2.50
Hdl Handle:
http://hdl.handle.net/2336/58093
Title:
GABA agonists fail to protect the retina from ischemia-reperfusion injury
Authors:
Traustason, Sindri; Eysteinsson, Thor; Agnarsson, Bjarni A; Stefansson, Einar
Citation:
Exp. Eye Res. 2009, 88(3):361-6
Issue Date:
1-Mar-2009
Abstract:
The purpose of this study was to test the hypothesis that ischemia/reperfusion injury in the rat retina may be ameliorated by reducing retinal metabolism with either hypothermia or inhibitory GABA agonists. The intraocular pressure of each right eye in rats was raised to 130 mm Hg for 60 min with the left eye serving as normal control. The rats were divided into four groups in terms of drug and hypothermia treatment: (1) Untreated ischemia, (2) Hypothermia, (3) Baclofen/midazolam and (4) Baclofen/muscimol. Electroretinogram was recorded before ischemia and again after 10-day reperfusion. Histological analysis with H&E staining and cell counts was performed. Untreated ischemia/reperfusion resulted in severely reduced ERG responses. The ERG b-wave was reduced from 423+/-144 microV to 130+/-91 microV (mean+/-SD, n=5). With hypothermia the ERG b-wave was reduced from 499+/-80 microV to 237+/-111 microV (n=4). With combinations of baclofen and midazolam the ERG b-wave was reduced from 432+/-96 microV to 104+/-67 microV (n=7). In baclofen/muscimol treated eyes the ERG b-wave went from 426+/-101 microV to 148+/-118 microV (n=6). The histological tissue damage was severe in untreated ischemia and the baclofen/midazolam and baclofen/muscimol groups, but less severe in the hypothermia group. The GABA agonists do not provide any protection in our ischemia/reperfusion model. Our results are consistent with earlier reports that hypothermia may be helpful in ischemic conditions in the retina.
Description:
To access publisher full text version of this article. Please click on the hyperlink in Additional Links field
Additional Links:
http://www.sciencedirect.com/science/article/B6WFD-4V42JBN-2/2/b850bc0d895e5e351dbe894e1282a4a0

Full metadata record

DC FieldValue Language
dc.contributor.authorTraustason, Sindri-
dc.contributor.authorEysteinsson, Thor-
dc.contributor.authorAgnarsson, Bjarni A-
dc.contributor.authorStefansson, Einar-
dc.date.accessioned2009-03-31T09:54:41Z-
dc.date.available2009-03-31T09:54:41Z-
dc.date.issued2009-03-01-
dc.date.submitted2009-03-31-
dc.identifier.citationExp. Eye Res. 2009, 88(3):361-6en
dc.identifier.issn1096-0007-
dc.identifier.pmid19101544-
dc.identifier.doi10.1016/j.exer.2008.07.019-
dc.identifier.urihttp://hdl.handle.net/2336/58093-
dc.descriptionTo access publisher full text version of this article. Please click on the hyperlink in Additional Links fielden
dc.description.abstractThe purpose of this study was to test the hypothesis that ischemia/reperfusion injury in the rat retina may be ameliorated by reducing retinal metabolism with either hypothermia or inhibitory GABA agonists. The intraocular pressure of each right eye in rats was raised to 130 mm Hg for 60 min with the left eye serving as normal control. The rats were divided into four groups in terms of drug and hypothermia treatment: (1) Untreated ischemia, (2) Hypothermia, (3) Baclofen/midazolam and (4) Baclofen/muscimol. Electroretinogram was recorded before ischemia and again after 10-day reperfusion. Histological analysis with H&E staining and cell counts was performed. Untreated ischemia/reperfusion resulted in severely reduced ERG responses. The ERG b-wave was reduced from 423+/-144 microV to 130+/-91 microV (mean+/-SD, n=5). With hypothermia the ERG b-wave was reduced from 499+/-80 microV to 237+/-111 microV (n=4). With combinations of baclofen and midazolam the ERG b-wave was reduced from 432+/-96 microV to 104+/-67 microV (n=7). In baclofen/muscimol treated eyes the ERG b-wave went from 426+/-101 microV to 148+/-118 microV (n=6). The histological tissue damage was severe in untreated ischemia and the baclofen/midazolam and baclofen/muscimol groups, but less severe in the hypothermia group. The GABA agonists do not provide any protection in our ischemia/reperfusion model. Our results are consistent with earlier reports that hypothermia may be helpful in ischemic conditions in the retina.en
dc.language.isoenen
dc.publisherAcademic Pressen
dc.relation.urlhttp://www.sciencedirect.com/science/article/B6WFD-4V42JBN-2/2/b850bc0d895e5e351dbe894e1282a4a0en
dc.subject.meshRetinaen
dc.subject.meshIschemiaen
dc.subject.meshReperfusionen
dc.subject.meshPubMed - in processen
dc.titleGABA agonists fail to protect the retina from ischemia-reperfusion injuryen
dc.typeArticleen
dc.contributor.departmentDepartment of Ophthalmology and Physiology, National University Hospital, University of Iceland, Reykjavík, Iceland.en
dc.identifier.journalExperimental eye researchen

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