A discrepancy between liquid phase and gel phase assays for evaluation of total complement activity and some possible explanations
Cast your vote
You can rate an item by clicking the amount of stars they wish to award to this item.
When enough users have cast their vote on this item, the average rating will also be shown.
Your vote was cast
Thank you for your feedback
Thank you for your feedback
MetadataShow full item record
CitationScand. J. Immunol. 2004, 59(6):574-81
AbstractThis study was conducted to investigate the frequency and origin of discrepant assay results between two haemolytic assays which both measure activity of the classical pathway of complement (CH50) by haemolysis of sheep red blood cells (SRBCs). One is conducted in gel phase using undiluted sera and the other in liquid phase with sera in 1/100 dilution. The majority of discrepant readings are observed as low or absent haemolysis in the gel phase, with values within or above the normal range in the liquid phase. The incidence of discrepant assay readings was evaluated in 300 samples. Furthermore, 28 samples showing the most discrepant readings were investigated further for disturbing factors. Factors evaluated in the test sera were mannose-binding lectin, C-reactive protein (CRP), immune complexes, antibodies to SRBCs, rheumatoid factor and immunoglobulin A (IgA) and IgG anti-C1q antibodies. The results showed that discrepant readings are present in 10% of the 300 samples and false low gel assay readings account for 6.3%. The majority (68%) of the discrepant samples contained a heat-stable-inhibiting factor, and the main mediators found were elevated levels of IgA anti-C1q antibodies and antibodies to SRBCs. This could indicate a clinically relevant factor in the test sera but can also result from the difference in assay design.
DescriptionTo access publisher full text version of this article. Please click on the hyperlink in Additional Link field
- Reactivity of low molecular weight material in cellular immune complex assays.
- Authors: Cooper KM, Moore M
- Issue date: 1983 May
- Solid-phase classical complement activation by C-reactive protein (CRP) is inhibited by fluid-phase CRP-C1q interaction.
- Authors: Sjöwall C, Wetterö J, Bengtsson T, Askendal A, Almroth G, Skogh T, Tengvall P
- Issue date: 2007 Jan 5
- Depletion of total hemolytic complement in sera from hamsters bearing herpes simplex type 2-induced tumors.
- Authors: Márquez ED, Backenstose JM, Chapman JL
- Issue date: 1980 Mar
- Complexes between C1q and C3 or C4: novel and specific markers for classical complement pathway activation.
- Authors: Wouters D, Wiessenberg HD, Hart M, Bruins P, Voskuyl A, Daha MR, Hack CE
- Issue date: 2005 Mar
- Pathway-specific complement activity in pigs evaluated with a human functional complement assay.
- Authors: Salvesen B, Mollnes TE
- Issue date: 2009 May