Flow cytometric DNA index, G-band karyotyping, and comparative genomic hybridization in detection of high hyperdiploidy in childhood acute lymphoblastic leukemia
Cast your vote
You can rate an item by clicking the amount of stars they wish to award to this item.
When enough users have cast their vote on this item, the average rating will also be shown.
Your vote was cast
Thank you for your feedback
Thank you for your feedback
Kristensen, Tim D
Jonsson, Olafur G
Carlsen, Niels T
Larsen, Jørgen K
Christensen, Ib J
MetadataShow full item record
CitationJ. Pediatr. Hematol. Oncol. 2006, 28(3):134-40
AbstractHigh hyperdiploid acute lymphoblastic leukemia in children is related to a good outcome. Because these patients may be stratified to a low-intensity treatment, we have investigated the sensitivity of flow cytometry (FCM), G-band karyotyping (GBK), and high-resolution comparative genomic hybridization (HR-CGH) in detecting high hyperdiploid leukemic clones. Twenty-six girls and 34 boys with acute lymphoblastic leukemia diagnosed in 1998 to 1999 were analyzed by FCM, GBK, and HR-CGH. The correlations between DNA indices obtained by FCM, GBK, and HR-CGH were significant (rs=0.61 to 0.77; P<0.001 for all comparisons). However, in 4 of 18 patients, high hyperdiploidy was overlooked by GBK or HR-CGH, and even when FCM was applied, 2 of 18 patients with high hyperdiploidy by GBK and/or HR-CGH were classified as nonhigh hyperdiploid. If high hyperdiploid subclones were included, FCM could detect all high hyperdiploid patients found by either GBK or HR-CGH, but would then in addition classify 15% to 20% of the remaining patients as high hyperdiploid. Thus, both GBK and HR-CGH overlook patients with high hyperdiploidy, and FCM only detects all high hyperdiploid patients if small high hyperdiploid clones are included. In addition, FCM detects patients with high hyperdiploid subclones, not detected by either GBK or HR-CGH, and the challenge remains to determine the prognosis of patients with such high hyperdiploid subclones.
DescriptionTo access publisher full text version of this article. Please click on the hyperlink in Additional Links field
- Comparative genomic hybridization in pediatric acute lymphoblastic leukemia.
- Authors: Rice M, Breen CJ, O'Meara A, Breatnach F, O'Marcaigh AS, Stallings RL
- Issue date: 2000 Mar
- Genetic aberrations in pediatric acute lymphoblastic leukemia by comparative genomic hybridization.
- Authors: Karhu R, Siitonen S, Tanner M, Keinänen M, Mäkipernaa A, Lehtinen M, Vilpo JA, Isola J
- Issue date: 1997 Jun
- Detection of chromosome over- and underrepresentations in hyperdiploid acute lymphoblastic leukemia by comparative genomic hybridization.
- Authors: Wong N, Chen SJ, Cao Q, Su XY, Niu C, Wu QW, Leung TW, Wickham N, Johnson PJ, Chen Z
- Issue date: 1998 May
- Prognostic value of structural chromosomal rearrangements and small cell clones with high hyperdiploidy in children with acute lymphoblastic leukemia.
- Authors: Zemanova Z, Michalova K, Sindelarova L, Smisek P, Brezinova J, Ransdorfova S, Vavra V, Dohnalova A, Stary J
- Issue date: 2005 Mar
- Diagnostic and prognostic significance of chromosome abnormalities in childhood acute lymphoblastic leukemia.
- Authors: Oláh E, Balogh E, Kajtár P, Pajor L, Jakab Z, Kiss C
- Issue date: 1997 Sep 17