Enzyme immunoassays for measuring complement-dependent prevention of immune precipitation (PIP) and solubilization of preformed antigen-antibody complexes (SOL).

2.50
Hdl Handle:
http://hdl.handle.net/2336/46998
Title:
Enzyme immunoassays for measuring complement-dependent prevention of immune precipitation (PIP) and solubilization of preformed antigen-antibody complexes (SOL).
Authors:
Arason, G J; D'Ambrogio, M S; Vikingsdottir, T; Sigfusson, A; Valdimarsson, H
Citation:
J. Immunol. Methods.1999, 223(1):37-46
Issue Date:
1-Feb-1999
Abstract:
We have developed simple and sensitive enzyme-based methods for evaluating the ability of serum complement to prevent immune complex precipitation (PIP) or to solubilize preformed immune complexes (SOL). Alkaline phosphatase, serving both as antigen and label, is added to goat IgG anti-alkaline phosphatase antibodies, with serum present throughout the assay (PIP), or added after immune complex formation (SOL). After incubation at 37 degrees C for 1 h followed by centrifugation, the enzyme activity of the supernatant, reflecting the amount of immune complexes in solution, is measured by colorimetry. Results are expressed with reference to a standard serum pool assigned 100 arbitrary units (AU). Intra- and inter-assay variabilities are within 10%. The normal ranges were 67-133 AU for PIP and 72-129 AU for SOL. These methods have been standardized for clinical use in relation to impaired complement function and immune complex disease, and adapted for measuring complement mediated binding of immune complexes to erythrocytes. They are sensitive, easy to perform and do not require expensive facilities. By measuring the interaction of complement with immune complexes, these methods may highlight aspects of the classical and the alternative pathway that are different from those detected using haemolysis as an endpoint.
Description:
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Additional Links:
http://www.sciencedirect.com/science/article/B6T2Y-3VM79Y0-4/2/e3d0932ce1f75d1e030d6b6db5c991e0

Full metadata record

DC FieldValue Language
dc.contributor.authorArason, G J-
dc.contributor.authorD'Ambrogio, M S-
dc.contributor.authorVikingsdottir, T-
dc.contributor.authorSigfusson, A-
dc.contributor.authorValdimarsson, H-
dc.date.accessioned2009-01-02T10:58:11Z-
dc.date.available2009-01-02T10:58:11Z-
dc.date.issued1999-02-01-
dc.date.submitted2009-01-02-
dc.identifier.citationJ. Immunol. Methods.1999, 223(1):37-46en
dc.identifier.issn0022-1759-
dc.identifier.pmid10037233-
dc.identifier.doi10.1016/S0022-1759(98)00199-9-
dc.identifier.urihttp://hdl.handle.net/2336/46998-
dc.descriptionTo access publisher full text version of this article. Please click on the hyperlink in Additional Links fielden
dc.description.abstractWe have developed simple and sensitive enzyme-based methods for evaluating the ability of serum complement to prevent immune complex precipitation (PIP) or to solubilize preformed immune complexes (SOL). Alkaline phosphatase, serving both as antigen and label, is added to goat IgG anti-alkaline phosphatase antibodies, with serum present throughout the assay (PIP), or added after immune complex formation (SOL). After incubation at 37 degrees C for 1 h followed by centrifugation, the enzyme activity of the supernatant, reflecting the amount of immune complexes in solution, is measured by colorimetry. Results are expressed with reference to a standard serum pool assigned 100 arbitrary units (AU). Intra- and inter-assay variabilities are within 10%. The normal ranges were 67-133 AU for PIP and 72-129 AU for SOL. These methods have been standardized for clinical use in relation to impaired complement function and immune complex disease, and adapted for measuring complement mediated binding of immune complexes to erythrocytes. They are sensitive, easy to perform and do not require expensive facilities. By measuring the interaction of complement with immune complexes, these methods may highlight aspects of the classical and the alternative pathway that are different from those detected using haemolysis as an endpoint.en
dc.language.isoenen
dc.publisherNorth-Holland Pub. Coen
dc.relation.urlhttp://www.sciencedirect.com/science/article/B6T2Y-3VM79Y0-4/2/e3d0932ce1f75d1e030d6b6db5c991e0en
dc.subject.meshAlkaline Phosphataseen
dc.subject.meshAntigen-Antibody Complexen
dc.subject.meshCentrifugationen
dc.subject.meshComplement C2en
dc.subject.meshComplement System Proteinsen
dc.subject.meshDose-Response Relationship, Immunologicen
dc.subject.meshHumansen
dc.subject.meshImmune Complex Diseasesen
dc.subject.meshImmunoenzyme Techniquesen
dc.subject.meshPrecipitin Testsen
dc.subject.meshReference Valuesen
dc.subject.meshReproducibility of Resultsen
dc.subject.meshSolubilityen
dc.subject.meshTime Factorsen
dc.titleEnzyme immunoassays for measuring complement-dependent prevention of immune precipitation (PIP) and solubilization of preformed antigen-antibody complexes (SOL).en
dc.typeArticleen
dc.contributor.departmentDepartment of Immunology, National University Hospital, Landspítalinn, Reykjavík, Icelanden
dc.identifier.journalJournal of immunological methodsen

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