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dc.contributor.authorPaglia, Giuseppe
dc.contributor.authorSigurjónsson, Ólafur E
dc.contributor.authorRolfsson, Óttar
dc.contributor.authorHansen, Morten Bagge
dc.contributor.authorBrynjólfsson, Sigurður
dc.contributor.authorGudmundsson, Sveinn
dc.contributor.authorPalsson, Bernhard O
dc.date.accessioned2015-08-17T15:03:15Zen
dc.date.available2015-08-17T15:03:15Zen
dc.date.issued2015-02en
dc.date.submitted2015en
dc.identifier.citationTransfusion 2015, 55 (2):301-13en
dc.identifier.issn1537-2995en
dc.identifier.pmid25156572en
dc.identifier.doi10.1111/trf.12834en
dc.identifier.urihttp://hdl.handle.net/2336/574899en
dc.descriptionTo access publisher's full text version of this article click on the hyperlink at the bottom of the pageen
dc.description.abstractPlatelet concentrates (PCs) can be prepared using three methods: platelet (PLT)-rich plasma, apheresis, and buffy coat. The aim of this study was to obtain a comprehensive data set that describes metabolism of buffy coat-derived PLTs during storage and to compare it with a previously published parallel data set obtained for apheresis-derived PLTs.
dc.description.abstractDuring storage we measured more than 150 variables in 8 PLT units, prepared by the buffy coat method. Samples were collected at seven different time points resulting in a data set containing more than 8000 measurements. This data set was obtained by combining a series of standard quality control assays to monitor the quality of stored PLTs and a deep coverage metabolomics study using liquid chromatography coupled with mass spectrometry.
dc.description.abstractStored PLTs showed a distinct metabolic transition occurring 4 days after their collection. The transition was evident in PLT produced by both production methods. Apheresis-derived PLTs showed a clearer phenotype of PLT activation during early days of storage. The activated phenotype of apheresis PLTs was accompanied by a higher metabolic activity, especially related to glycolysis and the tricarboxylic acid cycle. Moreover, the extent of the activation differed between bags resulting in interbag variability in the storage lesion of apheresis-prepared PLTs. This may be related to donor-related polymorphism.
dc.description.abstractThis study demonstrated two discrete metabolic phenotypes in stored PLTs prepared with both apheresis and buffy coat methods. PLT activation occurs during the first metabolic phenotype and might lead to a low reproducibility of the apheresis PCs.
dc.description.sponsorshipinfo:eu-repo/grantAgreement/EC/FP7/232816en
dc.language.isoenen
dc.publisherWiley-Blackwellen
dc.relationinfo:eu-repo/grantAgreement/EC/FP7/232816en
dc.relation.urlhttp://dx.doi.org/ 10.1111/trf.12834en
dc.relation.urlhttp://onlinelibrary.wiley.com/doi/10.1111/trf.12834/epdfen
dc.rightsArchived with thanks to Transfusionen
dc.subject.meshAdulten
dc.subject.meshBlood Buffy Coaten
dc.subject.meshBlood Plateletsen
dc.subject.meshBlood Preservationen
dc.subject.meshFemaleen
dc.subject.meshHumansen
dc.subject.meshMaleen
dc.subject.meshMetabolomeen
dc.subject.meshMetabolomicsen
dc.subject.meshPlatelet Activationen
dc.subject.meshPlateletpheresisen
dc.subject.meshTime Factorsen
dc.titleMetabolomic analysis of platelets during storage: a comparison between apheresis- and buffy coat-derived platelet concentrates.en
dc.typeArticleen
dc.contributor.department[ 1 ] Univ Iceland, Ctr Syst Biol, Reykjavik, Iceland [ 2 ] Landspitali Univ Hosp, Blood Bank, IS-105 Reykjavik, Iceland [ 3 ] Reykjavik Univ, Sch Sci & Engn, Reykjavik, Iceland [ 4 ] Copenhagen Univ Hosp, Rigshosp, Dept Clin Immunol, Copenhagen, Denmarken
dc.identifier.journalTransfusionen
dc.rights.accessNational Consortium - Landsaðganguren
html.description.abstractPlatelet concentrates (PCs) can be prepared using three methods: platelet (PLT)-rich plasma, apheresis, and buffy coat. The aim of this study was to obtain a comprehensive data set that describes metabolism of buffy coat-derived PLTs during storage and to compare it with a previously published parallel data set obtained for apheresis-derived PLTs.
html.description.abstractDuring storage we measured more than 150 variables in 8 PLT units, prepared by the buffy coat method. Samples were collected at seven different time points resulting in a data set containing more than 8000 measurements. This data set was obtained by combining a series of standard quality control assays to monitor the quality of stored PLTs and a deep coverage metabolomics study using liquid chromatography coupled with mass spectrometry.
html.description.abstractStored PLTs showed a distinct metabolic transition occurring 4 days after their collection. The transition was evident in PLT produced by both production methods. Apheresis-derived PLTs showed a clearer phenotype of PLT activation during early days of storage. The activated phenotype of apheresis PLTs was accompanied by a higher metabolic activity, especially related to glycolysis and the tricarboxylic acid cycle. Moreover, the extent of the activation differed between bags resulting in interbag variability in the storage lesion of apheresis-prepared PLTs. This may be related to donor-related polymorphism.
html.description.abstractThis study demonstrated two discrete metabolic phenotypes in stored PLTs prepared with both apheresis and buffy coat methods. PLT activation occurs during the first metabolic phenotype and might lead to a low reproducibility of the apheresis PCs.


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