Genetic approaches to assessing evidence for a T helper type 1 cytokine defect in adult asthma

2.50
Hdl Handle:
http://hdl.handle.net/2336/58913
Title:
Genetic approaches to assessing evidence for a T helper type 1 cytokine defect in adult asthma
Authors:
Birkisson, Illugi F; Halapi, Eva; Bjornsdottir, Unnur S; Shkolny, Dana L; Adalsteinsdottir, Elva; Arnason, Thor; Gislason, David; Gislason, Thorarinn; Gulcher, Jeffrey; Stefansson, Kari; Hakonarson, Hakon
Citation:
Am. J. Respir. Crit. Care Med. 2004, 169(9):1007-13
Issue Date:
1-May-2004
Abstract:
Recent evidence suggests that deficiency in the Th1 cytokine pathway may underlie the susceptibility to allergic asthma. This study examined whether (1) single-nucleotide polymorphisms exist in the promoter region of the two interleukin (IL)-12 subunit genes in patients with asthma; (2) messenger RNA and protein expressions of signal transducers and activators of transcription, IL-12, IFN-gamma, and their receptors are altered in asthma; and (3) linkage to genes in the Th1 pathway is present in families with asthma in Iceland. The promoter regions of the IL-12 subunit genes were sequenced in 94 patients with asthma and 94 control subjects without asthma. Linkage was examined in 169 families that included over 570 patients with asthma and 950 of their unaffected relatives. The results demonstrate no evidence of linkage to microsatellite markers in close association with genes within the Th1 pathway, and no polymorphism was detected in the promoter regions of the two IL-12 subunit genes in the cohort with asthma patients. Moreover, we found no differences in the messenger RNA or protein expression signals of genes in the IL-12 pathway between the patients and control subjects. We conclude that decrease in Th1 type cytokine response is unlikely to present a primary event in asthma.
Description:
To access publisher full text version of this article. Please click on the hyperlink in Additional Links field
Additional Links:
http://ajrccm.atsjournals.org/cgi/content/abstract/169/9/1007

Full metadata record

DC FieldValue Language
dc.contributor.authorBirkisson, Illugi F-
dc.contributor.authorHalapi, Eva-
dc.contributor.authorBjornsdottir, Unnur S-
dc.contributor.authorShkolny, Dana L-
dc.contributor.authorAdalsteinsdottir, Elva-
dc.contributor.authorArnason, Thor-
dc.contributor.authorGislason, David-
dc.contributor.authorGislason, Thorarinn-
dc.contributor.authorGulcher, Jeffrey-
dc.contributor.authorStefansson, Kari-
dc.contributor.authorHakonarson, Hakon-
dc.date.accessioned2009-04-02T13:38:30Z-
dc.date.available2009-04-02T13:38:30Z-
dc.date.issued2004-05-01-
dc.date.submitted2009-04-02-
dc.identifier.citationAm. J. Respir. Crit. Care Med. 2004, 169(9):1007-13en
dc.identifier.issn1073-449X-
dc.identifier.pmid14962816-
dc.identifier.doi10.1164/rccm.200302-228OC-
dc.identifier.urihttp://hdl.handle.net/2336/58913-
dc.descriptionTo access publisher full text version of this article. Please click on the hyperlink in Additional Links fielden
dc.description.abstractRecent evidence suggests that deficiency in the Th1 cytokine pathway may underlie the susceptibility to allergic asthma. This study examined whether (1) single-nucleotide polymorphisms exist in the promoter region of the two interleukin (IL)-12 subunit genes in patients with asthma; (2) messenger RNA and protein expressions of signal transducers and activators of transcription, IL-12, IFN-gamma, and their receptors are altered in asthma; and (3) linkage to genes in the Th1 pathway is present in families with asthma in Iceland. The promoter regions of the IL-12 subunit genes were sequenced in 94 patients with asthma and 94 control subjects without asthma. Linkage was examined in 169 families that included over 570 patients with asthma and 950 of their unaffected relatives. The results demonstrate no evidence of linkage to microsatellite markers in close association with genes within the Th1 pathway, and no polymorphism was detected in the promoter regions of the two IL-12 subunit genes in the cohort with asthma patients. Moreover, we found no differences in the messenger RNA or protein expression signals of genes in the IL-12 pathway between the patients and control subjects. We conclude that decrease in Th1 type cytokine response is unlikely to present a primary event in asthma.en
dc.language.isoenen
dc.publisherAmerican Thoracic Societyen
dc.relation.urlhttp://ajrccm.atsjournals.org/cgi/content/abstract/169/9/1007en
dc.subject.meshAdolescenten
dc.subject.meshAdulten
dc.subject.meshAsthmaen
dc.subject.meshCase-Control Studiesen
dc.subject.meshChilden
dc.subject.meshEnzyme-Linked Immunosorbent Assayen
dc.subject.meshFemaleen
dc.subject.meshFlow Cytometryen
dc.subject.meshGenotypeen
dc.subject.meshHumansen
dc.subject.meshHypersensitivity, Immediateen
dc.subject.meshInterferon-gammaen
dc.subject.meshInterleukin-12en
dc.subject.meshLinkage (Genetics)en
dc.subject.meshMaleen
dc.subject.meshMicrosatellite Repeatsen
dc.subject.meshMiddle Ageden
dc.subject.meshPhenotypeen
dc.subject.meshPolymerase Chain Reactionen
dc.subject.meshPolymorphism, Single Nucleotideen
dc.subject.meshPromoter Regions, Geneticen
dc.subject.meshRNA, Messengeren
dc.subject.meshSignal Transductionen
dc.subject.meshTh1 Cellsen
dc.subject.meshTranscription, Geneticen
dc.titleGenetic approaches to assessing evidence for a T helper type 1 cytokine defect in adult asthmaen
dc.typeArticleen
dc.contributor.departmentThe Medical University of Iceland, Reykjavik, Iceland.en
dc.identifier.journalAmerican journal of respiratory and critical care medicineen

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