Adjuvants LT-K63 and CpG enhance the activation of dendritic cells in neonatal mice

2.50
Hdl Handle:
http://hdl.handle.net/2336/41715
Title:
Adjuvants LT-K63 and CpG enhance the activation of dendritic cells in neonatal mice
Authors:
Hannesdottir, S G; Olafsdottir, T A; Giudice, G D; Jonsdottir, I
Citation:
Scand. J. Immunol. 2008, 68(5):469-75
Issue Date:
1-Nov-2008
Abstract:
Dendritic cells (DC) play a major role in the priming of T cells and initiating specific immune responses. We assessed the effects of the adjuvants LT-K63 and CpG on neonatal DC in vivo and in vitro. Cytokine levels (IL-10, IL-12p70 and IL-12p40/IL-23p40) were measured and the expression of the activation markers CD86, CD40 and MHCII on CD11c+ DC was analysed by using FACS. The proportion of MHCII high CD11c+ DC was higher in neonatal mice immunized with a pneumococcal conjugate (PncTT) and LT-K63 or CpG compared with that when PncTT was alone. In vitro stimulation with LT-K63 enhanced the expression of CD86 more on CD11c+ DC from spleens of mice immunized as neonates than those immunized as adults, whereas in vitro stimulation with CpG enhanced the expression of CD86 and CD40 on CD11c+ DC similarly in both age groups. CpG stimulation in vitro enhanced IL-10 and IL-12(p70) production in mice immunized as neonates with PncTT and either adjuvant, but not PncTT alone. The adjuvants LT-K63 and CpG enhance the activation of CD11c+ DC in mice immunized as neonates and can thereby overcome one of the limiting factors in the initiation of the immune response to conjugate vaccines in early life. The fact that neonatal DC are more susceptible to stimulation with either adjuvant, LT-K63 or CpG, could imply that neonatal CD11c+ DC are more easily activated than adult CD11c+ DC, and /or be a consequence of the predominance of different DC subsets in neonatal and adult mice.
Description:
To access publisher full text version of this article. Please click on the hyperlink in Additional Links field
Additional Links:
http://dx.doi.org/10.1111/j.1365-3083.2008.02165.x

Full metadata record

DC FieldValue Language
dc.contributor.authorHannesdottir, S G-
dc.contributor.authorOlafsdottir, T A-
dc.contributor.authorGiudice, G D-
dc.contributor.authorJonsdottir, I-
dc.date.accessioned2008-12-02T11:44:39Z-
dc.date.available2008-12-02T11:44:39Z-
dc.date.issued2008-11-01-
dc.date.submitted2008-12-02-
dc.identifier.citationScand. J. Immunol. 2008, 68(5):469-75en
dc.identifier.issn1365-3083-
dc.identifier.pmid18946928-
dc.identifier.doi10.1111/j.1365-3083.2008.02165.x-
dc.identifier.urihttp://hdl.handle.net/2336/41715-
dc.descriptionTo access publisher full text version of this article. Please click on the hyperlink in Additional Links fielden
dc.description.abstractDendritic cells (DC) play a major role in the priming of T cells and initiating specific immune responses. We assessed the effects of the adjuvants LT-K63 and CpG on neonatal DC in vivo and in vitro. Cytokine levels (IL-10, IL-12p70 and IL-12p40/IL-23p40) were measured and the expression of the activation markers CD86, CD40 and MHCII on CD11c+ DC was analysed by using FACS. The proportion of MHCII high CD11c+ DC was higher in neonatal mice immunized with a pneumococcal conjugate (PncTT) and LT-K63 or CpG compared with that when PncTT was alone. In vitro stimulation with LT-K63 enhanced the expression of CD86 more on CD11c+ DC from spleens of mice immunized as neonates than those immunized as adults, whereas in vitro stimulation with CpG enhanced the expression of CD86 and CD40 on CD11c+ DC similarly in both age groups. CpG stimulation in vitro enhanced IL-10 and IL-12(p70) production in mice immunized as neonates with PncTT and either adjuvant, but not PncTT alone. The adjuvants LT-K63 and CpG enhance the activation of CD11c+ DC in mice immunized as neonates and can thereby overcome one of the limiting factors in the initiation of the immune response to conjugate vaccines in early life. The fact that neonatal DC are more susceptible to stimulation with either adjuvant, LT-K63 or CpG, could imply that neonatal CD11c+ DC are more easily activated than adult CD11c+ DC, and /or be a consequence of the predominance of different DC subsets in neonatal and adult mice.en
dc.language.isoenen
dc.publisherScandinavian journal of immunologyen
dc.relation.urlhttp://dx.doi.org/10.1111/j.1365-3083.2008.02165.xen
dc.subject.meshAdjuvants, Immunologicen
dc.subject.meshAnimalsen
dc.subject.meshAnimals, Newbornen
dc.subject.meshAntigens, CD11cen
dc.subject.meshAntigens, CD40en
dc.subject.meshAntigens, CD86en
dc.subject.meshBacterial Toxinsen
dc.subject.meshCpG Islandsen
dc.subject.meshDendritic Cellsen
dc.subject.meshEnterotoxinsen
dc.subject.meshEnzyme-Linked Immunosorbent Assayen
dc.subject.meshEscherichia coli Proteinsen
dc.subject.meshFlow Cytometryen
dc.subject.meshHistocompatibility Antigens Class IIen
dc.subject.meshInterleukinsen
dc.subject.meshLymphocyte Activationen
dc.subject.meshMiceen
dc.subject.meshOligodeoxyribonucleotidesen
dc.subject.meshPneumococcal Vaccinesen
dc.subject.meshStatistics, Nonparametricen
dc.titleAdjuvants LT-K63 and CpG enhance the activation of dendritic cells in neonatal miceen
dc.typeArticleen
dc.contributor.departmentDepartment of Immunology, Landspitali, Iceland.en
dc.identifier.journalScandinavian journal of immunologyen

Related articles on PubMed

All Items in Hirsla are protected by copyright, with all rights reserved, unless otherwise indicated.