Clinical evaluation of a rapid polymerase chain reaction (PCR) assay : for the detection of chlamydia trachomatis in specimens from high risk patients

2.50
Hdl Handle:
http://hdl.handle.net/2336/68314
Title:
Clinical evaluation of a rapid polymerase chain reaction (PCR) assay : for the detection of chlamydia trachomatis in specimens from high risk patients
Authors:
Ólafur Steingrímsson; Jón Hjaltalín Ólafsson; Sigfús M. Karlsson; Rannveig Pálsdóttir
Citation:
Læknablaðið 1995, 81(7):531-4
Issue Date:
1-Jul-1995
Abstract:
A Rapid Polymerase Chain Reaction Assay (Ampli-cor®-PCR) was evaluated for the detection of Chlamydia trachomatis in specimens from 179 high risk patients. The results were compared to McCoy cell culture and specimens were retested with Amplicor® and primers for the Major Outer Membrane Protein (MOMP) gene when discrepancy occurred. Of 88 females enrolled in the study, 30 were infected (34%). Sensitivity, selectivity, predictive value of a positive (PVP) and a negative (PVN) on endocervical specimens were 97%, 96.5%, 96.5% and 98% respectively. Of 91 male urine specimens, 33 (36%) came from infected patients. The sensitivity and specificity of the Amplicor® assay was 94% and 74% respectively for male urine specimens and the PVP and PVN were 72% and 96% respectively. The sensitivity was low on the original run on urethral specimens but the majority of false negative specimens became positive when retested. Amplicor® performed on urine samples was the most sensitive test for detecting Chlamydial infections in males.
Description:
Neðst á síðunni er hægt að nálgast greinina í heild sinni með því að smella á hlekkinn View/Open
Additional Links:
http://www.laeknabladid.is

Full metadata record

DC FieldValue Language
dc.contributor.authorÓlafur Steingrímsson-
dc.contributor.authorJón Hjaltalín Ólafsson-
dc.contributor.authorSigfús M. Karlsson-
dc.contributor.authorRannveig Pálsdóttir-
dc.date.accessioned2009-05-15T11:39:03Z-
dc.date.available2009-05-15T11:39:03Z-
dc.date.issued1995-07-01-
dc.date.submitted2009-05-15-
dc.identifier.citationLæknablaðið 1995, 81(7):531-4en
dc.identifier.issn0023-7213-
dc.identifier.urihttp://hdl.handle.net/2336/68314-
dc.descriptionNeðst á síðunni er hægt að nálgast greinina í heild sinni með því að smella á hlekkinn View/Openen
dc.description.abstractA Rapid Polymerase Chain Reaction Assay (Ampli-cor®-PCR) was evaluated for the detection of Chlamydia trachomatis in specimens from 179 high risk patients. The results were compared to McCoy cell culture and specimens were retested with Amplicor® and primers for the Major Outer Membrane Protein (MOMP) gene when discrepancy occurred. Of 88 females enrolled in the study, 30 were infected (34%). Sensitivity, selectivity, predictive value of a positive (PVP) and a negative (PVN) on endocervical specimens were 97%, 96.5%, 96.5% and 98% respectively. Of 91 male urine specimens, 33 (36%) came from infected patients. The sensitivity and specificity of the Amplicor® assay was 94% and 74% respectively for male urine specimens and the PVP and PVN were 72% and 96% respectively. The sensitivity was low on the original run on urethral specimens but the majority of false negative specimens became positive when retested. Amplicor® performed on urine samples was the most sensitive test for detecting Chlamydial infections in males.en
dc.language.isoenen
dc.publisherLæknafélag Íslands, Læknafélag Reykjavíkuren
dc.relation.urlhttp://www.laeknabladid.isen
dc.subjectKynsjúkdómaren
dc.subjectKlamýdíaen
dc.subject.meshSexually Transmitted Diseasesen
dc.subject.meshPolymerase Chain Reactionen
dc.subject.meshChlamydia trachomatisen
dc.titleClinical evaluation of a rapid polymerase chain reaction (PCR) assay : for the detection of chlamydia trachomatis in specimens from high risk patientsen
dc.typeArticleen
dc.identifier.journalLæknablaðiðen
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