• Major neonatal surgery under local anesthesia: a cohort study from Bangladesh.

      Hagander, L; Kabir, M; Chowdhury, Md Z; Gunnarsdóttir, A; Habib, Md G; Banu, T; [ 1 ] Childrens Hosp, Dept Pediat Surg, Lund, Sweden [ 2 ] Lund Univ, Dept Clin Sci, Fac Med, Lund, Sweden [ 3 ] Harvard Univ, Sch Med, Program Global Surg & Social Change, Dept Global Hlth & Social Med, Boston, MA USA [ 4 ] Chittagong Med Coll & Hosp, Dept Pediat Surg, Chittagong 4000, Bangladesh [ 5 ] Karolinska Univ Hosp, Dept Pediat Surg, Astrid Lindgren Children Hosp, Stockholm, Sweden [ 6 ] Landspitali Univ Hosp, Dept Pediat Surg, Children Hosp Hringurinn, Reykjavik, Iceland (Springer, 2015-04)
      Surgeons and anesthetists must respond to the perioperative mortality associated with general anesthesia in developing countries. The safety of performing major neonatal surgery under local anesthesia is one pragmatic response. This study describes and evaluates such practice in a tertiary pediatric surgery center in Bangladesh.
    • Outcome of patients with esophageal perforations: a multicenter study.

      Biancari, Fausto; Saarnio, Juha; Mennander, Ari; Hypén, Linda; Salminen, Paulina; Kuttila, Kari; Victorzon, Mikael; Böckelman, Camilla; Tarantino, Enrico; Tiffet, Olivier; et al. (Springer, 2014-04)
      Recent studies have suggested that stent-grafting may improve the treatment outcome of patients with esophageal perforation, but evidence on this is still lacking.
    • Tissue MicroArray analyses of pancreatic duodenal homeobox-1 in human cancers

      Wang, Xiao-Ping; Li, Zhi-Jun; Magnusson, Jonas; Brunicardi, F Charles (Springer Verlag, 2005-03-01)
      In previous studies, we demonstrated that rat insulin promoter (RIP)-driven gene therapy successfully targeted human pancreatic tumor PANC-1 cells and mouse insulinoma NIT-1 cells, which are both pancreatic duodenal homeobox-1 (PDX-1)-positive. The purpose of this study was to perform a human tissue array analysis to determine potential targets for RIP-driven gene therapy. A custom-designed tissue MicroArray analysis of various human cancer specimens was performed using a PDX-1 polyclonal antibody generated in our laboratory. The custom-designed Tissue MicroArray of human tumor specimens consists of human cancer specimens from different origins, such as the pancreas, breast, colon, prostate, kidney, liver, lung, and ovary. A panel of normal human specimens from 20 organs or tissues was used as a control. All tissues were fixed in formalin and embedded in paraffin. The immunohistochemistry studies of the cytoplasm and the nuclear expression levels were compared using the Loda method and blind reviews. Data are presented as the mean +/- SEM (p < 0.05 was considered significant by the unpaired student t-test). PDX-1 expression intensity was elevated in both benign and malignant tissues from the same patient with pancreas, breast, colon, prostate, and kidney cancers, whereas normal human tissues from control subjects without cancer did not express PDX-1. These results suggest that PDX-1 is an early marker for these cancers and could be potentially used as a diagnostic parameter and perhaps could be targeted by PDX-1-activated gene therapies, such as RIP-TK.