Neonatal immune response and serum bactericidal activity induced by a meningococcal conjugate vaccine is enhanced by LT-K63 and CpG2006
Cast your vote
You can rate an item by clicking the amount of stars they wish to award to this item.
When enough users have cast their vote on this item, the average rating will also be shown.
Your vote was cast
Thank you for your feedback
Thank you for your feedback
AuthorsBrynjolfsson, Siggeir F
Bjarnarson, Stefania P
Del Giudice, Giuseppe
MetadataShow full item record
CitationVaccine 2008, 26(35):4557-62
AbstractNeonates have a poorly developed immune system. Therefore it is important to develop vaccination strategies that induce protective immunity and immunological memory against pathogens early in life. The immunogenicity of a meningococcal serogroup C polysaccharide conjugate (MenC-CRM(197)) was assessed in neonatal mice, and effects of LT-K63 and CpG2006 and immunisation routes were compared. Neonatal mice were primed subcutaneously (s.c.) or intranasally (i.n.) with MenC-CRM(197) with or without LT-K63 or CpG2006 and re-immunised 16 and 30 days later by the same route and formulation. Antibody levels were measured and generation of immunological memory assessed by affinity maturation and kinetics of the Ab response. Serum bactericidal activity (SBA) was measured to evaluate protective efficacy. The second and third dose of MenC-CRM(197) mixed with either LT-K63 or CpG2006 induced a rapid increase in MenC-specific IgG antibodies, to levels higher than elicited by MenC-CRM(197) alone (P<0.01) and in unimmunised mice (P<0.001), indicating efficient generation of memory by priming through both s.c. and i.n. routes. SBA was detected after three s.c. immunisations with MenC-CRM(197) s.c. alone. However, only two doses of MenC-CRM(197)+LT-K63 or MenC-CRM(197)+CpG2006 were needed to induce SBA levels>16. LT-K63 and CpG2006 enhanced neonatal antibody responses, affinity maturation, immunological memory to the conjugate MenC-CRM(197) and protective immunity. These results encourage the development of neonatal vaccination strategies to induce protective immunity and immunological memory against meningococcal disease.
DescriptionTo access publisher full text version of this article. Please click on the hyperlink in Additional Links field
- Concomitant administration of Mycobacterium bovis BCG with the meningococcal C conjugate vaccine to neonatal mice enhances antibody response and protective efficacy.
- Authors: Brynjolfsson SF, Bjarnarson SP, Mori E, Del Giudice G, Jonsdottir I
- Issue date: 2011 Nov
- The advantage of mucosal immunization for polysaccharide-specific memory responses in early life.
- Authors: Bjarnarson SP, Jakobsen H, Del Giudice G, Trannoy E, Siegrist CA, Jonsdottir I
- Issue date: 2005 Apr
- Effects of LT-K63 and CpG2006 on phenotype and function of murine neonatal lymphoid cells.
- Authors: Olafsdottir TA, Hannesdottir SG, Giudice GD, Trannoy E, Jonsdottir I
- Issue date: 2007 Oct
- Intranasal immunization of mice with recombinant Streptococcus gordonii expressing NadA of Neisseria meningitidis induces systemic bactericidal antibodies and local IgA.
- Authors: Ciabattini A, Giomarelli B, Parigi R, Chiavolini D, Pettini E, Aricò B, Giuliani MM, Santini L, Medaglini D, Pozzi G
- Issue date: 2008 Aug 5
- Kinetics of antibody responses after primary immunization with meningococcal serogroup C conjugate vaccine or secondary immunization with either conjugate or polysaccharide vaccine in adults.
- Authors: de Voer RM, van der Klis FR, Engels CW, Schepp RM, van de Kassteele J, Sanders EA, Rijkers GT, Berbers GA
- Issue date: 2009 Nov 23