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dc.contributor.authorWiller, M
dc.contributor.authorHoffmann, L
dc.contributor.authorStyrkarsdottir, U
dc.contributor.authorEgel, R
dc.contributor.authorDavey, J
dc.contributor.authorNielsen, O
dc.date.accessioned2010-10-12T13:43:55Z
dc.date.available2010-10-12T13:43:55Z
dc.date.issued1995-09
dc.date.submitted2010-10-12
dc.identifier.citationMol. Cell. Biol. 1995, 15(9):4964-70 .en
dc.identifier.issn0270-7306
dc.identifier.pmid7651414
dc.identifier.urihttp://hdl.handle.net/2336/112802
dc.descriptionTo access publisher full text version of this article. Please click on the hyperlink in Additional Links fielden
dc.description.abstractThe mat1 locus is a key regulator of both conjugation and meiosis in the fission yeast Schizosaccharomyces pombe. Two alternative DNA segments of this locus, mat1-P and mat1-M, specify the haploid cell types (Plus and Minus). Each segment includes two genes: mat1-P includes mat1-Pc and mat1-Pm, while mat1-M includes mat1-Mc and mat1-Mm. The mat1-Pc and mat1-Mc genes are responsible for establishing the pheromone communication system that mediates conjugation between P and M cells, while all four mat1 genes are required for meiosis in diploid P/M cells. Our understanding of the initiation of meiosis is based largely on indirect observations, and a more precise investigation of these events was required to define the interaction between the mat1 genes. Here we resolve this issue using synthetic pheromones and P/M strains with mutations in either mat1-Pc or mat1-Mc. Our results suggest a model in which the mat1 locus plays two roles in controlling meiosis. In the first instance, the mat1-Pc and mat1-Mc functions are required to produce the mating pheromones and receptors that allow the generation of a pheromone signal. This signal is required to induce the expression of mat1-Pm and mat1-Mm. This appears to be the major pheromone-dependent step in controlling meiosis since ectopic expression of these genes allows meiosis in the absence of mat1-Pc and mat1-Mc. The mat1-Pm and mat1-Mm products complete the initiation of meiosis by activating transcription of the mei3 gene.
dc.language.isoenen
dc.publisherAmerican Society for Microbiologyen
dc.relation.urlhttp://mcb.asm.org/cgi/content/abstract/15/9/4964en
dc.subject.meshBase Sequenceen
dc.subject.meshFungal Proteinsen
dc.subject.meshGene Expression Regulation, Fungalen
dc.subject.meshGenes, Fungalen
dc.subject.meshMeiosisen
dc.subject.meshModels, Biologicalen
dc.subject.meshMolecular Sequence Dataen
dc.subject.meshPeptidesen
dc.subject.meshPheromonesen
dc.subject.meshReproductionen
dc.subject.meshSchizosaccharomycesen
dc.subject.meshSchizosaccharomyces pombe Proteinsen
dc.subject.meshSignal Transductionen
dc.subject.meshTranscription, Geneticen
dc.titleTwo-step activation of meiosis by the mat1 locus in Schizosaccharomyces pombeen
dc.typeArticleen
dc.contributor.departmentDepartment of Genetics, University of Copenhagen, Denmark.en
dc.identifier.journalMolecular and cellular biologyen
html.description.abstractThe mat1 locus is a key regulator of both conjugation and meiosis in the fission yeast Schizosaccharomyces pombe. Two alternative DNA segments of this locus, mat1-P and mat1-M, specify the haploid cell types (Plus and Minus). Each segment includes two genes: mat1-P includes mat1-Pc and mat1-Pm, while mat1-M includes mat1-Mc and mat1-Mm. The mat1-Pc and mat1-Mc genes are responsible for establishing the pheromone communication system that mediates conjugation between P and M cells, while all four mat1 genes are required for meiosis in diploid P/M cells. Our understanding of the initiation of meiosis is based largely on indirect observations, and a more precise investigation of these events was required to define the interaction between the mat1 genes. Here we resolve this issue using synthetic pheromones and P/M strains with mutations in either mat1-Pc or mat1-Mc. Our results suggest a model in which the mat1 locus plays two roles in controlling meiosis. In the first instance, the mat1-Pc and mat1-Mc functions are required to produce the mating pheromones and receptors that allow the generation of a pheromone signal. This signal is required to induce the expression of mat1-Pm and mat1-Mm. This appears to be the major pheromone-dependent step in controlling meiosis since ectopic expression of these genes allows meiosis in the absence of mat1-Pc and mat1-Mc. The mat1-Pm and mat1-Mm products complete the initiation of meiosis by activating transcription of the mei3 gene.


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