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dc.contributor.authorCalla-Magariños, Jacqueline
dc.contributor.authorFernández, Carmen
dc.contributor.authorTroye-Blomberg, Marita
dc.contributor.authorFreysdottir, Jona
dc.date.accessioned2014-02-12T14:11:50Z
dc.date.available2014-02-12T14:11:50Z
dc.date.issued2013-05
dc.date.submitted2013
dc.identifier.citationInt. Immunopharmacol. 2013, 16 (1):79-84en
dc.identifier.issn1878-1705
dc.identifier.pmid23562757
dc.identifier.doi10.1016/j.intimp.2013.03.022
dc.identifier.urihttp://hdl.handle.net/2336/312688
dc.descriptionTo access publisher's full text version of this article. Please click on the hyperlink in Additional Links field.en
dc.description.abstractAlkaloids obtained from the plant Evanta have been shown to have dual effects in Leishmania infection; a direct leishmanicidal effect on the parasite and more importantly, the alkaloids affect both polyclonal and Leishmania-specific stimulation of T-cells. Dendritic cells (DCs) play a pivotal role in stimulation and polarization of naïve T cells towards a Th1, Th2, Th17 or regulatory phenotype. In leishmaniasis, the interactions between the parasites and DCs are complex and involve contradictory functions that can stimulate or suppress T cell responses, leading to the control of infection or progression of disease. In this study the effect of an alkaloid extract of Evanta (AEE) or the purified alkaloid 2-phenilquinoline (2Ph) on the activation of human DCs and their ability to stimulate allogeneic CD4(+) T cells was analyzed. The expression of surface activation molecules was not affected on DCs stimulated in the presence of AEE or 2Ph nor did AEE-DCs or 2Ph-CDs affect the expression of activation surface molecules on allogeneic CD4(+) T cells. In contrast, as compared with control, the secretion of IL-12p40, IL-23 and IL-6 was lower from AEE-DCs and 2Ph-CDs and allogeneic CD4(+) T cells co-cultured with these DCs secreted lower levels of IFN-γ and IL-10 but the same levels of IL-17. These results demonstrate that AEE and 2Ph affect the stimulation of DCs and their ability to stimulate allogeneic CD4(+) T cells by reducing the production of IFN-γ, IL-12 p40, IL-6 and IL-23. This suggests that AEE and 2Ph may take part in regulation of inflammation.
dc.description.sponsorshipSwedish International Development Cooperation Agency (SIDA)en
dc.language.isoenen
dc.publisherElsevier Science BVen
dc.relation.urlhttp://dx.doi.org/10.1016/j.intimp.2013.03.022en
dc.rightsArchived with thanks to International immunopharmacologyen
dc.subjectFrumuskiptingen
dc.subjectFrumurannsókniren
dc.subject.meshAlkaloidsen
dc.subject.meshCD4-Positive T-Lymphocytesen
dc.subject.meshCell Differentiationen
dc.subject.meshCells, Cultureden
dc.subject.meshCoculture Techniquesen
dc.subject.meshCytokinesen
dc.subject.meshDendritic Cellsen
dc.subject.meshHumansen
dc.subject.meshImmunologic Factorsen
dc.subject.meshRutaceaeen
dc.titleAlkaloids from Galipea longiflora Krause modify the maturation of human dendritic cells and their ability to stimulate allogeneic CD4+ T cells.en
dc.typeArticleen
dc.contributor.departmentNatl Univ Hosp Iceland, Dept Immunol, Landspitali, Reykjavik, Iceland, Natl Univ Hosp Iceland, Ctr Rheumatol Res, Landspitali, Reykjavik, Iceland, Stockholm Univ, Wenner Gren Inst, Dept Immunol, S-10691 Stockholm, Sweden, Univ Mayor San Andres, SELADIS Inst, Immunol Lab, Biochem & Pharm Fac, La Paz, Bolivia, Univ Iceland, Biomed Ctr, Fac Med, Reykjavik, Icelanden
dc.identifier.journalInternational immunopharmacologyen
dc.rights.accessNational Consortium - Landsaðganguren
html.description.abstractAlkaloids obtained from the plant Evanta have been shown to have dual effects in Leishmania infection; a direct leishmanicidal effect on the parasite and more importantly, the alkaloids affect both polyclonal and Leishmania-specific stimulation of T-cells. Dendritic cells (DCs) play a pivotal role in stimulation and polarization of naïve T cells towards a Th1, Th2, Th17 or regulatory phenotype. In leishmaniasis, the interactions between the parasites and DCs are complex and involve contradictory functions that can stimulate or suppress T cell responses, leading to the control of infection or progression of disease. In this study the effect of an alkaloid extract of Evanta (AEE) or the purified alkaloid 2-phenilquinoline (2Ph) on the activation of human DCs and their ability to stimulate allogeneic CD4(+) T cells was analyzed. The expression of surface activation molecules was not affected on DCs stimulated in the presence of AEE or 2Ph nor did AEE-DCs or 2Ph-CDs affect the expression of activation surface molecules on allogeneic CD4(+) T cells. In contrast, as compared with control, the secretion of IL-12p40, IL-23 and IL-6 was lower from AEE-DCs and 2Ph-CDs and allogeneic CD4(+) T cells co-cultured with these DCs secreted lower levels of IFN-γ and IL-10 but the same levels of IL-17. These results demonstrate that AEE and 2Ph affect the stimulation of DCs and their ability to stimulate allogeneic CD4(+) T cells by reducing the production of IFN-γ, IL-12 p40, IL-6 and IL-23. This suggests that AEE and 2Ph may take part in regulation of inflammation.


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