The complement regulatory proteins CD46 and CD59, but not CD55, are highly expressed by glandular epithelium of human breast and colorectal tumour tissues

2.50
Hdl Handle:
http://hdl.handle.net/2336/93638
Title:
The complement regulatory proteins CD46 and CD59, but not CD55, are highly expressed by glandular epithelium of human breast and colorectal tumour tissues
Authors:
Thorsteinsson, L; O'Dowd, G M; Harrington, P M; Johnson, P M
Citation:
APMIS 1998, 106(9):869-78
Issue Date:
1-Sep-1998
Abstract:
Three of the proteins protecting cells from autologous lysis by complement are: membrane cofactor protein (MCP; CD46), an inhibitor of the membrane attack complex formation (CD59), and decay accelerating factor (DAF; CD55). We have investigated the expression of these proteins in breast and colorectal carcinoma by immunohistochemistry and immunoblotting of breast tissue for CD46. CD46 was consistently and strongly expressed in the epithelial compartment in 26/28 ductal carcinomas of the breast, 9/9 fibroadenomas, and 9/11 cases of control non-neoplastic breast tissue. CD59 showed a similar degree of expression in the fibroadenomas (9/9), but was less strongly expressed in carcinomatous (22/28) and control (5/11) tissues. In marked contrast, no CD55 expression was detected in tissue from 15 ductal carcinomas. Immunoblotting of breast tissue for CD46 showed the same size of the molecule as for lymphocytes. It had however considerably stronger expression in tumour tissue than in non-neoplastic tissue. CD46 and CD59 were either lacking or only weakly expressed in the epithelial component of control colorectal mucosa: 2/15 and 5/15, respectively. In contrast, tissue samples from colorectal adenocarcinomas showed clear staining for both CD59 (10/18) and, more markedly, CD46 (15/18). There was no association between the pattern or intensity of CD46 and CD59 expression and tumour differentiation. As the complement regulatory proteins CD46 and CD59 are also strongly expressed by trophoblast at the feto-maternal tissue interface, these results support the concept that similar mechanisms are employed both by the genetically dissimilar fetus and certain tumours to evade immune attack by their host.
Description:
To access publisher full text version of this article. Please click on the hyperlink in Additional Links field
Additional Links:
http://dx.doi.org/10.1111/j.1699-0463.1998.tb00233.x

Full metadata record

DC FieldValue Language
dc.contributor.authorThorsteinsson, Len
dc.contributor.authorO'Dowd, G Men
dc.contributor.authorHarrington, P Men
dc.contributor.authorJohnson, P Men
dc.date.accessioned2010-03-04T11:48:47Z-
dc.date.available2010-03-04T11:48:47Z-
dc.date.issued1998-09-01-
dc.date.submitted2010-03-04-
dc.identifier.citationAPMIS 1998, 106(9):869-78en
dc.identifier.issn0903-4641-
dc.identifier.pmid9808413-
dc.identifier.doi10.1111/j.1699-0463.1998.tb00233.x-
dc.identifier.urihttp://hdl.handle.net/2336/93638-
dc.descriptionTo access publisher full text version of this article. Please click on the hyperlink in Additional Links fielden
dc.description.abstractThree of the proteins protecting cells from autologous lysis by complement are: membrane cofactor protein (MCP; CD46), an inhibitor of the membrane attack complex formation (CD59), and decay accelerating factor (DAF; CD55). We have investigated the expression of these proteins in breast and colorectal carcinoma by immunohistochemistry and immunoblotting of breast tissue for CD46. CD46 was consistently and strongly expressed in the epithelial compartment in 26/28 ductal carcinomas of the breast, 9/9 fibroadenomas, and 9/11 cases of control non-neoplastic breast tissue. CD59 showed a similar degree of expression in the fibroadenomas (9/9), but was less strongly expressed in carcinomatous (22/28) and control (5/11) tissues. In marked contrast, no CD55 expression was detected in tissue from 15 ductal carcinomas. Immunoblotting of breast tissue for CD46 showed the same size of the molecule as for lymphocytes. It had however considerably stronger expression in tumour tissue than in non-neoplastic tissue. CD46 and CD59 were either lacking or only weakly expressed in the epithelial component of control colorectal mucosa: 2/15 and 5/15, respectively. In contrast, tissue samples from colorectal adenocarcinomas showed clear staining for both CD59 (10/18) and, more markedly, CD46 (15/18). There was no association between the pattern or intensity of CD46 and CD59 expression and tumour differentiation. As the complement regulatory proteins CD46 and CD59 are also strongly expressed by trophoblast at the feto-maternal tissue interface, these results support the concept that similar mechanisms are employed both by the genetically dissimilar fetus and certain tumours to evade immune attack by their host.en
dc.language.isoenen
dc.publisherMunksgaarden
dc.relation.urlhttp://dx.doi.org/10.1111/j.1699-0463.1998.tb00233.xen
dc.subject.meshAdenocarcinomaen
dc.subject.meshAdulten
dc.subject.meshAgeden
dc.subject.meshAntigens, CDen
dc.subject.meshAntigens, CD46en
dc.subject.meshAntigens, CD55en
dc.subject.meshAntigens, CD59en
dc.subject.meshBlotting, Westernen
dc.subject.meshBreasten
dc.subject.meshBreast Neoplasmsen
dc.subject.meshCarcinoma, Ductal, Breasten
dc.subject.meshColorectal Neoplasmsen
dc.subject.meshComplement System Proteinsen
dc.subject.meshEnzyme-Linked Immunosorbent Assayen
dc.subject.meshEpithelial Cellsen
dc.subject.meshFemaleen
dc.subject.meshFibroadenomaen
dc.subject.meshHLA Antigensen
dc.subject.meshHumansen
dc.subject.meshImmunohistochemistryen
dc.subject.meshIntestinal Mucosaen
dc.subject.meshLymphocytesen
dc.subject.meshMembrane Glycoproteinsen
dc.subject.meshMiddle Ageden
dc.subject.meshStromal Cellsen
dc.titleThe complement regulatory proteins CD46 and CD59, but not CD55, are highly expressed by glandular epithelium of human breast and colorectal tumour tissuesen
dc.typeArticleen
dc.contributor.departmentCancer Tissue Bank Research Centre, and Department of Immunology, University of Liverpool, England.en
dc.identifier.journalAPMIS : acta pathologica, microbiologica, et immunologica Scandinavicaen
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