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dc.contributor.authorJonsdottir-Buch, Sandra Mjoll
dc.contributor.authorLieder, Ramona
dc.contributor.authorSigurjonsson, Olafur Eysteinn
dc.date.accessioned2014-05-27T09:44:42Z
dc.date.available2014-05-27T09:44:42Z
dc.date.issued2013-07
dc.date.submitted2014-05-27
dc.identifier.citationPLoS ONE 2013, 8(7):e68984en
dc.identifier.issn1932-6203
dc.identifier.pmid23874839
dc.identifier.doi10.1371/journal.pone.0068984
dc.identifier.urihttp://hdl.handle.net/2336/317454
dc.descriptionTo access publisher's full text version of this article, please click on the hyperlink in Additional Links field or click on the hyperlink at the top of the page marked Files. This article is open access.en
dc.description.abstractMesenchymal stem cells are promising candidates in regenerative cell therapy. Conventional culture methods involve the use of animal substances, specifically fetal bovine serum as growth supplement. Since the use of animal-derived products is undesirable for human applications, platelet lysates produced from human platelets are an attractive alternative. This is especially true if platelet lysates from already approved transfusion units at blood banks can be utilized. The purpose of this study was to produce human platelet lysates from expired, blood bank-approved platelet concentrates and evaluate their use as growth supplement in the culture of mesenchymal stem cells.
dc.description.abstractIn this study, bone marrow-derived mesenchymal stem cells were cultured with one of three culture supplements; fetal bovine serum, lysates from freshly prepared human platelet concentrates, or lysates from expired human platelet concentrates. The effects of these platelet-derived culture supplements on basic mesenchymal stem cell characteristics were evaluated. All cultures maintained the typical mesenchymal stem cell surface marker expression, trilineage differentiation potential, and the ability to suppress in vitro immune responses. However, mesenchymal stem cells supplemented with platelet lysates proliferated faster than traditionally cultured cells and increased the expression of the osteogenic marker gene RUNX-2; yet no difference between the use of fresh and expired platelet concentrates was observed.
dc.description.abstractOur findings suggest that human platelet lysates produced from expired platelet concentrates can be used as an alternative to fetal bovine serum for mesenchymal stem cell culture to the same extent as lysates from fresh platelets.
dc.description.sponsorshipLandspitali University Hospital research funden
dc.language.isoenen
dc.publisherPublic Library Scienceen
dc.relation.urlhttp://dx.doi.org/10.1371/journal.pone.0068984en
dc.relation.urlhttp://www.plosone.org/article/fetchObject.action?uri=info%3Adoi%2F10.1371%2Fjournal.pone.0068984&representation=PDFen
dc.relation.urlhttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC3708923/pdf/pone.0068984.pdfen
dc.rightsopenAccessen
dc.subject.meshAlkaline Phosphataseen
dc.subject.meshBiological Markersen
dc.subject.meshBlood Plateletsen
dc.subject.meshCell Culture Techniquesen
dc.subject.meshCell Differentiationen
dc.subject.meshCell Extractsen
dc.subject.meshCulture Mediaen
dc.subject.meshHumansen
dc.subject.meshMesenchymal Stromal Cellsen
dc.subject.meshOsteogenesisen
dc.titlePlatelet lysates produced from expired platelet concentrates support growth and osteogenic differentiation of mesenchymal stem cells.en
dc.typeArticleen
dc.contributor.departmentLandspitali Univ Hosp, Blood Bank, REModel Lab, Reykjavik, Iceland Univ Iceland, Dept Biomed Sci, Fac Med, Reykjavik, Iceland Univ Reykjavik, Sch Sci & Engn, Reykjavik, Icelanden
dc.identifier.journalPloS oneen
dc.rights.accessOpen Accessen
refterms.dateFOA2018-09-12T13:20:06Z
html.description.abstractMesenchymal stem cells are promising candidates in regenerative cell therapy. Conventional culture methods involve the use of animal substances, specifically fetal bovine serum as growth supplement. Since the use of animal-derived products is undesirable for human applications, platelet lysates produced from human platelets are an attractive alternative. This is especially true if platelet lysates from already approved transfusion units at blood banks can be utilized. The purpose of this study was to produce human platelet lysates from expired, blood bank-approved platelet concentrates and evaluate their use as growth supplement in the culture of mesenchymal stem cells.
html.description.abstractIn this study, bone marrow-derived mesenchymal stem cells were cultured with one of three culture supplements; fetal bovine serum, lysates from freshly prepared human platelet concentrates, or lysates from expired human platelet concentrates. The effects of these platelet-derived culture supplements on basic mesenchymal stem cell characteristics were evaluated. All cultures maintained the typical mesenchymal stem cell surface marker expression, trilineage differentiation potential, and the ability to suppress in vitro immune responses. However, mesenchymal stem cells supplemented with platelet lysates proliferated faster than traditionally cultured cells and increased the expression of the osteogenic marker gene RUNX-2; yet no difference between the use of fresh and expired platelet concentrates was observed.
html.description.abstractOur findings suggest that human platelet lysates produced from expired platelet concentrates can be used as an alternative to fetal bovine serum for mesenchymal stem cell culture to the same extent as lysates from fresh platelets.


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