• English
    • íslenska
  • English 
    • English
    • íslenska
  • Login
View Item 
  •   Home
  • Journal Articles, Peer Reviewed (Ritrýndar vísindagreinar)
  • English Journal Articles (Peer Reviewed)
  • View Item
  •   Home
  • Journal Articles, Peer Reviewed (Ritrýndar vísindagreinar)
  • English Journal Articles (Peer Reviewed)
  • View Item
JavaScript is disabled for your browser. Some features of this site may not work without it.

Browse

All of HirslaCommunitiesAuthorsTitleSubjectsSubject (MeSH)Issue DateJournalThis CollectionAuthorsTitleSubjectsSubject (MeSH)Issue DateJournal

My Account

LoginRegister

Local Links

FAQ - (Icelandic)FAQ - (English)Hirsla LogosAbout LandspitaliLSH Home PageLibrary HomeIcelandic Journals

Statistics

Display statistics

Isolation of DNA for PCR assays from noncultivable Campylobacter jejuni isolates.

  • CSV
  • RefMan
  • EndNote
  • BibTex
  • RefWorks
Average rating
 
   votes
Cast your vote
You can rate an item by clicking the amount of stars they wish to award to this item. When enough users have cast their vote on this item, the average rating will also be shown.
Star rating
 
Your vote was cast
Thank you for your feedback
Authors
Callicott, K A
Stern, N J
Hiett, K L
Briem, H
Fridriksdottir, V
Georgsson, F
Gunnarsson, E
Harðardottir, H
Kristinsson, K
Reiersen, J
Berndtson, E
Campy-on-Ice Consortium
Show allShow less
Issue Date
2005-10-01

Metadata
Show full item record
Citation
Poult. Sci. 2005, 84(10):1530-2
Abstract
Isolates of Campylobacter jejuni shipped internationally often arrive in a noncultivable state. We describe a PCR-based methodology whereby phylogenetic information can be recovered from noncultivable C. jejuni stored in Wang's transport medium. The robustness of this methodology was initially tested using 5 previously characterized strains of C. jejuni isolated from various sources associated with poultry production. These isolates were stored in Wang's transport medium before being subjected to 1 of 5 treatments designed to render the stored cells noncultivable: prolonged storage at room temperature, prolonged incubation at 42 degrees C, multiple rounds of freezing and thawing, boiling, or contamination with Pseudomonas aeruginosa (ATCC 27853). This method resulted in DNA appropriate for PCR. An approximately 400-nucleotide amplicon from the flaA gene and an approximately 800-nucleotide amplicon from 16S rDNA were readily obtained, and a 1.5-kb section of the flaA locus was amplified from about half of the samples. These results indicate that this method may be useful for isolate typing schemes based on PCR amplification of Campylobacter DNA, including flaA short variable region (flaA SVR) sequencing, multilocus sequence typing (MLST), and flaA PCR-RFLP. By using this method, isolates unrecoverable from transport medium can still be used to provide phylogenetic information for epidemiological studies.
Description
To access publisher full text version of this article. Please click on the hyperlink in Additional Links field
Additional Links
http://ps.fass.org/cgi/content/abstract/84/10/1530
Collections
English Journal Articles (Peer Reviewed)

entitlement

Related articles

  • Molecular diversity of Campylobacter coli and C. jejuni isolated from pigs at slaughter by flaA-RFLP analysis and ribotyping.
  • Authors: Moore JE, Lanser J, Heuzenroeder M, Ratcliff RM, Millar BC, Madden RH
  • Issue date: 2002 Oct
  • Genotyping of Campylobacter jejuni using seven single-nucleotide polymorphisms in combination with flaA short variable region sequencing.
  • Authors: Price EP, Thiruvenkataswamy V, Mickan L, Unicomb L, Rios RE, Huygens F, Giffard PM
  • Issue date: 2006 Aug
  • [Evaluation of genotypic and phenotypic methods for the differentiation of Campylobacter jejuni and Campylobacter coli clinical isolates from Poland. II. PFGE, ERIC-PCR, PCR-flaA-RFLP and MLST].
  • Authors: Wardak S, Jagielski M
  • Issue date: 2009
  • Detection of genetic diversity in Campylobacter jejuni isolated from a commercial turkey flock using flaA typing, MLST analysis and microarray assay.
  • Authors: El-Adawy H, Hotzel H, Tomaso H, Neubauer H, Taboada EN, Ehricht R, Hafez HM
  • Issue date: 2013
  • Typing of Campylobacter jejuni and Campylobacter coli isolated from live broilers and retail broiler meat by flaA-RFLP, MLST, PFGE and REP-PCR.
  • Authors: Behringer M, Miller WG, Oyarzabal OA
  • Issue date: 2011 Feb

DSpace software (copyright © 2002 - 2021)  DuraSpace
Quick Guide | Contact Us
Open Repository is a service operated by 
Atmire NV
 

Export search results

The export option will allow you to export the current search results of the entered query to a file. Different formats are available for download. To export the items, click on the button corresponding with the preferred download format.

By default, clicking on the export buttons will result in a download of the allowed maximum amount of items.

To select a subset of the search results, click "Selective Export" button and make a selection of the items you want to export. The amount of items that can be exported at once is similarly restricted as the full export.

After making a selection, click one of the export format buttons. The amount of items that will be exported is indicated in the bubble next to export format.