The effect of global SSTR5 gene ablation on the endocrine pancreas and glucose regulation in aging mice
Name:
Publisher version
View Source
Access full-text PDFOpen Access
View Source
Check access options
Check access options
Average rating
Cast your vote
You can rate an item by clicking the amount of stars they wish to award to this item.
When enough users have cast their vote on this item, the average rating will also be shown.
Star rating
Your vote was cast
Thank you for your feedback
Thank you for your feedback
Issue Date
2005-11-01
Metadata
Show full item recordCitation
J. Surg. Res. 2005, 129(1):64-72Abstract
INTRODUCTION: The purpose of this study was to examine the effect of global gene ablation of SSTR5 on the endocrine pancreas, insulin secretion, and glucose tolerance in aging mice, as SSTR5 is a primary regulator of insulin secretion in the mouse pancreas. METHODS: Global SSTR5-/- mice were generated and genotypes were verified using Southern blot and RT-PCR. Glucose tolerance and in vivo insulin secretion in SSTR5-/- and WT mice were examined using intraperitoneal glucose tolerance test (IPGTT;1.2-2.0 mg/kg) at 3 and 12 months of age (n = 8 per group). Basal and glucose-stimulated insulin secretion in vitro was studied using the isolated perfused mouse pancreas model at 3 and 12 months. Pancreata were removed and levels of insulin, glucagon, somatostatin, and SSTR1 were studied using immunohistochemical analysis along with H&E staining of the pancreata. RESULTS: Genotyping verified the absence of SSTR5 in SSTR5-/- mice. IPGTT demonstrated that 3-month-old SSTR5-/- mice were glucose intolerant despite similar insulin secretion both in vivo and in vitro and enlarged islets. At 12 months of age, SSTR5-/- mice had basal hypoglycemia and improved glucose intolerance associated with hyperinsulinemia in vivo and in vitro and enlarged islets. SSTR5-/- mice had increased insulin clearance at 3 and 12 months of age. SSTR1 expression was significantly increased in islets at 3 months of age, but was nearly absent in islets at 12 months of age, as was somatostatin staining in SSTR5-/- mice. CONCLUSIONS: These results suggest that both SSTR5 and SSTR1 play a pivotal role in insulin secretion and glucose regulation in mice and that their regulatory effects are age-related.Description
To access publisher full text version of this article. Please click on the hyperlink in Additional Links fieldAdditional Links
http://www.sciencedirect.com/science/article/B6WM6-4GNCJ5F-2/2/5912ba39daca1f23b5d9d3a010ca4242ae974a485f413a2113503eed53cd6c53
10.1016/j.jss.2005.05.024
Scopus Count
Collections
Related articles
- Double-gene ablation of SSTR1 and SSTR5 results in hyperinsulinemia and improved glucose tolerance in mice.
- Authors: Wang XP, Norman MA, Yang J, Cheung A, Moldovan S, Demayo FJ, Brunicardi FC
- Issue date: 2004 Sep
- SSTR5 ablation in islet results in alterations in glucose homeostasis in mice.
- Authors: Wang XP, Yang J, Norman MA, Magnusson J, DeMayo FJ, Brunicardi FC
- Issue date: 2005 Jun 6
- Sulfonylurea receptor knockout causes glucose intolerance in mice that is not alleviated by concomitant somatostatin subtype receptor 5 knockout.
- Authors: Norman M, Moldovan S, Seghers V, Wang XP, DeMayo FJ, Brunicardi FC
- Issue date: 2002 Jun
- Alterations in glucose homeostasis in SSTR1 gene-ablated mice.
- Authors: Wang XP, Norman M, Yang J, Magnusson J, Kreienkamp HJ, Richter D, DeMayo FJ, Brunicardi FC
- Issue date: 2006 Mar 9
- Characterization of somatostatin receptor subtype-specific regulation of insulin and glucagon secretion: an in vitro study on isolated human pancreatic islets.
- Authors: Singh V, Brendel MD, Zacharias S, Mergler S, Jahr H, Wiedenmann B, Bretzel RG, Plöckinger U, Strowski MZ
- Issue date: 2007 Feb