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Molecular genetics and cytogenetics of breast carcinomas: comparison of the two methods

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Authors
Valgardsdottir, R
Steinarsdottir, M
Anamthawat-Jonsson, K
Petursdottir, I
Ogmundsdottir, H M
Eyfjord, J E
Issue Date
1996-11

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Cancer Genet. Cytogenet. 1996, 92(1):37-42
Abstract
Molecular genetics and cytogenetics are two different approaches to studying genetic changes in breast carcinoma. We have used karyotype analysis, fluorescence in situ hybridization, and molecular analysis of allelic imbalance on chromosomes 7q and 16q and on both arms of chromosome 17, to study 85 breast carcinomas. Twenty-five of these samples gave results that could be used to compare the two methods. Sixty-nine chromosome arms were compared, of which 48 (70%) gave concordant molecular and cytogenetical results. Samples were processed for karyotyping both by harvesting directly from the fresh tissue and after selective culture for a few days. Karyotypes among the direct harvest samples matched significantly better with the molecular genetics results than karyotypes among the cultured cell preparations.
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http://dx.doi.org/10.1016/S0165-4608(96)00150-1
ae974a485f413a2113503eed53cd6c53
10.1016/S0165-4608(96)00150-1
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English Journal Articles (Peer Reviewed)

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