2.50
Hdl Handle:
http://hdl.handle.net/2336/73353
Title:
Immune response against equine gammaherpesvirus in Icelandic horses
Authors:
Svansson, V; Roelse, M; Olafsdottir, G; Thorsteinsdottir, L; Torfason, E G; Torsteinsdottir, S
Citation:
Vet. Microbiol. 2009, 137(3-4):363-8
Issue Date:
12-Jun-2009
Abstract:
Horses are hosts to two types of gammaherpesviruses, equine herpes virus (EHV) 2 and 5. While EHV-2 is ubiquitous in adult horses, EHV-5 has been less frequently described. Due to strong serological cross-reactivity, EHV-2 and -5 cannot be discriminated in broad spectrum antibody tests and are thus commonly referred to as gamma-EHV. Total IgG and IgG subclass response against gamma-EHV were determined in serum from 41 healthy Icelandic horses, thereof 20 adults, 10 foals aged 10 months, and 11 foals aged 1-4 months. Additionally, in 10 of the adult horses, interferon (IFN)-gamma and interleukin (IL)-4 expression were measured by real-time PCR in white blood cells upon in vitro stimulation with EHV-2. With the exception of one orphan foal, all tested individuals were seropositive for gamma-EHV. All but one adult had high titer of EHV-specific IgG4/7 (IgGb) in combination with much lower titer of IgG1 (IgGa) and IgG3/5 (IgG(T)), indicating a stabilized response. IgG titer and subclasses in the foals showed considerably more variation, possibly dependant on maternal antibodies and/or recent infection. In all the 10 horses tested for cytokine expression, IFN-gamma production exceeds production of IL-4. These results indicate that equine gammaherpesvirus infection is characterized by an induction of IgG1, IgG4/7 and IgG3/5 with prevailing IgG4/7 and cytokine profile dominated by IFN-gamma. To our knowledge, this is the first report on the cytokine and IgG subclass response against gamma-EHV in horses.
Description:
To access publisher full text version of this article. Please click on the hyperlink in Additional Links field
Additional Links:
http://dx.doi.org/10.1016/j.vetmic.2009.01.020

Full metadata record

DC FieldValue Language
dc.contributor.authorSvansson, V-
dc.contributor.authorRoelse, M-
dc.contributor.authorOlafsdottir, G-
dc.contributor.authorThorsteinsdottir, L-
dc.contributor.authorTorfason, E G-
dc.contributor.authorTorsteinsdottir, S-
dc.date.accessioned2009-07-10T14:23:57Z-
dc.date.available2009-07-10T14:23:57Z-
dc.date.issued2009-06-12-
dc.date.submitted2009-07-10-
dc.identifier.citationVet. Microbiol. 2009, 137(3-4):363-8en
dc.identifier.issn0378-1135-
dc.identifier.pmid19217222-
dc.identifier.doi10.1016/j.vetmic.2009.01.020-
dc.identifier.urihttp://hdl.handle.net/2336/73353-
dc.descriptionTo access publisher full text version of this article. Please click on the hyperlink in Additional Links fielden
dc.description.abstractHorses are hosts to two types of gammaherpesviruses, equine herpes virus (EHV) 2 and 5. While EHV-2 is ubiquitous in adult horses, EHV-5 has been less frequently described. Due to strong serological cross-reactivity, EHV-2 and -5 cannot be discriminated in broad spectrum antibody tests and are thus commonly referred to as gamma-EHV. Total IgG and IgG subclass response against gamma-EHV were determined in serum from 41 healthy Icelandic horses, thereof 20 adults, 10 foals aged 10 months, and 11 foals aged 1-4 months. Additionally, in 10 of the adult horses, interferon (IFN)-gamma and interleukin (IL)-4 expression were measured by real-time PCR in white blood cells upon in vitro stimulation with EHV-2. With the exception of one orphan foal, all tested individuals were seropositive for gamma-EHV. All but one adult had high titer of EHV-specific IgG4/7 (IgGb) in combination with much lower titer of IgG1 (IgGa) and IgG3/5 (IgG(T)), indicating a stabilized response. IgG titer and subclasses in the foals showed considerably more variation, possibly dependant on maternal antibodies and/or recent infection. In all the 10 horses tested for cytokine expression, IFN-gamma production exceeds production of IL-4. These results indicate that equine gammaherpesvirus infection is characterized by an induction of IgG1, IgG4/7 and IgG3/5 with prevailing IgG4/7 and cytokine profile dominated by IFN-gamma. To our knowledge, this is the first report on the cytokine and IgG subclass response against gamma-EHV in horses.en
dc.language.isoenen
dc.publisherElsevier Scientific Pub. Co.en
dc.relation.urlhttp://dx.doi.org/10.1016/j.vetmic.2009.01.020en
dc.subject.meshHorsesen
dc.subject.meshHorse Diseasesen
dc.titleImmune response against equine gammaherpesvirus in Icelandic horsesen
dc.typeArticleen
dc.contributor.departmentInstitute for Experimental Pathology, University of Iceland, Keldur v/Vesturlandsveg, IS-112 Reykjavík, Iceland.en
dc.identifier.journalVeterinary microbiologyen

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