.083-
dc.identifier.urihttp://hdl.handle.net/2336/35152-
dc.descriptionTo access publisher full text version of this article. Please click on the hyperlink in Additional Links fielden
dc.description.abstractNeonates have a poorly developed immune system. Therefore it is important to develop vaccination strategies that induce protective immunity and immunological memory against pathogens early in life. The immunogenicity of a meningococcal serogroup C polysaccharide conjugate (MenC-CRM(197)) was assessed in neonatal mice, and effects of LT-K63 and CpG2006 and immunisation routes were compared. Neonatal mice were primed subcutaneously (s.c.) or intranasally (i.n.) with MenC-CRM(197) with or without LT-K63 or CpG2006 and re-immunised 16 and 30 days later by the same route and formulation. Antibody levels were measured and generation of immunological memory assessed by affinity maturation and kinetics of the Ab response. Serum bactericidal activity (SBA) was measured to evaluate protective efficacy. The second and third dose of MenC-CRM(197) mixed with either LT-K63 or CpG2006 induced a rapid increase in MenC-specific IgG antibodies, to levels higher than elicited by MenC-CRM(197) alone (P<0.01) and in unimmunised mice (P<0.001), indicating efficient generation of memory by priming through both s.c. and i.n. routes. SBA was detected after three s.c. immunisations with MenC-CRM(197) s.c. alone. However, only two doses of MenC-CRM(197)+LT-K63 or MenC-CRM(197)+CpG2006 were needed to induce SBA levels>16. LT-K63 and CpG2006 enhanced neonatal antibody responses, affinity maturation, immunological memory to the conjugate MenC-CRM(197) and protective immunity. These results encourage the development of neonatal vaccination strategies to induce protective immunity and immunological memory against meningococcal disease.en
dc.language.isoenen
dc.publisherElsevier Scienceen
dc.relation.urlhttp://www.sciencedirect.com/science/article/B6TD4-4SSG6XF-4/2/f3273a92b2ce42c18bb01fb496f5a8b3en
dc.subject.meshMeningococcal Vaccinesen
dc.subject.meshAntibodies, Bacterialen
dc.subject.meshBlood Bactericidal Activityen
dc.subject.meshMicrobial Viabilityen
dc.subject.meshEscherichia coli Proteinsen
dc.titleNeonatal immune response and serum bactericidal activity induced by a meningococcal conjugate vaccine is enhanced by LT-K63 and CpG2006en
dc.typeArticleen
dc.contributor.departmentLandspitali, Department of Immunology, Reykjavik, Iceland; University of Iceland, Faculty of Medicine, Reykjavik, Iceland.en
dc.identifier.journalVaccineen

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