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Ion mobility-derived collision cross section as an additional measure for lipid fingerprinting and identification.

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Authors
Paglia, Giuseppe
Angel, Peggi
Williams, Jonathan P
Richardson, Keith
Olivos, Hernando J
Thompson, J Will
Menikarachchi, Lochana
Lai, Steven
Walsh, Callee
Moseley, Arthur
Plumb, Robert S
Grant, David F
Palsson, Bernhard O
Langridge, James
Geromanos, Scott
Astarita, Giuseppe
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Issue Date
2015-01-20

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Citation
Anal. Chem. 2015, 87 (2):1137-44
Abstract
Despite recent advances in analytical and computational chemistry, lipid identification remains a significant challenge in lipidomics. Ion-mobility spectrometry provides an accurate measure of the molecules' rotationally averaged collision cross-section (CCS) in the gas phase and is thus related to ionic shape. Here, we investigate the use of CCS as a highly specific molecular descriptor for identifying lipids in biological samples. Using traveling wave ion mobility mass spectrometry (MS), we measured the CCS values of over 200 lipids within multiple chemical classes. CCS values derived from ion mobility were not affected by instrument settings or chromatographic conditions, and they were highly reproducible on instruments located in independent laboratories (interlaboratory RSD < 3% for 98% of molecules). CCS values were used as additional molecular descriptors to identify brain lipids using a variety of traditional lipidomic approaches. The addition of CCS improved the reproducibility of analysis in a liquid chromatography-MS workflow and maximized the separation of isobaric species and the signal-to-noise ratio in direct-MS analyses (e.g., "shotgun" lipidomics and MS imaging). These results indicate that adding CCS to databases and lipidomics workflows increases the specificity and selectivity of analysis, thus improving the confidence in lipid identification compared to traditional analytical approaches. The CCS/accurate-mass database described here is made publicly available.
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To access publisher's full text version of this article, please click on the hyperlink in Additional Links field or click on the hyperlink at the top of the page marked Files. This article is open access.
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http://dx.doi.org/10.1021/ac503715v
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4302848/
http://pubs.acs.org/doi/pdf/10.1021/ac503715v
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openAccess
ae974a485f413a2113503eed53cd6c53
10.1021/ac503715v
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