Kinetic analysis of gluconate phosphorylation by human gluconokinase using isothermal titration calorimetry.
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2015-11-30
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FEBS Lett. 2015, 589 (23):3548-55Abstract
Gluconate is a commonly encountered nutrient, which is degraded by the enzyme gluconokinase to generate 6-phosphogluconate. Here we used isothermal titration calorimetry to study the properties of this reaction. ΔH, KM and kcat are reported along with substrate binding data. We propose that the reaction follows a ternary complex mechanism, with ATP binding first. The reaction is inhibited by gluconate, as it binds to an Enzyme-ADP complex forming a dead-end complex. The study exemplifies that ITC can be used to determine mechanisms of enzyme catalyzed reactions, for which it is currently not commonly applied.Description
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http://dx.doi.org/10.1016/j.febslet.2015.10.024Rights
restrictedAccessae974a485f413a2113503eed53cd6c53
10.1016/j.febslet.2015.10.024
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