Cytogenetic studies of breast carcinomas: different karyotypic profiles detected by direct harvesting and short-term culture
Cast your vote
You can rate an item by clicking the amount of stars they wish to award to this item.
When enough users have cast their vote on this item, the average rating will also be shown.
Your vote was cast
Thank you for your feedback
Thank you for your feedback
MetadataShow full item record
CitationCytogenetic studies of breast carcinomas: different karyotypic profiles detected by direct harvesting and short-term culture. 1995, 13 (4):239-48 Genes Chromosomes Cancer
AbstractChromosome analysis was performed on samples from 85 consecutive patients with breast cancer by one or more of three different methods: direct harvest, culture after mechanical disaggregation, and culture after collagenase digestion. Metaphases suitable for karyotyping were obtained in 70% of the cases; direct harvest yielded metaphases in 29% and cultures without and with digestion in 40% and 59%, respectively. Chromosomal abnormalities were detected in 37 cases. Cells judged to be phenotypically abnormal in culture were twice as likely to reveal chromosomal aberrations as normal-looking cells. Eight cases showed multiclonal abnormalities. Significant differences were detected in the karyotypic profile depending on the method used. With direct harvest, the yield of complex chromosomal changes was 87%, compared to 44% after culture of digested tissue (P < 0.01), and also polyploidy was more common in direct-harvested samples. Detailed karyotypic analysis was possible in 29 primary tumors. The chromosomes most frequently involved were 1, 3, 7, 11, 16, and 17. Recurrent structural abnormalities were der(1;16)(q10;p10), i(1)(q10), del(6)(q21), and del(1)(p22). Breakpoints clustered to the centromere regions of chromosomes 1, 3, 11, 15, and 16 and to the short arms of chromosomes 7, 17, and 19. Seven of twenty-nine fully analyzed cases had a family history of breast cancer, and changes of chromosomes 1, 3, and 15 seemed to be more common in these cases. There was an association between karyotype and survival: The 3 year survival was 63% in patients with complex karyotypic changes and 92% in those without complex changes.
DescriptionTo access publisher full text version of this article. Please click on the hyperlink in Additional Links field
- Chromosome analysis of 97 primary breast carcinomas: identification of eight karyotypic subgroups.
- Authors: Pandis N, Jin Y, Gorunova L, Petersson C, Bardi G, Idvall I, Johansson B, Ingvar C, Mandahl N, Mitelman F
- Issue date: 1995 Mar
- Cytogenetic analysis of pancreatic carcinomas: intratumor heterogeneity and nonrandom pattern of chromosome aberrations.
- Authors: Gorunova L, Höglund M, Andrén-Sandberg A, Dawiskiba S, Jin Y, Mitelman F, Johansson B
- Issue date: 1998 Oct
- Assessment of methods for the cytogenetic analysis of human solid tumors.
- Authors: Tanaka K, Testa JR
- Issue date: 1987 Dec
- Karyotypic evolution in breast carcinomas with i(1)(q10) and der(1;16)(q10;p10) as the primary chromosome abnormality.
- Authors: Tsarouha H, Pandis N, Bardi G, Teixeira MR, Andersen JA, Heim S
- Issue date: 1999 Sep
- Karyotypic changes in phyllodes tumors of the breast.
- Authors: Dietrich CU, Pandis N, Bardi G, Teixeira MR, Soukhikh T, Petersson C, Andersen JA, Heim S
- Issue date: 1994 Dec