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dc.contributor.authorArnadottir, M
dc.contributor.authorDallongeville, J
dc.contributor.authorFruchart, J C
dc.contributor.authorNilsson-Ehle, P
dc.date.accessioned2010-10-01T13:28:59Z
dc.date.available2010-10-01T13:28:59Z
dc.date.issued1996-06-01
dc.date.submitted2010-10-01
dc.identifier.citationMetab. Clin. Exp. 1996, 45(6):686-90en
dc.identifier.issn0026-0495
dc.identifier.pmid8637441
dc.identifier.doi10.1016/S0026-0495(96)90132-8
dc.identifier.urihttp://hdl.handle.net/2336/112238
dc.descriptionTo access publisher full text version of this article. Please click on the hyperlink in Additional Links fielden
dc.description.abstractVery-low-density lipoprotein (VLDL) from 10 hemodialysis patients and 10 healthy controls was studied with respect to the substrate characteristics for bovine milk lipoprotein lipase (LPL). Compared with the control subjects, the hemodialysis patients had significantly higher serum triglyceride and apolipoprotein B-associated apolipoprotein CIII concentrations (1.03 +/- 0.31 v 1.98 +/- 0.86 mmol/L and 0.004 +/- 0.002 v 0.011 +/- 0.005 g/L, respectively), lower serum high-density lipoprotein (HDL) cholesterol and apolipoprotein AI concentrations (1.33 +/- 0.37 v 0.95 +/- 0.31 mmol/L and 1.29 +/- 0.25 v 1.09 +/- 0.23 g/L, respectively), and lower postheparin plasma LPL activity (82 +/- 24 v 35 +/- 14 milliU/milliL). There were also significant increases in the relative fat content and diameter of VLDL particles from patients versus controls. VLDL was labeled with a fluorescent phospholipid analog, DHPE, and the rate of the lipolytic reaction with purified bovine milk LPL was estimated from the increase in fluorescence intensity at 490 nm. There was no significant difference between initial reaction velocities in the study groups, but VLDL particles from hemodialysis patients were lipolyzed to a significantly lesser extent than those from healthy controls (mean increase in fluorescence intensity after completion of the reaction, 95 +/- 36 v 140 +/- 43 arbitrary units). These results are in accordance with the accumulation of remnant particles reported to occur in uremia despite only a moderately increased serum triglyceride concentration.
dc.language.isoenen
dc.publisherSaundersen
dc.relation.urlhttp://dx.doi.org/10.1016/S0026-0495(96)90132-8en
dc.subject.meshAdolescenten
dc.subject.meshAgeden
dc.subject.meshAnimalsen
dc.subject.meshCattleen
dc.subject.meshFemaleen
dc.subject.meshFluorescent Dyesen
dc.subject.meshHumansen
dc.subject.meshLipolysisen
dc.subject.meshLipoprotein Lipaseen
dc.subject.meshLipoproteins, VLDLen
dc.subject.meshMaleen
dc.subject.meshMiddle Ageden
dc.subject.meshRenal Dialysisen
dc.subject.meshSubstrate Specificityen
dc.subject.meshTriglyceridesen
dc.subject.meshUremiaen
dc.titleVery-low-density lipoprotein of uremic patients is a poor substrate for bovine lipoprotein lipase in vitroen
dc.typeArticleen
dc.contributor.departmentDepartment of Nephrology, University Hospital, Lund, Sweden.en
dc.identifier.journalMetabolism: clinical and experimentalen
html.description.abstractVery-low-density lipoprotein (VLDL) from 10 hemodialysis patients and 10 healthy controls was studied with respect to the substrate characteristics for bovine milk lipoprotein lipase (LPL). Compared with the control subjects, the hemodialysis patients had significantly higher serum triglyceride and apolipoprotein B-associated apolipoprotein CIII concentrations (1.03 +/- 0.31 v 1.98 +/- 0.86 mmol/L and 0.004 +/- 0.002 v 0.011 +/- 0.005 g/L, respectively), lower serum high-density lipoprotein (HDL) cholesterol and apolipoprotein AI concentrations (1.33 +/- 0.37 v 0.95 +/- 0.31 mmol/L and 1.29 +/- 0.25 v 1.09 +/- 0.23 g/L, respectively), and lower postheparin plasma LPL activity (82 +/- 24 v 35 +/- 14 milliU/milliL). There were also significant increases in the relative fat content and diameter of VLDL particles from patients versus controls. VLDL was labeled with a fluorescent phospholipid analog, DHPE, and the rate of the lipolytic reaction with purified bovine milk LPL was estimated from the increase in fluorescence intensity at 490 nm. There was no significant difference between initial reaction velocities in the study groups, but VLDL particles from hemodialysis patients were lipolyzed to a significantly lesser extent than those from healthy controls (mean increase in fluorescence intensity after completion of the reaction, 95 +/- 36 v 140 +/- 43 arbitrary units). These results are in accordance with the accumulation of remnant particles reported to occur in uremia despite only a moderately increased serum triglyceride concentration.


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