Novel effects of azithromycin on tight junction proteins in human airway epithelia

2.50
Hdl Handle:
http://hdl.handle.net/2336/6144
Title:
Novel effects of azithromycin on tight junction proteins in human airway epithelia
Authors:
Asgrimsson, Valthor; Gudjonsson, Thorarinn; Gudmundsson, Gudmundur Hrafn; Baldursson, Olafur
Issue Date:
5-Jan-2006
Abstract:
The macrolide antibiotic azithromycin improves lung function and prognosis among patients with cystic fibrosis or diffuse panbronchiolitis, independently of bacterial eradication. Anti-inflammatory effects have been implicated, but data from in vivo studies are scarce, and the link between abnormal electrolyte content in airway surface liquid and bronchial infections remains uncertain. In the present study, we treated human airway epithelia on filter supports with azithromycin and monitored transepithelial electrical resistance. We found that azithromycin increased transepithelial electrical resistance of airway epithelia in a dose-dependent manner. Immunocytochemistry and Western blotting showed that addition of azithromycin changed the locations of proteins in cell cultures and induced processing of the tight junction proteins claudin-1 and claudin-4, occludin, and junctional adhesion molecule-A. These effects were reversible, and no effect was seen when cells were treated with penicillin or erythromycin. The data indicate that azithromycin increases the transepithelial electrical resistance of human airway epithelia by changing the processing of tight junction proteins. The results are novel and may help explain the beneficial effects of azithromycin in patients with cystic fibrosis, diffuse panbronchiolitis, and community-acquired pneumonia.
Additional Links:
http://aac.asm.org/cgi/content/full/50/5/1805?view=long&pmid=16641453; http://www.pubmedcentral.nih.gov/picrender.fcgi?artid=1472195&blobtype=pdf

Full metadata record

DC FieldValue Language
dc.contributor.authorAsgrimsson, Valthor-
dc.contributor.authorGudjonsson, Thorarinn-
dc.contributor.authorGudmundsson, Gudmundur Hrafn-
dc.contributor.authorBaldursson, Olafur-
dc.date.accessioned2006-11-17T15:17:29Z-
dc.date.available2006-11-17T15:17:29Z-
dc.date.issued2006-01-05-
dc.date.submitted2006-17-11-
dc.identifier.pmid16641453-
dc.identifier.doi10.1128/AAC.50.5.1805-1812.2006-
dc.identifier.otherPAD12-
dc.identifier.urihttp://hdl.handle.net/2336/6144-
dc.description.abstractThe macrolide antibiotic azithromycin improves lung function and prognosis among patients with cystic fibrosis or diffuse panbronchiolitis, independently of bacterial eradication. Anti-inflammatory effects have been implicated, but data from in vivo studies are scarce, and the link between abnormal electrolyte content in airway surface liquid and bronchial infections remains uncertain. In the present study, we treated human airway epithelia on filter supports with azithromycin and monitored transepithelial electrical resistance. We found that azithromycin increased transepithelial electrical resistance of airway epithelia in a dose-dependent manner. Immunocytochemistry and Western blotting showed that addition of azithromycin changed the locations of proteins in cell cultures and induced processing of the tight junction proteins claudin-1 and claudin-4, occludin, and junctional adhesion molecule-A. These effects were reversible, and no effect was seen when cells were treated with penicillin or erythromycin. The data indicate that azithromycin increases the transepithelial electrical resistance of human airway epithelia by changing the processing of tight junction proteins. The results are novel and may help explain the beneficial effects of azithromycin in patients with cystic fibrosis, diffuse panbronchiolitis, and community-acquired pneumonia.en
dc.format.extent86519 bytes-
dc.format.extent1035264 bytes-
dc.format.mimetypeapplication/pdf-
dc.format.mimetypeapplication/vnd.ms-powerpoint-
dc.language.isoenen
dc.relation.urlhttp://aac.asm.org/cgi/content/full/50/5/1805?view=long&pmid=16641453en
dc.relation.urlhttp://www.pubmedcentral.nih.gov/picrender.fcgi?artid=1472195&blobtype=pdfen
dc.subject.meshAnti-Bacterial Agentsen
dc.subject.meshAzithromycinen
dc.subject.meshBlotting, Westernen
dc.subject.meshBronchien
dc.subject.meshCadherinsen
dc.subject.meshCell Adhesion Moleculesen
dc.subject.meshCell Line, Transformeden
dc.subject.meshCell Transformation, Viralen
dc.subject.meshComparative Studyen
dc.subject.meshDose-Response Relationship, Drugen
dc.subject.meshElectric Impedanceen
dc.subject.meshEpithelial Cellsen
dc.subject.meshImmunohistochemistryen
dc.subject.meshMembrane Proteinsen
dc.subject.meshTight Junctionsen
dc.subject.meshTime Factorsen
dc.titleNovel effects of azithromycin on tight junction proteins in human airway epitheliaen
dc.typeManuscripten
dc.format.digYES-

Related articles on PubMed

All Items in Hirsla are protected by copyright, with all rights reserved, unless otherwise indicated.