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dc.contributor.authorSjövall, Fredrik
dc.contributor.authorEhinger, Johannes K H
dc.contributor.authorMarelsson, Sigurður E
dc.contributor.authorMorota, Saori
dc.contributor.authorFrostner, Eleonor Asander
dc.contributor.authorUchino, Hiroyuki
dc.contributor.authorLundgren, Johan
dc.contributor.authorArnbjörnsson, Einar
dc.contributor.authorHansson, Magnus J
dc.contributor.authorFellman, Vineta
dc.contributor.authorElmér, Eskil
dc.date.accessioned2014-05-20T09:42:27Z
dc.date.available2014-05-20T09:42:27Z
dc.date.issued2013-01
dc.date.submitted2014-05-20
dc.identifier.citationMitochondrion 2013, 13(1):7-14en
dc.identifier.issn1872-8278
dc.identifier.pmid23164798
dc.identifier.doi10.1016/j.mito.2012.11.001
dc.identifier.urihttp://hdl.handle.net/2336/317192
dc.descriptionTo access publisher's full text version of this article. Please click on the hyperlink in Additional Links field.en
dc.description.abstractStudying whole cell preparations with intact mitochondria and respiratory complexes has a clear benefit compared to isolated or disrupted mitochondria due to the dynamic interplay between mitochondria and other cellular compartments. Platelet mitochondria have a potential to serve as a source of human viable mitochondria when studying mitochondrial physiology and pathogenic mechanisms, as well as for the diagnostics of mitochondrial diseases. The objective of the present study was to perform a detailed evaluation of platelet mitochondrial respiration using high-resolution respirometry. Further, we aimed to explore the limits of sample size and the impact of storage as well as to establish a wide range of reference data from different pediatric and adult cohorts. Our results indicate that platelet mitochondria are well suited for ex-vivo analysis with the need for minute sample amounts and excellent reproducibility and stability.
dc.description.sponsorshipSwedish Research Council 2011-3470 Royal Physiographic Society Foundation of the Swedish National Board of Health and Welfare Carl og Ellen Hertz' legat til Dansk laege- og naturvidenskab Lippman foundationen
dc.language.isoenen
dc.publisherElsevier Scienceen
dc.relation.urlhttp://dx.doi.org/10.1016/j.mito.2012.11.001en
dc.rightsArchived with thanks to Mitochondrionen
dc.subject.meshAdulten
dc.subject.meshAge Factorsen
dc.subject.meshAgeden
dc.subject.meshBlood Plateletsen
dc.subject.meshCell Respirationen
dc.subject.meshChilden
dc.subject.meshChild, Preschoolen
dc.subject.meshCytological Techniquesen
dc.subject.meshFemaleen
dc.subject.meshHumansen
dc.subject.meshInfanten
dc.subject.meshMaleen
dc.subject.meshMiddle Ageden
dc.subject.meshMitochondriaen
dc.subject.meshSex Factorsen
dc.subject.meshSpecimen Handlingen
dc.subject.meshYoung Adulten
dc.titleMitochondrial respiration in human viable platelets--methodology and influence of gender, age and storage.en
dc.typeArticleen
dc.contributor.departmentLund Univ, Mitochondrial Pathophysiol Unit, S-22184 Lund, Sweden Univ Copenhagen, Rigshosp, Intens Care Unit 4131, DK-2100 Copenhagen, Denmark Lund Univ, Skane Univ Hosp, Dept Pediat, S-22185 Lund, Sweden Tokyo Med Univ, Dept Anesthesiol, Tokyo 1608402, Japan Lund Univ, Skane Univ Hosp, Dept Pediat Surg, S-22185 Lund, Sweden Lund Univ, Skane Univ Hosp, Dept Clin Physiol, S-22185 Lund, Sweden Univ Helsinki, Childrens Hosp, Helsinki Univ Hosp, FIN-00029 Helsinki, Finland Lund Univ, Skane Univ Hosp, Dept Clin Neurophysiol, S-22185 Lund, Swedenen
dc.identifier.journalMitochondrionen
dc.rights.accessNational Consortium - Landsaðganguren
html.description.abstractStudying whole cell preparations with intact mitochondria and respiratory complexes has a clear benefit compared to isolated or disrupted mitochondria due to the dynamic interplay between mitochondria and other cellular compartments. Platelet mitochondria have a potential to serve as a source of human viable mitochondria when studying mitochondrial physiology and pathogenic mechanisms, as well as for the diagnostics of mitochondrial diseases. The objective of the present study was to perform a detailed evaluation of platelet mitochondrial respiration using high-resolution respirometry. Further, we aimed to explore the limits of sample size and the impact of storage as well as to establish a wide range of reference data from different pediatric and adult cohorts. Our results indicate that platelet mitochondria are well suited for ex-vivo analysis with the need for minute sample amounts and excellent reproducibility and stability.


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