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dc.contributor.authorBjarnarson, Stefania P
dc.contributor.authorBenonisson, Hreinn
dc.contributor.authorDel Giudice, Giuseppe
dc.contributor.authorJonsdottir, Ingileif
dc.date.accessioned2014-05-16T09:53:04Z
dc.date.available2014-05-16T09:53:04Z
dc.date.issued2013
dc.date.submitted2013
dc.identifier.citationPLoS ONE 2013, 8 (9):e72588en
dc.identifier.issn1932-6203
dc.identifier.pmid24069152
dc.identifier.doi10.1371/journal.pone.0072588
dc.identifier.urihttp://hdl.handle.net/2336/317070
dc.descriptionTo access publisher's full text version of this article, please click on the hyperlink in Additional Links field or click on the hyperlink at the top of the page marked Files. This article is open access.en
dc.description.abstractPlain pneumococcal polysaccharide (PPS) booster administered during second year of life has been shown to cause hyporesponsiveness. We assessed the effects of PPS booster on splenic memory B cell responses and persistence of PPS-specific long-lived plasma cells in the bone marrow (BM).
dc.description.abstractNeonatal mice were primed subcutanously (s.c.) or intranasally (i.n.) with pneumococcal conjugate (Pnc1-TT) and the adjuvant LT-K63, and boosted with PPS+LT-K63 or saline 1, 2 or 3 times with 16 day intervals. Seven days after each booster, spleens were removed, germinal centers (GC), IgM(+), IgG(+) follicles and PPS-specific antibody secreting cells (AbSC) in spleen and BM enumerated.
dc.description.abstractPPS booster s.c., but not i.n., compromised the Pnc1-TT-induced PPS-specific Abs by abrogating the Pnc1-TT-induced GC reaction and depleting PPS-specific AbSCs in spleen and limiting their homing to the BM. There was no difference in the frequency of PPS-specific AbSCs in spleen and BM between mice that received 1, 2 or 3 PPS boosters s.c.. Repeated PPS+LT-K63 booster i.n. reduced the frequency of PPS-specific IgG(+) AbSCs in BM.
dc.description.abstractPPS booster-induced hyporesponsiveness is caused by abrogation of conjugate-induced GC reaction and depletion of PPS-specific IgG(+) AbSCs resulting in no homing of new PPS-specific long-lived plasma cells to the BM or survival. These results should be taken into account in design of vaccination schedules where polysaccharides are being considered.
dc.description.sponsorshipIcelandic Research Fund for Graduate Students/ 50940005, Icelandic Research Fund/40438021-23, Eimskip University Fund, University of Iceland Research Fund, Landspitali University Hospital Research Funden
dc.language.isoenen
dc.publisherPublic Library Scienceen
dc.relation.urlhttp://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0072588en
dc.rightsopenAccessen
dc.subjectSýkingaren
dc.subjectBólusetningaren
dc.subjectLungnabólgaen
dc.subjectTilraunir á dýrumen
dc.subject.meshAnimalsen
dc.subject.meshAntibody-Producing Cellsen
dc.subject.meshBone Marrowen
dc.subject.meshEnzyme-Linked Immunosorbent Assayen
dc.subject.meshGerminal Centeren
dc.subject.meshImmunohistochemistryen
dc.subject.meshMiceen
dc.subject.meshPneumococcal Vaccinesen
dc.subject.meshSpleenen
dc.subject.meshStreptococcus pneumoniaeen
dc.titlePneumococcal polysaccharide abrogates conjugate-induced germinal center reaction and depletes antibody secreting cell pool, causing hyporesponsiveness.en
dc.typeArticleen
dc.contributor.departmentNatl Univ Hosp Iceland, Landspitali, Dept Immunol, Reykjavik, Iceland, Univ Iceland, Fac Med, Reykjavik, Iceland, Novartis Vaccines & Diagnost, Siena, Italy, DeCODE Genet, Reykjavik, Icelanden
dc.identifier.journalPloS oneen
dc.rights.accessOpen Accessen
refterms.dateFOA2018-09-12T13:18:50Z
html.description.abstractPlain pneumococcal polysaccharide (PPS) booster administered during second year of life has been shown to cause hyporesponsiveness. We assessed the effects of PPS booster on splenic memory B cell responses and persistence of PPS-specific long-lived plasma cells in the bone marrow (BM).
html.description.abstractNeonatal mice were primed subcutanously (s.c.) or intranasally (i.n.) with pneumococcal conjugate (Pnc1-TT) and the adjuvant LT-K63, and boosted with PPS+LT-K63 or saline 1, 2 or 3 times with 16 day intervals. Seven days after each booster, spleens were removed, germinal centers (GC), IgM(+), IgG(+) follicles and PPS-specific antibody secreting cells (AbSC) in spleen and BM enumerated.
html.description.abstractPPS booster s.c., but not i.n., compromised the Pnc1-TT-induced PPS-specific Abs by abrogating the Pnc1-TT-induced GC reaction and depleting PPS-specific AbSCs in spleen and limiting their homing to the BM. There was no difference in the frequency of PPS-specific AbSCs in spleen and BM between mice that received 1, 2 or 3 PPS boosters s.c.. Repeated PPS+LT-K63 booster i.n. reduced the frequency of PPS-specific IgG(+) AbSCs in BM.
html.description.abstractPPS booster-induced hyporesponsiveness is caused by abrogation of conjugate-induced GC reaction and depletion of PPS-specific IgG(+) AbSCs resulting in no homing of new PPS-specific long-lived plasma cells to the BM or survival. These results should be taken into account in design of vaccination schedules where polysaccharides are being considered.


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