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dc.contributor.authorArnardottir, Hildur H
dc.contributor.authorFreysdottir, Jona
dc.contributor.authorHardardottir, Ingibjorg
dc.date.accessioned2013-07-02T11:24:31Z
dc.date.available2013-07-02T11:24:31Z
dc.date.issued2012-09
dc.date.submitted2013-07-02
dc.identifier.citationInflamm. Res. 2012, 61(9):931-9en_GB
dc.identifier.issn1420-908X
dc.identifier.pmid22585035
dc.identifier.doi10.1007/s00011-012-0484-0
dc.identifier.urihttp://hdl.handle.net/2336/295046
dc.description.abstractRecent studies indicate that neutrophils are heterogeneous and may have an immunosuppressive role in addition to their well-known phagocytic and bactericidal function. This study examined neutrophil subpopulations in the circulation, peritoneum, spleen and bone marrow from mice at various time points after induction of acute inflammation. MATERIAL, TREATMENT AND METHODS: Female C57BL/6 mice were injected intraperitoneally with lipopolysaccharide (LPS). Blood, peritoneal, spleen and bone marrow cells were collected and counted and expression of surface molecules and chemokine receptors analyzed with flow cytometry. Chemokine and cytokine concentrations in serum and peritoneal fluid were determined by ELISA. Neutrophil numbers in the circulation decreased following administration of LPS but reached similar numbers to those prior to inflammation at 8 h. At that time point, two distinct neutrophil populations were present in the circulation. These two neutrophil populations differed in size, granularity and expression of CD11b and Ly6G. Few neutrophils were recruited into the peritoneum until 24 h after administration of LPS at a time when the neutrophils in the circulation had increased their expression of the chemokine receptor CXCR2. Induction of acute inflammation leads to the appearance of two circulating neutrophil subpopulations, which may differ in their activation state and function.
dc.description.sponsorshipIcelandic Research Fund University of Iceland Research Funden_GB
dc.language.isoenen
dc.publisherSpringeren_GB
dc.relation.urlhttp:dx.doi.org/10.1007/s00011-012-0484-0en_GB
dc.rightsArchived with thanks to Inflammation research : official journal of the European Histamine Research Society ... [et al.]en_GB
dc.subject.meshAnimalsen_GB
dc.subject.meshAscitic Fluiden_GB
dc.subject.meshCell Sizeen_GB
dc.subject.meshCytokinesen_GB
dc.subject.meshFemaleen_GB
dc.subject.meshInflammationen_GB
dc.subject.meshLeukocyte Counten_GB
dc.subject.meshLipopolysaccharidesen_GB
dc.subject.meshMiceen_GB
dc.subject.meshMice, Inbred C57BLen_GB
dc.subject.meshNeutrophilsen_GB
dc.titleTwo circulating neutrophil populations in acute inflammation in mice.en
dc.typeArticleen
dc.contributor.departmentDepartment of Biochemistry and Molecular Biology, University of Iceland, Vatnsmyrarvegur 16, IS-101, Reykjavik, Iceland.en_GB
dc.identifier.journalInflammation research : official journal of the European Histamine Research Society ... [et al.]en_GB
dc.rights.accessNational Consortium - Landsaðganguren
html.description.abstractRecent studies indicate that neutrophils are heterogeneous and may have an immunosuppressive role in addition to their well-known phagocytic and bactericidal function. This study examined neutrophil subpopulations in the circulation, peritoneum, spleen and bone marrow from mice at various time points after induction of acute inflammation. MATERIAL, TREATMENT AND METHODS: Female C57BL/6 mice were injected intraperitoneally with lipopolysaccharide (LPS). Blood, peritoneal, spleen and bone marrow cells were collected and counted and expression of surface molecules and chemokine receptors analyzed with flow cytometry. Chemokine and cytokine concentrations in serum and peritoneal fluid were determined by ELISA. Neutrophil numbers in the circulation decreased following administration of LPS but reached similar numbers to those prior to inflammation at 8 h. At that time point, two distinct neutrophil populations were present in the circulation. These two neutrophil populations differed in size, granularity and expression of CD11b and Ly6G. Few neutrophils were recruited into the peritoneum until 24 h after administration of LPS at a time when the neutrophils in the circulation had increased their expression of the chemokine receptor CXCR2. Induction of acute inflammation leads to the appearance of two circulating neutrophil subpopulations, which may differ in their activation state and function.


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