Pneumococcal pneumonia and bacteremia model in mice for the analysis of protective antibodies
Average rating
Cast your vote
You can rate an item by clicking the amount of stars they wish to award to this item.
When enough users have cast their vote on this item, the average rating will also be shown.
Star rating
Your vote was cast
Thank you for your feedback
Thank you for your feedback
Issue Date
2000-08-01
Metadata
Show full item recordCitation
Microb. Pathog. 2000, 29(2):81-91Abstract
Pneumococci cause infection by colonizing the nasopharynx and invading the mucosal surfaces. Infection models in mice, where the natural route of infection is mimicked, may be useful to study antibody mediated protection against pneumococcal pneumonia and bacteremia. We have established a pneumococcal pneumonia and bacteremia model in mice and investigated the protective capacity of human antibodies. Intranasal challenge with serotypes 1, 3, 6A and 8 caused lung infection and bacteremia which was lethal. Serotype 6B caused low, but detectable, infection and other serotypes tested were not virulent. Passive immunization with a human IgG preparation i.p. protected mice in a dose dependent manner against bacteremia caused by the virulent serotypes (except serotype 3) and partially or completely cleared pneumococci from the lungs of mice infected with serotypes 1, 6A and 8. Adsorption of antibodies with homologous capsular polysaccharides eliminated protection against disease but adsorption with cell wall polysaccharides (CWPS) did not. Furthermore, a good correlation was observed between protection of sera in vivo and opsonic activity in vitro. The results indicate that the model may be useful to analyse the levels, isotypes, specificity and other characteristics of human antibodies which protect against pneumococcal infection and to evaluate the protective potential of pneumococcal vaccine candidates.Description
To access publisher full text version of this article. Please click on the hyperlink in Additional Links fieldAdditional Links
http://www.sciencedirect.com/science/article/B6WN6-45C0RH1-11/2/565707e9e4dc849776f93f54fc6aade6ae974a485f413a2113503eed53cd6c53
10.1006/mpat.2000.0363
Scopus Count
Collections
Related articles
- Serum samples from infants vaccinated with a pneumococcal conjugate vaccine, PncT, protect mice against invasive infection caused by Streptococcus pneumoniae serotypes 6A and 6B.
- Authors: Saeland E, Jakobsen H, Ingolfsdottir G, Sigurdardottir ST, Jonsdottir I
- Issue date: 2001 Jan 15
- Enhancement of Streptococcus pneumoniae serotype 6B infection in mice after passive immunization with human serum.
- Authors: Aaberge IS, Hvalbye B, Løvik M
- Issue date: 1996 Aug
- Correlation of opsonophagocytosis and passive protection assays using human anticapsular antibodies in an infant mouse model of bacteremia for Streptococcus pneumoniae.
- Authors: Johnson SE, Rubin L, Romero-Steiner S, Dykes JK, Pais LB, Rizvi A, Ades E, Carlone GM
- Issue date: 1999 Jul
- Resistance of young mice to pneumococcal infection can be improved by oral vaccination with recombinant Lactococcus lactis.
- Authors: Villena J, Medina M, Racedo S, Alvarez S
- Issue date: 2010 Feb
- Antibodies against pneumococcal C-polysaccharide are not protective.
- Authors: Nielsen SV, Sørensen UB, Henrichsen J
- Issue date: 1993 Apr