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dc.contributor.authorSteinarsdottir, M
dc.contributor.authorPetursdottir, I
dc.contributor.authorSnorradottir, S
dc.contributor.authorEyfjord, J E
dc.contributor.authorOgmundsdottir, H M
dc.date.accessioned2010-10-12T15:39:40Z
dc.date.available2010-10-12T15:39:40Z
dc.date.issued1995-08-01
dc.date.submitted2010-10-12
dc.identifier.citationCytogenetic studies of breast carcinomas: different karyotypic profiles detected by direct harvesting and short-term culture. 1995, 13 (4):239-48 Genes Chromosomes Canceren
dc.identifier.issn1045-2257
dc.identifier.pmid7547631
dc.identifier.doi10.1002/gcc.2870130403
dc.identifier.urihttp://hdl.handle.net/2336/112807
dc.descriptionTo access publisher full text version of this article. Please click on the hyperlink in Additional Links fielden
dc.description.abstractChromosome analysis was performed on samples from 85 consecutive patients with breast cancer by one or more of three different methods: direct harvest, culture after mechanical disaggregation, and culture after collagenase digestion. Metaphases suitable for karyotyping were obtained in 70% of the cases; direct harvest yielded metaphases in 29% and cultures without and with digestion in 40% and 59%, respectively. Chromosomal abnormalities were detected in 37 cases. Cells judged to be phenotypically abnormal in culture were twice as likely to reveal chromosomal aberrations as normal-looking cells. Eight cases showed multiclonal abnormalities. Significant differences were detected in the karyotypic profile depending on the method used. With direct harvest, the yield of complex chromosomal changes was 87%, compared to 44% after culture of digested tissue (P < 0.01), and also polyploidy was more common in direct-harvested samples. Detailed karyotypic analysis was possible in 29 primary tumors. The chromosomes most frequently involved were 1, 3, 7, 11, 16, and 17. Recurrent structural abnormalities were der(1;16)(q10;p10), i(1)(q10), del(6)(q21), and del(1)(p22). Breakpoints clustered to the centromere regions of chromosomes 1, 3, 11, 15, and 16 and to the short arms of chromosomes 7, 17, and 19. Seven of twenty-nine fully analyzed cases had a family history of breast cancer, and changes of chromosomes 1, 3, and 15 seemed to be more common in these cases. There was an association between karyotype and survival: The 3 year survival was 63% in patients with complex karyotypic changes and 92% in those without complex changes.
dc.language.isoenen
dc.publisherWiley-Liss Incen
dc.relation.urlhttp://dx.doi.org/10.1002/gcc.2870130403en
dc.subject.meshBreast Neoplasmsen
dc.subject.meshCase-Control Studiesen
dc.subject.meshChromosome Aberrationsen
dc.subject.meshChromosome Bandingen
dc.subject.meshChromosome Mappingen
dc.subject.meshCulture Techniquesen
dc.subject.meshFemaleen
dc.subject.meshHumansen
dc.subject.meshKaryotypingen
dc.subject.meshMetaphaseen
dc.subject.meshMiddle Ageden
dc.subject.meshMitotic Indexen
dc.subject.meshSurvival Rateen
dc.subject.meshTumor Cells, Cultureden
dc.titleCytogenetic studies of breast carcinomas: different karyotypic profiles detected by direct harvesting and short-term cultureen
dc.typeArticleen
dc.contributor.departmentDepartment of Pathology, University Hospital of Iceland, Reykjavik.en
dc.identifier.journalGenes, chromosomes & canceren
html.description.abstractChromosome analysis was performed on samples from 85 consecutive patients with breast cancer by one or more of three different methods: direct harvest, culture after mechanical disaggregation, and culture after collagenase digestion. Metaphases suitable for karyotyping were obtained in 70% of the cases; direct harvest yielded metaphases in 29% and cultures without and with digestion in 40% and 59%, respectively. Chromosomal abnormalities were detected in 37 cases. Cells judged to be phenotypically abnormal in culture were twice as likely to reveal chromosomal aberrations as normal-looking cells. Eight cases showed multiclonal abnormalities. Significant differences were detected in the karyotypic profile depending on the method used. With direct harvest, the yield of complex chromosomal changes was 87%, compared to 44% after culture of digested tissue (P < 0.01), and also polyploidy was more common in direct-harvested samples. Detailed karyotypic analysis was possible in 29 primary tumors. The chromosomes most frequently involved were 1, 3, 7, 11, 16, and 17. Recurrent structural abnormalities were der(1;16)(q10;p10), i(1)(q10), del(6)(q21), and del(1)(p22). Breakpoints clustered to the centromere regions of chromosomes 1, 3, 11, 15, and 16 and to the short arms of chromosomes 7, 17, and 19. Seven of twenty-nine fully analyzed cases had a family history of breast cancer, and changes of chromosomes 1, 3, and 15 seemed to be more common in these cases. There was an association between karyotype and survival: The 3 year survival was 63% in patients with complex karyotypic changes and 92% in those without complex changes.


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