Show simple item record

dc.contributor.authorThormar, H
dc.contributor.authorBergsson, G
dc.contributor.authorGunnarsson, E
dc.contributor.authorGeorgsson, G
dc.contributor.authorWitvrouw, M
dc.contributor.authorSteingrimsson, Olafur
dc.contributor.authorDe Clercq, E
dc.contributor.authorKristmundsdottir, T
dc.date.accessioned2010-07-30T14:20:06Z
dc.date.available2010-07-30T14:20:06Z
dc.date.issued1999-06-01
dc.date.submitted2010-07-30
dc.identifier.citationSex Transm Infect. 1999, 75(3):181-5en
dc.identifier.issn1368-4973
dc.identifier.pmid10448397
dc.identifier.doi10.1136/sti.75.3.181
dc.identifier.urihttp://hdl.handle.net/2336/108727
dc.descriptionTo access publisher full text version of this article. Please click on the hyperlink in Additional Links fielden
dc.description.abstractOBJECTIVE: To investigate the in vitro microbicidal and cytocidal potency of monocaprin dissolved in pharmaceutical hydrogel formulations and to evaluate their potential use as vaginal microbicides against sexually transmitted pathogens such as herpes simplex virus type 2 (HSV-2), human immunodeficiency virus type 1 (HIV-1), Chlamydia trachomatis, and Neisseria gonorrhoeae. METHODS: Gel formulations were mixed with equal volumes of virus/bacteria suspensions in culture medium and incubated for 1 and 5 minutes. The reduction in virus/bacteria titre was used as a measure of microbicidal activity. Similarly, gels were mixed with human semen to study their effect on leucocytes. The toxicity of the gels was tested in rabbits by the standard vaginal irritation test. RESULTS: Gels containing 20 mM of monocaprin caused a greater than 100,000-fold inactivation of HSV-2 and Neisseria in 1 minute and of Chlamydia in 5 minutes. Similarly, the gels caused a greater than 10,000-fold inactivation of HIV-1 in semen in 1 minute. They caused more than a 10,000-fold reduction in the number of viable leucocytes in semen in 1 minute. No toxic effect on the vaginal mucosa of rabbits was observed after daily exposure for 10 days. CONCLUSIONS: Hydrogels containing monocaprin are potent inactivators of sexually transmitted viruses and bacteria in vitro. This simple lipid seems to be a feasible choice as a mucosal microbicide for prevention of sexually transmitted infections. It is a natural compound found in certain foodstuffs such as milk and is therefore unlikely to cause harmful side effects in the concentrations used.
dc.language.isoenen
dc.publisherBmj Publishing Groupen
dc.relation.urlhttp://dx.doi.org/10.1136/sti.75.3.181en
dc.subject.meshAnimalsen
dc.subject.meshAnti-Bacterial Agentsen
dc.subject.meshAnti-Infective Agentsen
dc.subject.meshCells, Cultureden
dc.subject.meshChlamydia trachomatisen
dc.subject.meshGelsen
dc.subject.meshGlyceridesen
dc.subject.meshHIV-1en
dc.subject.meshHerpesvirus 2, Humanen
dc.subject.meshHumansen
dc.subject.meshMicrobial Sensitivity Testsen
dc.subject.meshNeisseria gonorrhoeaeen
dc.subject.meshRabbitsen
dc.subject.meshSemenen
dc.subject.meshSexually Transmitted Diseasesen
dc.titleHydrogels containing monocaprin have potent microbicidal activities against sexually transmitted viruses and bacteria in vitroen
dc.typeArticleen
dc.contributor.departmentInstitute of Biology, University of Iceland, Reykjavik, Iceland.en
dc.identifier.journalSexually transmitted infectionsen
html.description.abstractOBJECTIVE: To investigate the in vitro microbicidal and cytocidal potency of monocaprin dissolved in pharmaceutical hydrogel formulations and to evaluate their potential use as vaginal microbicides against sexually transmitted pathogens such as herpes simplex virus type 2 (HSV-2), human immunodeficiency virus type 1 (HIV-1), Chlamydia trachomatis, and Neisseria gonorrhoeae. METHODS: Gel formulations were mixed with equal volumes of virus/bacteria suspensions in culture medium and incubated for 1 and 5 minutes. The reduction in virus/bacteria titre was used as a measure of microbicidal activity. Similarly, gels were mixed with human semen to study their effect on leucocytes. The toxicity of the gels was tested in rabbits by the standard vaginal irritation test. RESULTS: Gels containing 20 mM of monocaprin caused a greater than 100,000-fold inactivation of HSV-2 and Neisseria in 1 minute and of Chlamydia in 5 minutes. Similarly, the gels caused a greater than 10,000-fold inactivation of HIV-1 in semen in 1 minute. They caused more than a 10,000-fold reduction in the number of viable leucocytes in semen in 1 minute. No toxic effect on the vaginal mucosa of rabbits was observed after daily exposure for 10 days. CONCLUSIONS: Hydrogels containing monocaprin are potent inactivators of sexually transmitted viruses and bacteria in vitro. This simple lipid seems to be a feasible choice as a mucosal microbicide for prevention of sexually transmitted infections. It is a natural compound found in certain foodstuffs such as milk and is therefore unlikely to cause harmful side effects in the concentrations used.


This item appears in the following Collection(s)

Show simple item record