• Ventilator-induced lung-injury in mouse models: Is there a trap?

      Joelsson, Jon Petur; Ingthorsson, Saevar; Kricker, Jennifer; Gudjonsson, Thorarinn; Karason, Sigurbergur; 1Stem Cell Research Unit, BioMedical Center, School of Health Sciences, University of Iceland, Reykjavik, Iceland. jpj@hi.is. 2Department of Laboratory Hematology, Landspitali-University Hospital, Reykjavik, Iceland. jpj@hi.is. 3EpiEndo Pharmaceuticals, Seltjarnarnes, Iceland. jpj@hi.is. 4Department of Laboratory Hematology, Landspitali-University Hospital, Reykjavik, Iceland. 5Faculty of Nursing, University of Iceland, Reykjavik, Iceland. 6EpiEndo Pharmaceuticals, Seltjarnarnes, Iceland. 7Stem Cell Research Unit, BioMedical Center, School of Health Sciences, University of Iceland, Reykjavik, Iceland. 8Intensive Care Unit, Landspitali-University Hospital, Reykjavik, Iceland. (BioMed Central, 2021-10-29)
      Ventilator-induced lung injury (VILI) is a serious acute injury to the lung tissue that can develop during mechanical ventilation of patients. Due to the mechanical strain of ventilation, damage can occur in the bronchiolar and alveolar epithelium resulting in a cascade of events that may be fatal to the patients. Patients requiring mechanical ventilation are often critically ill, which limits the possibility of obtaining patient samples, making VILI research challenging. In vitro models are very important for VILI research, but the complexity of the cellular interactions in multi-organ animals, necessitates in vivo studies where the mouse model is a common choice. However, the settings and duration of ventilation used to create VILI in mice vary greatly, causing uncertainty in interpretation and comparison of results. This review examines approaches to induce VILI in mouse models for the last 10 years, to our best knowledge, summarizing methods and key parameters presented across the studies. The results imply that a more standardized approach is warranted. Keywords: Acute lung injury; Animal models; Mouse studies; Ventilator-induced lung injury.