Very-low-density lipoprotein of uremic patients is a poor substrate for bovine lipoprotein lipase in vitro
Name:
Publisher version
View Source
Access full-text PDFOpen Access
View Source
Check access options
Check access options
Average rating
Cast your vote
You can rate an item by clicking the amount of stars they wish to award to this item.
When enough users have cast their vote on this item, the average rating will also be shown.
Star rating
Your vote was cast
Thank you for your feedback
Thank you for your feedback
Issue Date
1996-06-01
Metadata
Show full item recordCitation
Metab. Clin. Exp. 1996, 45(6):686-90Abstract
Very-low-density lipoprotein (VLDL) from 10 hemodialysis patients and 10 healthy controls was studied with respect to the substrate characteristics for bovine milk lipoprotein lipase (LPL). Compared with the control subjects, the hemodialysis patients had significantly higher serum triglyceride and apolipoprotein B-associated apolipoprotein CIII concentrations (1.03 +/- 0.31 v 1.98 +/- 0.86 mmol/L and 0.004 +/- 0.002 v 0.011 +/- 0.005 g/L, respectively), lower serum high-density lipoprotein (HDL) cholesterol and apolipoprotein AI concentrations (1.33 +/- 0.37 v 0.95 +/- 0.31 mmol/L and 1.29 +/- 0.25 v 1.09 +/- 0.23 g/L, respectively), and lower postheparin plasma LPL activity (82 +/- 24 v 35 +/- 14 milliU/milliL). There were also significant increases in the relative fat content and diameter of VLDL particles from patients versus controls. VLDL was labeled with a fluorescent phospholipid analog, DHPE, and the rate of the lipolytic reaction with purified bovine milk LPL was estimated from the increase in fluorescence intensity at 490 nm. There was no significant difference between initial reaction velocities in the study groups, but VLDL particles from hemodialysis patients were lipolyzed to a significantly lesser extent than those from healthy controls (mean increase in fluorescence intensity after completion of the reaction, 95 +/- 36 v 140 +/- 43 arbitrary units). These results are in accordance with the accumulation of remnant particles reported to occur in uremia despite only a moderately increased serum triglyceride concentration.Description
To access publisher full text version of this article. Please click on the hyperlink in Additional Links fieldAdditional Links
http://dx.doi.org/10.1016/S0026-0495(96)90132-8ae974a485f413a2113503eed53cd6c53
10.1016/S0026-0495(96)90132-8
Scopus Count
Collections
Related articles
- Lipolytic degradation of human very low density lipoproteins by human milk lipoprotein lipase: the identification of lipoprotein B as the main lipoprotein degradation product.
- Authors: Alaupovic P, Wang CS, McConathy WJ, Weiser D, Downs D
- Issue date: 1986 Jan
- Influence of lipoprotein lipase and hepatic lipase on the transformation of VLDL and HDL during lipolysis of VLDL.
- Authors: Murdoch SJ, Breckenridge WC
- Issue date: 1995 Dec
- Kinetics of in vitro lipolysis of human very low-density lipoprotein by lipoprotein lipase.
- Authors: Schreier L, Berg G, Zago V, Gonzalez AI, Wikinski R
- Issue date: 2002 Feb
- Inhibition of lipoprotein lipase induced cholesterol ester accumulation in human hepatoma HepG2 cells.
- Authors: Cianflone K, Avramoglu RK, Sawyez C, Huff MW
- Issue date: 1996 Feb
- Metabolism of apolipoprotein B-100 and of triglyceride-rich lipoprotein particles in the absence of functional lipoprotein lipase.
- Authors: Pacy PJ, Mitropoulos KA, Venkatesan S, Watts GF, Reeves BE, Halliday D
- Issue date: 1993 Nov