Plasma homocysteine and markers for oxidative stress and inflammation in patients with coronary artery disease--a prospective randomized study of vitamin supplementation.

2.50
Hdl Handle:
http://hdl.handle.net/2336/54173
Title:
Plasma homocysteine and markers for oxidative stress and inflammation in patients with coronary artery disease--a prospective randomized study of vitamin supplementation.
Authors:
Jonasson, Torfi; Ohlin, Ann-Kristin; Gottsäter, Anders; Hultberg, Björn; Ohlin, Hans
Citation:
Clin. Chem. Lab. Med. 2005, 43(6):628-34
Issue Date:
1-Jun-2005
Abstract:
BACKGROUND: Elevated plasma levels of total homocysteine (tHcy) are associated with an increased risk of developing occlusive vascular diseases. To better illustrate the relationship between plasma tHcy concentration, oxidative stress, and inflammation in patients with coronary artery disease (CAD), we measured plasma 8-isoprostane-prostaglandin F 2 (Iso-P), plasma malondialdehyde (MDA), and several markers of inflammation. We also aimed to demonstrate the effects of vitamin supplementation on these markers. METHODS: A total of 93 patients with ischemic heart disease were investigated. Of these, 34 had plasma tHcy < or =8 micromol/L, while 59 had plasma tHcy > or = 15.0 micromol/L. The 59 patients were randomized to open therapy with folic acid, 5 mg, pyridoxine, 40 mg, and cyancobalamin, 1 mg once daily for 3 months (n = 29) or to no vitamin treatment (n = 30). Blood samples were obtained from both groups before randomization and 3 months later. A sample was also obtained from the remaining 34 patients. RESULTS: Plasma Iso-P, serum amyloid A (S-AA), and plasma intercellular adhesion molecule-1 (ICAM-1) concentrations were higher in patients with high plasma tHcy levels than in patients with low to normal tHcy levels. Plasma levels of P-, L-, E-selectins, MDA, C-reactive protein (CRP), and orosomucoid did not differ between the groups. Vitamin therapy reduced plasma tHcy from 17.4 (15.3/20.1) to 9.2 (8.3/10.3) micromol/L (25th and 75th percentiles in parentheses) (p<0.0001). Plasma levels of Iso-P remained unchanged and, of all inflammatory markers, only the S-AA concentrations were slightly reduced by the vitamin treatment, from 5.3 (2.2/7.0) ng/L at baseline to 4.6 (2.1/6.9) ng/L (p<0.05) after 3 months of vitamin supplementation. CONCLUSION: Patients with CAD and high plasma tHcy levels had elevated plasma levels of Iso-P. The increase remained unaffected by plasma tHcy-lowering therapy, suggesting that homocysteine per se does not cause increased lipid peroxidation. Levels of plasma ICAM-1 and S-AA were increased in patients with high plasma tHcy, suggesting an association between homocysteinemia and low-grade inflammation.
Description:
To access publisher full text version of this article. Please click on the hyperlink in Additional Links field
Additional Links:
http://dx.doi.org/10.1515/CCLM.2005.108

Full metadata record

DC FieldValue Language
dc.contributor.authorJonasson, Torfi-
dc.contributor.authorOhlin, Ann-Kristin-
dc.contributor.authorGottsäter, Anders-
dc.contributor.authorHultberg, Björn-
dc.contributor.authorOhlin, Hans-
dc.date.accessioned2009-03-11T10:55:58Z-
dc.date.available2009-03-11T10:55:58Z-
dc.date.issued2005-06-01-
dc.date.submitted2009-05-11-
dc.identifier.citationClin. Chem. Lab. Med. 2005, 43(6):628-34en
dc.identifier.issn1434-6621-
dc.identifier.pmid16006259-
dc.identifier.doi10.1515/CCLM.2005.108-
dc.identifier.urihttp://hdl.handle.net/2336/54173-
dc.descriptionTo access publisher full text version of this article. Please click on the hyperlink in Additional Links fielden
dc.description.abstractBACKGROUND: Elevated plasma levels of total homocysteine (tHcy) are associated with an increased risk of developing occlusive vascular diseases. To better illustrate the relationship between plasma tHcy concentration, oxidative stress, and inflammation in patients with coronary artery disease (CAD), we measured plasma 8-isoprostane-prostaglandin F 2 (Iso-P), plasma malondialdehyde (MDA), and several markers of inflammation. We also aimed to demonstrate the effects of vitamin supplementation on these markers. METHODS: A total of 93 patients with ischemic heart disease were investigated. Of these, 34 had plasma tHcy < or =8 micromol/L, while 59 had plasma tHcy > or = 15.0 micromol/L. The 59 patients were randomized to open therapy with folic acid, 5 mg, pyridoxine, 40 mg, and cyancobalamin, 1 mg once daily for 3 months (n = 29) or to no vitamin treatment (n = 30). Blood samples were obtained from both groups before randomization and 3 months later. A sample was also obtained from the remaining 34 patients. RESULTS: Plasma Iso-P, serum amyloid A (S-AA), and plasma intercellular adhesion molecule-1 (ICAM-1) concentrations were higher in patients with high plasma tHcy levels than in patients with low to normal tHcy levels. Plasma levels of P-, L-, E-selectins, MDA, C-reactive protein (CRP), and orosomucoid did not differ between the groups. Vitamin therapy reduced plasma tHcy from 17.4 (15.3/20.1) to 9.2 (8.3/10.3) micromol/L (25th and 75th percentiles in parentheses) (p<0.0001). Plasma levels of Iso-P remained unchanged and, of all inflammatory markers, only the S-AA concentrations were slightly reduced by the vitamin treatment, from 5.3 (2.2/7.0) ng/L at baseline to 4.6 (2.1/6.9) ng/L (p<0.05) after 3 months of vitamin supplementation. CONCLUSION: Patients with CAD and high plasma tHcy levels had elevated plasma levels of Iso-P. The increase remained unaffected by plasma tHcy-lowering therapy, suggesting that homocysteine per se does not cause increased lipid peroxidation. Levels of plasma ICAM-1 and S-AA were increased in patients with high plasma tHcy, suggesting an association between homocysteinemia and low-grade inflammation.en
dc.language.isoenen
dc.publisherWalter De Gruyteren
dc.relation.urlhttp://dx.doi.org/10.1515/CCLM.2005.108en
dc.subject.meshAgeden
dc.subject.meshBiological Markersen
dc.subject.meshCoronary Artery Diseaseen
dc.subject.meshFemaleen
dc.subject.meshFolic Aciden
dc.subject.meshHomocysteineen
dc.subject.meshHumansen
dc.subject.meshHyperhomocysteinemiaen
dc.subject.meshInflammationen
dc.subject.meshLipid Peroxidationen
dc.subject.meshMaleen
dc.subject.meshMiddle Ageden
dc.subject.meshOxidative Stressen
dc.subject.meshPyridoxineen
dc.subject.meshVitamin B 12en
dc.subject.meshVitaminsen
dc.titlePlasma homocysteine and markers for oxidative stress and inflammation in patients with coronary artery disease--a prospective randomized study of vitamin supplementation.en
dc.typeArticleen
dc.contributor.departmentDepartment of Cardiology, University Hospital Lund, Lund, Sweden. torfifja@landspitali.isen
dc.identifier.journalClinical chemistry and laboratory medicine : CCLM / FESCCen

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